Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples
We report the design of a sensitive, electrochemical aptasensor for detection of ochratoxin A (OTA) with an extraordinary tunable dynamic sensing range. This electrochemical aptasensor is constructed based on the target induced aptamer-folding detection mechanism and the recognition between OTA and...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2017-06-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016301306 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557594579828736 |
|---|---|
| author | Lin Cheng Hao Qu Jun Teng Li Yao Feng Xue Wei Chen |
| author_facet | Lin Cheng Hao Qu Jun Teng Li Yao Feng Xue Wei Chen |
| author_sort | Lin Cheng |
| collection | DOAJ |
| description | We report the design of a sensitive, electrochemical aptasensor for detection of ochratoxin A (OTA) with an extraordinary tunable dynamic sensing range. This electrochemical aptasensor is constructed based on the target induced aptamer-folding detection mechanism and the recognition between OTA and its aptamers results in the conformational change of the aptamer probe and thus signal changes for measurement. The dynamic sensing range of the electrochemical aptasensor is successfully tuned by introduction of free assistant aptamer probes in the sensing system. Our electrochemical aptasensor shows an extraordinary dynamic sensing range of 11-order magnitude of OTA concentration from 10−8 to 102 ng/g. Of great significance, the signal response in all OTA concentration ranges is at the same current scale, demonstrating that our sensing protocol in this research could be applied for accurate detections of OTA in a broad range without using any complicated treatment of signal amplification. Finally, OTA spiked red wine and maize samples in different dynamic sensing ranges are determined with the electrochemical aptasensor under optimized sensing conditions. This tuning strategy of dynamic sensing range may offer a promising platform for electrochemical aptasensor optimizations in practical applications. |
| format | Article |
| id | doaj-art-b05d1fab081a444bac9870a57e7c769f |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b05d1fab081a444bac9870a57e7c769f2025-02-03T04:08:01ZengTsinghua University PressFood Science and Human Wellness2213-45302017-06-0162707610.1016/j.fshw.2017.04.002Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samplesLin Cheng0Hao Qu1Jun Teng2Li Yao3Feng Xue4Wei Chen5School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, ChinaSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, ChinaSchool of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, ChinaWe report the design of a sensitive, electrochemical aptasensor for detection of ochratoxin A (OTA) with an extraordinary tunable dynamic sensing range. This electrochemical aptasensor is constructed based on the target induced aptamer-folding detection mechanism and the recognition between OTA and its aptamers results in the conformational change of the aptamer probe and thus signal changes for measurement. The dynamic sensing range of the electrochemical aptasensor is successfully tuned by introduction of free assistant aptamer probes in the sensing system. Our electrochemical aptasensor shows an extraordinary dynamic sensing range of 11-order magnitude of OTA concentration from 10−8 to 102 ng/g. Of great significance, the signal response in all OTA concentration ranges is at the same current scale, demonstrating that our sensing protocol in this research could be applied for accurate detections of OTA in a broad range without using any complicated treatment of signal amplification. Finally, OTA spiked red wine and maize samples in different dynamic sensing ranges are determined with the electrochemical aptasensor under optimized sensing conditions. This tuning strategy of dynamic sensing range may offer a promising platform for electrochemical aptasensor optimizations in practical applications.http://www.sciencedirect.com/science/article/pii/S2213453016301306Electrochemical aptasensorTunable dynamic detection rangeOTA detectionExtraordinary dynamic range |
| spellingShingle | Lin Cheng Hao Qu Jun Teng Li Yao Feng Xue Wei Chen Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples Food Science and Human Wellness Electrochemical aptasensor Tunable dynamic detection range OTA detection Extraordinary dynamic range |
| title | Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples |
| title_full | Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples |
| title_fullStr | Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples |
| title_full_unstemmed | Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples |
| title_short | Extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin A in food samples |
| title_sort | extraordinary tunable dynamic range of electrochemical aptasensor for accurate detection of ochratoxin a in food samples |
| topic | Electrochemical aptasensor Tunable dynamic detection range OTA detection Extraordinary dynamic range |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016301306 |
| work_keys_str_mv | AT lincheng extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples AT haoqu extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples AT junteng extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples AT liyao extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples AT fengxue extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples AT weichen extraordinarytunabledynamicrangeofelectrochemicalaptasensorforaccuratedetectionofochratoxinainfoodsamples |