Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology
Abstract Background Adult tendon tissue has limited and slow regenerative capacity. Sambucus nigra plant possesses antioxidant and anti‐inflammatory attributes. Objectives This study aimed to evaluate the effect of hydroalcoholic extract of this plant's fruit on superficial digital flexor tendo...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1581 |
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| author | Niusha Asgari Elham Moghtadaei‐Khorasgani Mehrdad Yadegari Omid Taheri‐Boroujeni |
| author_facet | Niusha Asgari Elham Moghtadaei‐Khorasgani Mehrdad Yadegari Omid Taheri‐Boroujeni |
| author_sort | Niusha Asgari |
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| description | Abstract Background Adult tendon tissue has limited and slow regenerative capacity. Sambucus nigra plant possesses antioxidant and anti‐inflammatory attributes. Objectives This study aimed to evaluate the effect of hydroalcoholic extract of this plant's fruit on superficial digital flexor tendon repair in rabbits (SDFT). Methods Twenty‐five male New Zealand white rabbits weighing 1.5–2 kg were selected, quarantined and randomly divided into four groups of six. By performing a partial left posterior limb tenotomy, differentiating the SDFT and creating multiple scrapes were performed. During 3 consecutive days post‐surgery, the positive control group was injected with 0.5 mg/kg dexamethasone, whereas the treatment groups received extract doses of 200 and 400 mg/kg, respectively. The negative control group did not receive any medication. Evaluation of sonographic and histopathological parameters was conducted on days 0, 7 and 28 post‐surgeries. Findings were analysed and compared using SPSS22. Results Both treatment groups showed significant differences in echogenicity, and collagen fibre alignment compared to the control and positive control groups, in sonographic evaluation. Histopathological examination revealed fewer inflammatory cells and increased collagen fibre formation in the treatment groups compared to the other two groups. No significant difference in angiogenesis was observed among the groups on days 7 and 28 (p value <0.05). Conclusions The results indicate that the S. nigra fruit extract, by stimulating collagen synthesis and reducing inflammation, effectively accelerates the healing process of injured tendons. |
| format | Article |
| id | doaj-art-afa96d66c67e42dfb067cd4b21a542e2 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-afa96d66c67e42dfb067cd4b21a542e22025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.1581Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathologyNiusha Asgari0Elham Moghtadaei‐Khorasgani1Mehrdad Yadegari2Omid Taheri‐Boroujeni3Graduated of Veterinary Medicine, Shahrekord Branch Islamic Azad University Shahrekord IranDepartment of Pathobiology, Shahrekord Branch Islamic Azad University Shahrekord IranDepartment of Veterinary Clinical Sciences, Shahrekord Branch Islamic Azad University Shahrekord IranFaculty of Veterinary Medicine, Young Researchers and Elite Club, Shahrekord Branch Islamic Azad University Shahrekord IranAbstract Background Adult tendon tissue has limited and slow regenerative capacity. Sambucus nigra plant possesses antioxidant and anti‐inflammatory attributes. Objectives This study aimed to evaluate the effect of hydroalcoholic extract of this plant's fruit on superficial digital flexor tendon repair in rabbits (SDFT). Methods Twenty‐five male New Zealand white rabbits weighing 1.5–2 kg were selected, quarantined and randomly divided into four groups of six. By performing a partial left posterior limb tenotomy, differentiating the SDFT and creating multiple scrapes were performed. During 3 consecutive days post‐surgery, the positive control group was injected with 0.5 mg/kg dexamethasone, whereas the treatment groups received extract doses of 200 and 400 mg/kg, respectively. The negative control group did not receive any medication. Evaluation of sonographic and histopathological parameters was conducted on days 0, 7 and 28 post‐surgeries. Findings were analysed and compared using SPSS22. Results Both treatment groups showed significant differences in echogenicity, and collagen fibre alignment compared to the control and positive control groups, in sonographic evaluation. Histopathological examination revealed fewer inflammatory cells and increased collagen fibre formation in the treatment groups compared to the other two groups. No significant difference in angiogenesis was observed among the groups on days 7 and 28 (p value <0.05). Conclusions The results indicate that the S. nigra fruit extract, by stimulating collagen synthesis and reducing inflammation, effectively accelerates the healing process of injured tendons.https://doi.org/10.1002/vms3.1581collagendexamethasoneinflammationSambucus nigratendon |
| spellingShingle | Niusha Asgari Elham Moghtadaei‐Khorasgani Mehrdad Yadegari Omid Taheri‐Boroujeni Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology Veterinary Medicine and Science collagen dexamethasone inflammation Sambucus nigra tendon |
| title | Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| title_full | Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| title_fullStr | Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| title_full_unstemmed | Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| title_short | Effect of hydroalcoholic extract of Sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| title_sort | effect of hydroalcoholic extract of sambucus nigra on superficial digital flexor tendon repair in rabbit by ultrasonography and histopathology |
| topic | collagen dexamethasone inflammation Sambucus nigra tendon |
| url | https://doi.org/10.1002/vms3.1581 |
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