Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets
This 60-day experiment investigated the influences of growth, fat metabolism and hepatic health of largemouth bass (Micropterus salmoides) fed varying fat diets. 225 fish (34 ± 0.04 g) were divided into three groups and fed diets with fat levels of 10 % (Low-fat diet, LFD), 14 % (Medium-fat diet, MF...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832087569842569216 |
|---|---|
| author | Digen Qin Jiale Chen Junxiong Li Zhiyou Liu Weibin Huang Yuanzhi Yang Jing Zhang Beiping Tan Xiaohui Dong |
| author_facet | Digen Qin Jiale Chen Junxiong Li Zhiyou Liu Weibin Huang Yuanzhi Yang Jing Zhang Beiping Tan Xiaohui Dong |
| author_sort | Digen Qin |
| collection | DOAJ |
| description | This 60-day experiment investigated the influences of growth, fat metabolism and hepatic health of largemouth bass (Micropterus salmoides) fed varying fat diets. 225 fish (34 ± 0.04 g) were divided into three groups and fed diets with fat levels of 10 % (Low-fat diet, LFD), 14 % (Medium-fat diet, MFD) and 18 % (High-fat diet, HFD), respectively. The results revealed that fish fed HFD exhibited significantly better protein efficiency ratio (PER) and lower feed conversion ratio (FCR) than LFD group (P<0.05), but survival rate (SR) and weight gain rate (WGR) were unaffected (P>0.05). Dietary fat levels significantly decreased hepatosomatic index (HSI) of fish (P<0.05), but visceral somatic index (VSI) and condition factor (CF) were unaffected (P>0.05). The contents of fat and ash in whole-body, and hepatic fat contents were increased with dietary fat levels (P<0.05), the contents of moisture and crude protein in whole-body and liver exhibited no substantial variations (P>0.05). Serum biochemical indexes and serum alanine aminotransferase (ALT) activity, liver glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased significantly with rising dietary fat levels (P<0.05), serum aspartate transaminase (AST) and liver superoxide dismutase (SOD) activities had no statistical differences (P>0.05). The expression of srebp-1 and acc in liver were down-regulated, but atgl, aco and bax were up-regulated with rising dietary fat contents (P<0.05). Caspase-3 exhibited a remarkable tendency of initially rising and then declining (P<0.05), the expression of fas, cpt-I, bcl-2 and the ratio of bcl-2/bax were unaffected (P>0.05). The histology of liver indicated the size and quantity of fat droplets increased with increment of dietary fat intake. Conclusion: the dietary fat levels of 14 %-18 % could be conducive to better growth of largemouth bass in this study. |
| format | Article |
| id | doaj-art-aed185ef07d94b07a3753f897cb32793 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-aed185ef07d94b07a3753f897cb327932025-02-06T05:12:03ZengElsevierAquaculture Reports2352-51342025-03-0140102535Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level dietsDigen Qin0Jiale Chen1Junxiong Li2Zhiyou Liu3Weibin Huang4Yuanzhi Yang5Jing Zhang6Beiping Tan7Xiaohui Dong8Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Corresponding author.Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture and Rural Affairs, Zhanjiang 524000, ChinaLaboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture and Rural Affairs, Zhanjiang 524000, China; Corresponding author at: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.This 60-day experiment investigated the influences of growth, fat metabolism and hepatic health of largemouth bass (Micropterus salmoides) fed varying fat diets. 225 fish (34 ± 0.04 g) were divided into three groups and fed diets with fat levels of 10 % (Low-fat diet, LFD), 14 % (Medium-fat diet, MFD) and 18 % (High-fat diet, HFD), respectively. The results revealed that fish fed HFD exhibited significantly better protein efficiency ratio (PER) and lower feed conversion ratio (FCR) than LFD group (P<0.05), but survival rate (SR) and weight gain rate (WGR) were unaffected (P>0.05). Dietary fat levels significantly decreased hepatosomatic index (HSI) of fish (P<0.05), but visceral somatic index (VSI) and condition factor (CF) were unaffected (P>0.05). The contents of fat and ash in whole-body, and hepatic fat contents were increased with dietary fat levels (P<0.05), the contents of moisture and crude protein in whole-body and liver exhibited no substantial variations (P>0.05). Serum biochemical indexes and serum alanine aminotransferase (ALT) activity, liver glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased significantly with rising dietary fat levels (P<0.05), serum aspartate transaminase (AST) and liver superoxide dismutase (SOD) activities had no statistical differences (P>0.05). The expression of srebp-1 and acc in liver were down-regulated, but atgl, aco and bax were up-regulated with rising dietary fat contents (P<0.05). Caspase-3 exhibited a remarkable tendency of initially rising and then declining (P<0.05), the expression of fas, cpt-I, bcl-2 and the ratio of bcl-2/bax were unaffected (P>0.05). The histology of liver indicated the size and quantity of fat droplets increased with increment of dietary fat intake. Conclusion: the dietary fat levels of 14 %-18 % could be conducive to better growth of largemouth bass in this study.http://www.sciencedirect.com/science/article/pii/S2352513424006239Growthfat metabolismhepatic healthfat levelslargemouth bass |
| spellingShingle | Digen Qin Jiale Chen Junxiong Li Zhiyou Liu Weibin Huang Yuanzhi Yang Jing Zhang Beiping Tan Xiaohui Dong Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets Aquaculture Reports Growth fat metabolism hepatic health fat levels largemouth bass |
| title | Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets |
| title_full | Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets |
| title_fullStr | Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets |
| title_full_unstemmed | Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets |
| title_short | Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets |
| title_sort | growth fat metabolism and hepatic health in largemouth bass fed varying fat level diets |
| topic | Growth fat metabolism hepatic health fat levels largemouth bass |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006239 |
| work_keys_str_mv | AT digenqin growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT jialechen growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT junxiongli growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT zhiyouliu growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT weibinhuang growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT yuanzhiyang growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT jingzhang growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT beipingtan growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets AT xiaohuidong growthfatmetabolismandhepatichealthinlargemouthbassfedvaryingfatleveldiets |