IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania
The aim of this study was to identify the replicon types of plasmids, conjugation efficiencies, and the complement of antibiotic resistance genes for a panel of multidrug resistant E. coli isolates from surface waters in northern Tanzania. Standard membrane filtration was used to isolate and uidA PC...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/3103672 |
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| author | Beatus Lyimo Joram Buza Murugan Subbiah Sylivester Temba Honest Kipasika Woutrina Smith Douglas R. Call |
| author_facet | Beatus Lyimo Joram Buza Murugan Subbiah Sylivester Temba Honest Kipasika Woutrina Smith Douglas R. Call |
| author_sort | Beatus Lyimo |
| collection | DOAJ |
| description | The aim of this study was to identify the replicon types of plasmids, conjugation efficiencies, and the complement of antibiotic resistance genes for a panel of multidrug resistant E. coli isolates from surface waters in northern Tanzania. Standard membrane filtration was used to isolate and uidA PCR was used to confirm the identity of strains as E. coli. Antibiotic susceptibility was determined by breakpoint assay and plasmid conjugation was determined by filter-mating experiments. PCR and sequencing were used to identify resistance genes and PCR-based replicon typing was used to determine plasmid types. Filter mating experiments indicated conjugation efficiencies ranged from 10−1 to 10−7. Over 80% of the donor cells successfully passed their resistance traits and eleven different replicon types were detected (IncI1, FIC, P, FIIA, A/C, FIB, FIA, H12, K/B B/O, and N). IncF plasmids were most commonly detected (49% of isolates), followed by types IncI1 and IncA/C. Detection of these public health-relevant conjugative plasmids and antibiotic resistant traits in Tanzanian water suggests the possible pollution of these water sources from human, livestock, and wild animal wastes and also shows the potential of these water sources in the maintenance and transmission of these resistance traits between environments, animals, and people. |
| format | Article |
| id | doaj-art-affdcd5f6a71492e90766a9bbd323e4c |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-affdcd5f6a71492e90766a9bbd323e4c2025-02-03T01:25:45ZengWileyInternational Journal of Microbiology1687-918X1687-91982016-01-01201610.1155/2016/31036723103672IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern TanzaniaBeatus Lyimo0Joram Buza1Murugan Subbiah2Sylivester Temba3Honest Kipasika4Woutrina Smith5Douglas R. Call6Nelson Mandela African Institution of Science and Technology, 447 Arusha, TanzaniaNelson Mandela African Institution of Science and Technology, 447 Arusha, TanzaniaPaul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USANelson Mandela African Institution of Science and Technology, 447 Arusha, TanzaniaNelson Mandela African Institution of Science and Technology, 447 Arusha, TanzaniaOne Health Institute, School of Veterinary Medicine, University of California, Davis, CA 95616, USANelson Mandela African Institution of Science and Technology, 447 Arusha, TanzaniaThe aim of this study was to identify the replicon types of plasmids, conjugation efficiencies, and the complement of antibiotic resistance genes for a panel of multidrug resistant E. coli isolates from surface waters in northern Tanzania. Standard membrane filtration was used to isolate and uidA PCR was used to confirm the identity of strains as E. coli. Antibiotic susceptibility was determined by breakpoint assay and plasmid conjugation was determined by filter-mating experiments. PCR and sequencing were used to identify resistance genes and PCR-based replicon typing was used to determine plasmid types. Filter mating experiments indicated conjugation efficiencies ranged from 10−1 to 10−7. Over 80% of the donor cells successfully passed their resistance traits and eleven different replicon types were detected (IncI1, FIC, P, FIIA, A/C, FIB, FIA, H12, K/B B/O, and N). IncF plasmids were most commonly detected (49% of isolates), followed by types IncI1 and IncA/C. Detection of these public health-relevant conjugative plasmids and antibiotic resistant traits in Tanzanian water suggests the possible pollution of these water sources from human, livestock, and wild animal wastes and also shows the potential of these water sources in the maintenance and transmission of these resistance traits between environments, animals, and people.http://dx.doi.org/10.1155/2016/3103672 |
| spellingShingle | Beatus Lyimo Joram Buza Murugan Subbiah Sylivester Temba Honest Kipasika Woutrina Smith Douglas R. Call IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania International Journal of Microbiology |
| title | IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania |
| title_full | IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania |
| title_fullStr | IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania |
| title_full_unstemmed | IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania |
| title_short | IncF Plasmids Are Commonly Carried by Antibiotic Resistant Escherichia coli Isolated from Drinking Water Sources in Northern Tanzania |
| title_sort | incf plasmids are commonly carried by antibiotic resistant escherichia coli isolated from drinking water sources in northern tanzania |
| url | http://dx.doi.org/10.1155/2016/3103672 |
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