LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction
An accurate, highly sensitive, and precise method for quantitative analysis of tramadol (TMD) and gabapentin (GBP) by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using venlafaxine and pregabalin as internal standards (IS...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/1605950 |
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| author | Pappula Nagaraju Balaji Kodali Peda Varma Datla Surya Prakasarao Kovvasu |
| author_facet | Pappula Nagaraju Balaji Kodali Peda Varma Datla Surya Prakasarao Kovvasu |
| author_sort | Pappula Nagaraju |
| collection | DOAJ |
| description | An accurate, highly sensitive, and precise method for quantitative analysis of tramadol (TMD) and gabapentin (GBP) by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using venlafaxine and pregabalin as internal standards (ISTDs), respectively. An aliquot of 200 μL of plasma was mixed with internal standard dilution and extraction was performed by using solid phase extraction (SPE) technique. Peak resolution was achieved on Phenomenex PFP column (50×4.6 mm, 2.6 μm). The total analytical run time was 3.8 min. Both analytes were monitored using multiple reaction monitoring (MRM) scan and the mass spectrometer was operated in positive polarity mode. The method was validated for specificity, sensitivity, precision, accuracy, and other analytical parameters. The results found were satisfactory over the linear calibration range of 1-500 ng/mL and 10-6000 ng/mL for TMD and GBP, respectively. The developed method can be ready to use by scientific community for quantification of analytes in plasma samples from various clinical studies of different dose strengths. |
| format | Article |
| id | doaj-art-ad31dd8decb8437b82d6a59b1eb5ed50 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-ad31dd8decb8437b82d6a59b1eb5ed502025-02-03T01:10:09ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/16059501605950LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase ExtractionPappula Nagaraju0Balaji Kodali1Peda Varma Datla2Surya Prakasarao Kovvasu3Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur 522002, Andhra Pradesh, IndiaCollege of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar 522510, Guntur, Andhra Pradesh, IndiaClinical Pharmacology and Bio Sciences Division, RA Chem Pharma, Hyderabad, IndiaCollege of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, USAAn accurate, highly sensitive, and precise method for quantitative analysis of tramadol (TMD) and gabapentin (GBP) by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using venlafaxine and pregabalin as internal standards (ISTDs), respectively. An aliquot of 200 μL of plasma was mixed with internal standard dilution and extraction was performed by using solid phase extraction (SPE) technique. Peak resolution was achieved on Phenomenex PFP column (50×4.6 mm, 2.6 μm). The total analytical run time was 3.8 min. Both analytes were monitored using multiple reaction monitoring (MRM) scan and the mass spectrometer was operated in positive polarity mode. The method was validated for specificity, sensitivity, precision, accuracy, and other analytical parameters. The results found were satisfactory over the linear calibration range of 1-500 ng/mL and 10-6000 ng/mL for TMD and GBP, respectively. The developed method can be ready to use by scientific community for quantification of analytes in plasma samples from various clinical studies of different dose strengths.http://dx.doi.org/10.1155/2018/1605950 |
| spellingShingle | Pappula Nagaraju Balaji Kodali Peda Varma Datla Surya Prakasarao Kovvasu LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction International Journal of Analytical Chemistry |
| title | LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction |
| title_full | LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction |
| title_fullStr | LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction |
| title_full_unstemmed | LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction |
| title_short | LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction |
| title_sort | lc ms ms quantification of tramadol and gabapentin utilizing solid phase extraction |
| url | http://dx.doi.org/10.1155/2018/1605950 |
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