Differential Proliferative Characteristics of Alveolar Fibroblasts in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2
Fibroblasts (Fb) from patients with sarcoidosis (SA) and hypersensitivity pneumonitis (HP) exhibited a lower proliferative capacity compared with Fb obtained from control (CO) and diffuse interstitial fibrosis patients (DIF). Proliferation of Fb from SA or lip patients was suppressed by autologous L...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000633 |
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| author | Elizabeth Fireman Shlomo Ben Efraim Joel Greif Hava Peretz Shmuel Kivity Marcel Topilsky Yosef Rodrig A. Yellin Ron N. Apte |
| author_facet | Elizabeth Fireman Shlomo Ben Efraim Joel Greif Hava Peretz Shmuel Kivity Marcel Topilsky Yosef Rodrig A. Yellin Ron N. Apte |
| author_sort | Elizabeth Fireman |
| collection | DOAJ |
| description | Fibroblasts (Fb) from patients with sarcoidosis (SA) and
hypersensitivity pneumonitis (HP) exhibited a lower proliferative
capacity compared with Fb obtained from control (CO) and diffuse
interstitial fibrosis patients (DIF). Proliferation of Fb from SA or
lip patients was suppressed by autologous LPS-stimulated alveolar
macrophages (AM) supernatants but not by those from CO patients.
Similarly, alveolar macrophages (AM) derived supernatant, obtained
from CO, did not suppress the proliferation of SA and HP Fb. AM from
SA and HP patients secreted higher amounts of IL-1α and β
compared with controls and compared with Fb from SA and HP patients.
Steady levels of IL-1α and βmRNA were expressed in
unstimulated and stimulated cultures. Fb from SA and HP patients
could be stimulated by LPS to secrete significantly higher levels of
PGE2 than those detected in supernatants from LPS
stimulated Fb of DIF patients. Only the proliferation of Fb from SA
and HP patients was sensitive to amounts of IL-1 equivalent to those
detected in the lung of these diseases. As SA and HP are two
diseases where irreversible deterioration occurs in only 20%
of the patients, we hypothesize that mediators in the lung may
modulate Fb proliferation. IL-1 of AM origin and PGE2 of
Fb origin secreted at high levels, may be candidates for this
suppression because it was abrogated by anti IL-1β and indomethacin. |
| format | Article |
| id | doaj-art-afc2392aee2543a88115ca0e7a27137d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-afc2392aee2543a88115ca0e7a27137d2025-02-03T01:26:45ZengWileyMediators of Inflammation0962-93511466-18611994-01-013644545210.1155/S0962935194000633Differential Proliferative Characteristics of Alveolar Fibroblasts in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2Elizabeth Fireman0Shlomo Ben Efraim1Joel Greif2Hava Peretz3Shmuel Kivity4Marcel Topilsky5Yosef Rodrig6A. Yellin7Ron N. Apte8Departments of Pulmonary and Allergic Diseases, Ichilov Hospital, 6 Weizmann Street, Tel-Aviv 64239, IsraelDepartment of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, IsraelDepartments of Pulmonary and Allergic Diseases, Ichilov Hospital, 6 Weizmann Street, Tel-Aviv 64239, IsraelClinical Chemistry, Ichilov Hospital, Tel-Aviv Sourasky Medical Center, IsraelDepartments of Pulmonary and Allergic Diseases, Ichilov Hospital, 6 Weizmann Street, Tel-Aviv 64239, IsraelInternal Medicine “H”, Ichilov Hospital, Tel-Aviv Sourasky Medical Center, IsraelInternal Medicine “H”, Ichilov Hospital, Tel-Aviv Sourasky Medical Center, IsraelDepartment of Thoracic Surgery, Shiba Medical Centre, IsraelDepartment of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, IsraelFibroblasts (Fb) from patients with sarcoidosis (SA) and hypersensitivity pneumonitis (HP) exhibited a lower proliferative capacity compared with Fb obtained from control (CO) and diffuse interstitial fibrosis patients (DIF). Proliferation of Fb from SA or lip patients was suppressed by autologous LPS-stimulated alveolar macrophages (AM) supernatants but not by those from CO patients. Similarly, alveolar macrophages (AM) derived supernatant, obtained from CO, did not suppress the proliferation of SA and HP Fb. AM from SA and HP patients secreted higher amounts of IL-1α and β compared with controls and compared with Fb from SA and HP patients. Steady levels of IL-1α and βmRNA were expressed in unstimulated and stimulated cultures. Fb from SA and HP patients could be stimulated by LPS to secrete significantly higher levels of PGE2 than those detected in supernatants from LPS stimulated Fb of DIF patients. Only the proliferation of Fb from SA and HP patients was sensitive to amounts of IL-1 equivalent to those detected in the lung of these diseases. As SA and HP are two diseases where irreversible deterioration occurs in only 20% of the patients, we hypothesize that mediators in the lung may modulate Fb proliferation. IL-1 of AM origin and PGE2 of Fb origin secreted at high levels, may be candidates for this suppression because it was abrogated by anti IL-1β and indomethacin.http://dx.doi.org/10.1155/S0962935194000633 |
| spellingShingle | Elizabeth Fireman Shlomo Ben Efraim Joel Greif Hava Peretz Shmuel Kivity Marcel Topilsky Yosef Rodrig A. Yellin Ron N. Apte Differential Proliferative Characteristics of Alveolar Fibroblasts in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 Mediators of Inflammation |
| title | Differential Proliferative Characteristics of Alveolar Fibroblasts
in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 |
| title_full | Differential Proliferative Characteristics of Alveolar Fibroblasts
in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 |
| title_fullStr | Differential Proliferative Characteristics of Alveolar Fibroblasts
in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 |
| title_full_unstemmed | Differential Proliferative Characteristics of Alveolar Fibroblasts
in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 |
| title_short | Differential Proliferative Characteristics of Alveolar Fibroblasts
in Interstitial Lung Diseases: Regulative Role of IL-1 and PGE2 |
| title_sort | differential proliferative characteristics of alveolar fibroblasts in interstitial lung diseases regulative role of il 1 and pge2 |
| url | http://dx.doi.org/10.1155/S0962935194000633 |
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