Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain

Introduction: Twenty-five to 29% of the global population experiences pain that motivates seeking emergency services. Objective: To evaluate the effect of schizophyllan glucan polysaccharide (SPG), an isolated β-(1→3),(1→6) glucan polysaccharide of Schizophyllum commune, in acute pain and neuromusc...

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Main Authors: Katherine Plautz, Gabriela Borgmann, Alessandra Betina Gastaldi, Gustavo Schuetzler Gomes Fernandes, Eduardo Manoel Pereira, Samira Dal-Toé de Prá, Nicole Dalonso, Débora Delwing-Dal Magro, Daniela Delwing-de Lima
Format: Article
Language:English
Published: Faculdade de Medicina do ABC 2025-04-01
Series:ABCS Health Sciences
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Online Access:https://www.portalnepas.org.br/abcshs/article/view/2718
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author Katherine Plautz
Gabriela Borgmann
Alessandra Betina Gastaldi
Gustavo Schuetzler Gomes Fernandes
Eduardo Manoel Pereira
Samira Dal-Toé de Prá
Nicole Dalonso
Débora Delwing-Dal Magro
Daniela Delwing-de Lima
author_facet Katherine Plautz
Gabriela Borgmann
Alessandra Betina Gastaldi
Gustavo Schuetzler Gomes Fernandes
Eduardo Manoel Pereira
Samira Dal-Toé de Prá
Nicole Dalonso
Débora Delwing-Dal Magro
Daniela Delwing-de Lima
author_sort Katherine Plautz
collection DOAJ
description Introduction: Twenty-five to 29% of the global population experiences pain that motivates seeking emergency services. Objective: To evaluate the effect of schizophyllan glucan polysaccharide (SPG), an isolated β-(1→3),(1→6) glucan polysaccharide of Schizophyllum commune, in acute pain and neuromuscular performance on pre-clinical models. Methods: Male adult Swiss mice (20-30g, 60 days) were acclimated for a week in groups of 7 per cage before the experiment. SPG was administered, intraperitoneally, at doses of 0.1, 1.0, 3.0, 5.0, 10.0, 30.0, and 100.0 mg/Kg for the writhing test, and 1.0, 10.0, and 30.0 mg/Kg for the formalin and Rotarod tests, respectively. Statistical analysis was performed using one-way ANOVA, followed by the Duncan post hoc test, respectively, as appropriate (p<0.05). Results: Regarding the abdominal writhing test, SPG doses of 1.0, 5.0, 10.0, 30.0, and 100.0 mg/Kg promoted a significant reduction in writing of, respectively, 90.6%, 86.6%, 83.0%, 86.6%, and 76.2%. In the formalin test, the dose of SPG 30 mg/Kg reduced phase II nociception time by 78.0%. Relevant sedation was observed only to SPG 100 mg/Kg in the Rotarod test. Conclusion: SPG showed significant analgesic effects on acute inflammatory pain without causing concomitant central nervous system depression.
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spelling doaj-art-af8d02b0b0dc4f1b902baf801f0ad1f62025-08-20T03:14:23ZengFaculdade de Medicina do ABCABCS Health Sciences2318-49652357-81142025-04-0110.7322/abcshs.2024014.2718Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain Katherine Plautz0Gabriela Borgmann1Alessandra Betina Gastaldi2Gustavo Schuetzler Gomes Fernandes3Eduardo Manoel Pereira4Samira Dal-Toé de Prá5Nicole Dalonso6Débora Delwing-Dal Magro7Daniela Delwing-de Lima8Programa de Pós-Graduação em Saúde e Meio Ambiente, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), BrazilPrograma de Pós-Graduação em Saúde e Meio Ambiente, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), BrazilPrograma de Pós-Graduação em Saúde e Meio Ambiente, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), BrazilDepartamento de Medicina, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), BrazilDepartamento de Farmácia, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), BrazilSecretaria de Saúde, Sociedade Educacional de Joinville (UNISOCIESC) – Joinville (SC), BrazilDepartamento de Ciências Naturais, Universidade de Blumenau (FURB) – Blumenau (SC), BrazilDepartamento de Ciências Naturais, Universidade de Blumenau (FURB) – Blumenau (SC), BrazilPrograma de Pós-Graduação em Saúde e Meio Ambiente, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), Brazil /Departamento de Medicina, Universidade Estadual de Joinville (UNIVILLE) – Joinville (SC), Brazil Introduction: Twenty-five to 29% of the global population experiences pain that motivates seeking emergency services. Objective: To evaluate the effect of schizophyllan glucan polysaccharide (SPG), an isolated β-(1→3),(1→6) glucan polysaccharide of Schizophyllum commune, in acute pain and neuromuscular performance on pre-clinical models. Methods: Male adult Swiss mice (20-30g, 60 days) were acclimated for a week in groups of 7 per cage before the experiment. SPG was administered, intraperitoneally, at doses of 0.1, 1.0, 3.0, 5.0, 10.0, 30.0, and 100.0 mg/Kg for the writhing test, and 1.0, 10.0, and 30.0 mg/Kg for the formalin and Rotarod tests, respectively. Statistical analysis was performed using one-way ANOVA, followed by the Duncan post hoc test, respectively, as appropriate (p<0.05). Results: Regarding the abdominal writhing test, SPG doses of 1.0, 5.0, 10.0, 30.0, and 100.0 mg/Kg promoted a significant reduction in writing of, respectively, 90.6%, 86.6%, 83.0%, 86.6%, and 76.2%. In the formalin test, the dose of SPG 30 mg/Kg reduced phase II nociception time by 78.0%. Relevant sedation was observed only to SPG 100 mg/Kg in the Rotarod test. Conclusion: SPG showed significant analgesic effects on acute inflammatory pain without causing concomitant central nervous system depression. https://www.portalnepas.org.br/abcshs/article/view/2718acute painfungal polysaccharidesSchizophyllummodels, animalanalgesiaanti-inflammatory agents
spellingShingle Katherine Plautz
Gabriela Borgmann
Alessandra Betina Gastaldi
Gustavo Schuetzler Gomes Fernandes
Eduardo Manoel Pereira
Samira Dal-Toé de Prá
Nicole Dalonso
Débora Delwing-Dal Magro
Daniela Delwing-de Lima
Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
ABCS Health Sciences
acute pain
fungal polysaccharides
Schizophyllum
models, animal
analgesia
anti-inflammatory agents
title Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
title_full Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
title_fullStr Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
title_full_unstemmed Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
title_short Schizophyllan Polysaccharide from Schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
title_sort schizophyllan polysaccharide from schizophyllum commune demonstrates antinociceptive effect on preclinical models of acute pain
topic acute pain
fungal polysaccharides
Schizophyllum
models, animal
analgesia
anti-inflammatory agents
url https://www.portalnepas.org.br/abcshs/article/view/2718
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