Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method
Among the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Çanakkale Onsekiz Mart University
2024-09-01
|
| Series: | Journal of Advanced Research in Natural and Applied Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/3500719 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832095409771642880 |
|---|---|
| author | Ferda Dondurmacıoğlu |
| author_facet | Ferda Dondurmacıoğlu |
| author_sort | Ferda Dondurmacıoğlu |
| collection | DOAJ |
| description | Among the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate and phenazine methosulfate (NADH-PMS) system with N, N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) to form the pink colored DMPD-quinone (DMPDQ) radical. In the presence of scavengers with superoxide radical scavenging activity (antioxidants, herbal teas) the color intensity decreases due to reduced DMPDQ radical production. The absorbance of the colored reference solution and the sample solution containing the radical scavenger was measured at 552 nm. The difference in absorbance (ΔA) between the reference solution and the sample solution was found. ΔA is proportional to the scavenger concentration. In the study, the superoxide radical scavenging effect of trolox (TR) and different AOXs was investigated. The superoxide radical scavenging effect of three different herbal tea infusion solutions was measured with this method. From the graph drawn between herbal tea concentrations and percentage inhibition values, 50% inhibition (EC50) values of herbal teas were found. EC50 method values were compared with the EC50 values of the nitroblue tetrazolium (NBT) and the 2,2'-azino-bis(3-etilbenzotiyazolin-6-sülfonik asit (ABTS) method. In addition, ABTS, cupric reducing antioxidant capacity (CUPRAC), and this study total antioxidant capacity (TAC) values of herbal tea infusions were calculated and compared. |
| format | Article |
| id | doaj-art-ada2c53cea4643099e326f36c971f938 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-ada2c53cea4643099e326f36c971f9382025-02-05T18:13:02ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952024-09-0110350651910.28979/jarnas.1382201453Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD MethodFerda Dondurmacıoğlu0https://orcid.org/0000-0002-1261-8866İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞAAmong the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate and phenazine methosulfate (NADH-PMS) system with N, N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) to form the pink colored DMPD-quinone (DMPDQ) radical. In the presence of scavengers with superoxide radical scavenging activity (antioxidants, herbal teas) the color intensity decreases due to reduced DMPDQ radical production. The absorbance of the colored reference solution and the sample solution containing the radical scavenger was measured at 552 nm. The difference in absorbance (ΔA) between the reference solution and the sample solution was found. ΔA is proportional to the scavenger concentration. In the study, the superoxide radical scavenging effect of trolox (TR) and different AOXs was investigated. The superoxide radical scavenging effect of three different herbal tea infusion solutions was measured with this method. From the graph drawn between herbal tea concentrations and percentage inhibition values, 50% inhibition (EC50) values of herbal teas were found. EC50 method values were compared with the EC50 values of the nitroblue tetrazolium (NBT) and the 2,2'-azino-bis(3-etilbenzotiyazolin-6-sülfonik asit (ABTS) method. In addition, ABTS, cupric reducing antioxidant capacity (CUPRAC), and this study total antioxidant capacity (TAC) values of herbal tea infusions were calculated and compared.https://dergipark.org.tr/en/download/article-file/3500719antioxidant capacitynadh-pmsn n-dimethyl-p-phenylenediamine dihydrochloridesuperoxide radicalpercentage inhibitionantioksidan kapasitenadh-pmsn n-dimetil-p-fenilendiamin dihidroklorürsüperoksit radikaliyüzde inhibisyon |
| spellingShingle | Ferda Dondurmacıoğlu Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method Journal of Advanced Research in Natural and Applied Sciences antioxidant capacity nadh-pms n n-dimethyl-p-phenylenediamine dihydrochloride superoxide radical percentage inhibition antioksidan kapasite nadh-pms n n-dimetil-p-fenilendiamin dihidroklorür süperoksit radikali yüzde inhibisyon |
| title | Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method |
| title_full | Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method |
| title_fullStr | Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method |
| title_full_unstemmed | Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method |
| title_short | Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method |
| title_sort | determination of superoxide radical scavenging activity and total antioxidant capacity by modified dmpd method |
| topic | antioxidant capacity nadh-pms n n-dimethyl-p-phenylenediamine dihydrochloride superoxide radical percentage inhibition antioksidan kapasite nadh-pms n n-dimetil-p-fenilendiamin dihidroklorür süperoksit radikali yüzde inhibisyon |
| url | https://dergipark.org.tr/en/download/article-file/3500719 |
| work_keys_str_mv | AT ferdadondurmacıoglu determinationofsuperoxideradicalscavengingactivityandtotalantioxidantcapacitybymodifieddmpdmethod |