Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes

Refractory infections caused by multidrug-resistant bacteria pose a significant threat to public health. Here we report that ibuprofen inhibits conjugation of the RP4 plasmid and plasmids from clinical strains carrying different resistance genes including mcr-1, blaNDM, blaKPC, tet(X4), and tmexCD1-...

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Main Authors: Guangfen Zhang, Chunli Li, Xiaofan Li, Yuanyuan Li, Yunbing Li, Xiangkun Zeng, Chen Xu, Shuyan Wu, Ning Dong
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Journal of Global Antimicrobial Resistance
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213716525000153
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author Guangfen Zhang
Chunli Li
Xiaofan Li
Yuanyuan Li
Yunbing Li
Xiangkun Zeng
Chen Xu
Shuyan Wu
Ning Dong
author_facet Guangfen Zhang
Chunli Li
Xiaofan Li
Yuanyuan Li
Yunbing Li
Xiangkun Zeng
Chen Xu
Shuyan Wu
Ning Dong
author_sort Guangfen Zhang
collection DOAJ
description Refractory infections caused by multidrug-resistant bacteria pose a significant threat to public health. Here we report that ibuprofen inhibits conjugation of the RP4 plasmid and plasmids from clinical strains carrying different resistance genes including mcr-1, blaNDM, blaKPC, tet(X4), and tmexCD1-toprJ1. Mechanistic studies suggest that ibuprofen reduces ATP production and inhibits conjugation-related genes. The inhibitory effect of ibuprofen on conjugation has significant clinical implications for preventing the spread of multidrug resistance, opening new therapeutic avenues to combat multidrug-resistant bacteria.
format Article
id doaj-art-acdc9a4d70b34a6fbb0a30df1a7f89a2
institution OA Journals
issn 2213-7165
language English
publishDate 2025-05-01
publisher Elsevier
record_format Article
series Journal of Global Antimicrobial Resistance
spelling doaj-art-acdc9a4d70b34a6fbb0a30df1a7f89a22025-08-20T02:14:22ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-05-0142101410.1016/j.jgar.2025.01.012Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genesGuangfen Zhang0Chunli Li1Xiaofan Li2Yuanyuan Li3Yunbing Li4Xiangkun Zeng5Chen Xu6Shuyan Wu7Ning Dong8MOE Key Laboratory of Geriatric Diseases & Immunology, Suzhou Key Laboratory of Pathogen Bioscience & Anti-infective Medicine, Department of Medical Microbiology, School of Basic Medical Science, Suzhou Medical College, Soochow University, Suzhou, China; School of Public Health, Zhejiang University School of Medicine, Hangzhou, ChinaMOE Key Laboratory of Geriatric Diseases & Immunology, Suzhou Key Laboratory of Pathogen Bioscience & Anti-infective Medicine, Department of Medical Microbiology, School of Basic Medical Science, Suzhou Medical College, Soochow University, Suzhou, China; School of Public Health, Zhejiang University School of Medicine, Hangzhou, ChinaSchool of Public Health, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Medical Microbiology, Experimental Center, Medical College of Soochow University, Suzhou, ChinaDepartment of Medical Microbiology, Experimental Center, Medical College of Soochow University, Suzhou, ChinaMOE Key Laboratory of Geriatric Diseases & Immunology, Suzhou Key Laboratory of Pathogen Bioscience & Anti-infective Medicine, Department of Medical Microbiology, School of Basic Medical Science, Suzhou Medical College, Soochow University, Suzhou, China; School of Public Health, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, ChinaMOE Key Laboratory of Geriatric Diseases & Immunology, Suzhou Key Laboratory of Pathogen Bioscience & Anti-infective Medicine, Department of Medical Microbiology, School of Basic Medical Science, Suzhou Medical College, Soochow University, Suzhou, ChinaMOE Key Laboratory of Geriatric Diseases & Immunology, Suzhou Key Laboratory of Pathogen Bioscience & Anti-infective Medicine, Department of Medical Microbiology, School of Basic Medical Science, Suzhou Medical College, Soochow University, Suzhou, China; School of Public Health, Zhejiang University School of Medicine, Hangzhou, China; Corresponding author. Department of Emergency Medicine, Second Affiliated Hospital, Department of Epidemiology and Biostatistics, School of Public Health, The Key Laboratory of Intelligent Preventive Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.Refractory infections caused by multidrug-resistant bacteria pose a significant threat to public health. Here we report that ibuprofen inhibits conjugation of the RP4 plasmid and plasmids from clinical strains carrying different resistance genes including mcr-1, blaNDM, blaKPC, tet(X4), and tmexCD1-toprJ1. Mechanistic studies suggest that ibuprofen reduces ATP production and inhibits conjugation-related genes. The inhibitory effect of ibuprofen on conjugation has significant clinical implications for preventing the spread of multidrug resistance, opening new therapeutic avenues to combat multidrug-resistant bacteria.http://www.sciencedirect.com/science/article/pii/S2213716525000153IbuprofenConjugative transferAntimicrobial resistanceType IV secretion systemATP level
spellingShingle Guangfen Zhang
Chunli Li
Xiaofan Li
Yuanyuan Li
Yunbing Li
Xiangkun Zeng
Chen Xu
Shuyan Wu
Ning Dong
Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
Journal of Global Antimicrobial Resistance
Ibuprofen
Conjugative transfer
Antimicrobial resistance
Type IV secretion system
ATP level
title Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
title_full Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
title_fullStr Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
title_full_unstemmed Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
title_short Ibuprofen prevents the conjugative transfer of plasmid-mediated antimicrobial resistance genes
title_sort ibuprofen prevents the conjugative transfer of plasmid mediated antimicrobial resistance genes
topic Ibuprofen
Conjugative transfer
Antimicrobial resistance
Type IV secretion system
ATP level
url http://www.sciencedirect.com/science/article/pii/S2213716525000153
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