Identification of Binding Partners of Deafness-Related Protein PDZD7
PDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2018/2062346 |
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| _version_ | 1832548143053406208 |
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| author | Haibo Du Rui Ren Panpan Chen Zhigang Xu Yanfei Wang |
| author_facet | Haibo Du Rui Ren Panpan Chen Zhigang Xu Yanfei Wang |
| author_sort | Haibo Du |
| collection | DOAJ |
| description | PDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1, whirlin, harmonin, SANS, and MYO7A, all belonging to USH proteins. Here, we report the identification of novel PDZD7-binding partners through yeast two-hybrid screening using the first two PDZ domains of PDZD7 as bait. Eleven proteins were identified, most of which have not been reported as PDZD7-binding partners before. Among the identified proteins, ADGRV1, gelsolin, and β-catenin have been shown to play important roles in hearing, whereas the functions of other proteins in the inner ear remain elusive. We confirmed the expression of one candidate PDZD7-binding protein, CADM1, in the mouse inner ear and evaluated the auditory function of Cadm1 knockout mice by performing auditory brainstem response (ABR) measurement. Unexpectedly, Cadm1 knockout mice show normal hearing threshold, which might be explained by the possible compensation by its homologs that are also expressed in the inner ear. Taken together, our work identified several novel PDZD7-binding proteins, which will help us to further understand the role of PDZD7 in hearing transduction. |
| format | Article |
| id | doaj-art-af354263864b446a8856173e877a4165 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-af354263864b446a8856173e877a41652025-02-03T06:42:12ZengWileyNeural Plasticity2090-59041687-54432018-01-01201810.1155/2018/20623462062346Identification of Binding Partners of Deafness-Related Protein PDZD7Haibo Du0Rui Ren1Panpan Chen2Zhigang Xu3Yanfei Wang4Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, ChinaShenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, ChinaShenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, ChinaShenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, ChinaShenzhen Research Institute of Shandong University, Shenzhen, Guangdong 518057, ChinaPDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1, whirlin, harmonin, SANS, and MYO7A, all belonging to USH proteins. Here, we report the identification of novel PDZD7-binding partners through yeast two-hybrid screening using the first two PDZ domains of PDZD7 as bait. Eleven proteins were identified, most of which have not been reported as PDZD7-binding partners before. Among the identified proteins, ADGRV1, gelsolin, and β-catenin have been shown to play important roles in hearing, whereas the functions of other proteins in the inner ear remain elusive. We confirmed the expression of one candidate PDZD7-binding protein, CADM1, in the mouse inner ear and evaluated the auditory function of Cadm1 knockout mice by performing auditory brainstem response (ABR) measurement. Unexpectedly, Cadm1 knockout mice show normal hearing threshold, which might be explained by the possible compensation by its homologs that are also expressed in the inner ear. Taken together, our work identified several novel PDZD7-binding proteins, which will help us to further understand the role of PDZD7 in hearing transduction.http://dx.doi.org/10.1155/2018/2062346 |
| spellingShingle | Haibo Du Rui Ren Panpan Chen Zhigang Xu Yanfei Wang Identification of Binding Partners of Deafness-Related Protein PDZD7 Neural Plasticity |
| title | Identification of Binding Partners of Deafness-Related Protein PDZD7 |
| title_full | Identification of Binding Partners of Deafness-Related Protein PDZD7 |
| title_fullStr | Identification of Binding Partners of Deafness-Related Protein PDZD7 |
| title_full_unstemmed | Identification of Binding Partners of Deafness-Related Protein PDZD7 |
| title_short | Identification of Binding Partners of Deafness-Related Protein PDZD7 |
| title_sort | identification of binding partners of deafness related protein pdzd7 |
| url | http://dx.doi.org/10.1155/2018/2062346 |
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