The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats
Background and Aim: Diabetes is a main metabolic disorder in middle-aged and older people. Oxidative stress can interfere with insulin signaling and are directly relevant to the incidence of diabetes. The AKT signaling pathways plays a critical role in regulating glucose homeostasis and proliferatio...
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Kurdistan University of Medical Sciences
2024-11-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-8288-en.pdf |
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| author | Maryam Arabloei Sani Zahra Hajebrahimi Parichehreh Yaghmaei Nasim Hayati Roodbari |
| author_facet | Maryam Arabloei Sani Zahra Hajebrahimi Parichehreh Yaghmaei Nasim Hayati Roodbari |
| author_sort | Maryam Arabloei Sani |
| collection | DOAJ |
| description | Background and Aim: Diabetes is a main metabolic disorder in middle-aged and older people. Oxidative stress can interfere with insulin signaling and are directly relevant to the incidence of diabetes. The AKT signaling pathways plays a critical role in regulating glucose homeostasis and proliferation of β cells. P-cymene is an aromatic monotropene with antioxidant and anti-inflammatory properties. In the present study, the therapeutic effects of p-cymene on blood glucose and insulin levels, the antioxidant power of serum (FRAP assay) and the expression of AKT mRNAs in streptozotocin-induced diabetic rats were investigated.
Materials and Methods: Diabetes was induced using the injection of 55 mg/kg streptozotocin in male Wistar rats. Histological, biochemical, and real-time PCR analyses were done to study the effects of metformin (55 mg/kg) and p-cymene (25, 50, and 100 mg/kg) on levels of glucose, insulin, oxidative stress status, and the expression of AKT mRNA.
Results: Streptozotocin increased serum levels of glucose and decreased serum levels of insulin, the antioxidant power of serum, pancreas levels of AKT mRNA, and β island size. Administration of metformin or p-cymene improved the levels of glucose, insulin, AKT mRNA, β island size, and antioxidant power in an independent manner of dose.
Conclusion: These results suggest that p-cymene has hypoglycemic, hyperinsulinemia, and antioxidant properties. It can regulate Akt signaling pathway. Therefore, it may be suggested for the diabetes therapy alone or in combination with metformin. |
| format | Article |
| id | doaj-art-af96630dd81c4698a5285ec6d5b38cd0 |
| institution | Kabale University |
| issn | 1560-652X 2345-4040 |
| language | fas |
| publishDate | 2024-11-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj-art-af96630dd81c4698a5285ec6d5b38cd02025-02-01T09:41:40ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402024-11-012952536The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar RatsMaryam Arabloei Sani0Zahra Hajebrahimi1Parichehreh Yaghmaei2Nasim Hayati Roodbari3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Background and Aim: Diabetes is a main metabolic disorder in middle-aged and older people. Oxidative stress can interfere with insulin signaling and are directly relevant to the incidence of diabetes. The AKT signaling pathways plays a critical role in regulating glucose homeostasis and proliferation of β cells. P-cymene is an aromatic monotropene with antioxidant and anti-inflammatory properties. In the present study, the therapeutic effects of p-cymene on blood glucose and insulin levels, the antioxidant power of serum (FRAP assay) and the expression of AKT mRNAs in streptozotocin-induced diabetic rats were investigated. Materials and Methods: Diabetes was induced using the injection of 55 mg/kg streptozotocin in male Wistar rats. Histological, biochemical, and real-time PCR analyses were done to study the effects of metformin (55 mg/kg) and p-cymene (25, 50, and 100 mg/kg) on levels of glucose, insulin, oxidative stress status, and the expression of AKT mRNA. Results: Streptozotocin increased serum levels of glucose and decreased serum levels of insulin, the antioxidant power of serum, pancreas levels of AKT mRNA, and β island size. Administration of metformin or p-cymene improved the levels of glucose, insulin, AKT mRNA, β island size, and antioxidant power in an independent manner of dose. Conclusion: These results suggest that p-cymene has hypoglycemic, hyperinsulinemia, and antioxidant properties. It can regulate Akt signaling pathway. Therefore, it may be suggested for the diabetes therapy alone or in combination with metformin.http://sjku.muk.ac.ir/article-1-8288-en.pdfdiabetes mellitusp-cymeneoxidative stressakt |
| spellingShingle | Maryam Arabloei Sani Zahra Hajebrahimi Parichehreh Yaghmaei Nasim Hayati Roodbari The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats مجله علمی دانشگاه علوم پزشکی کردستان diabetes mellitus p-cymene oxidative stress akt |
| title | The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats |
| title_full | The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats |
| title_fullStr | The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats |
| title_full_unstemmed | The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats |
| title_short | The Effect of P-Cymene on Glucose, Insulin, Antioxidant Power, and the Level of AKT Gene mRNA in Streptozotocin-Induced Diabetes Model in Male Wistar Rats |
| title_sort | effect of p cymene on glucose insulin antioxidant power and the level of akt gene mrna in streptozotocin induced diabetes model in male wistar rats |
| topic | diabetes mellitus p-cymene oxidative stress akt |
| url | http://sjku.muk.ac.ir/article-1-8288-en.pdf |
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