Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice
The cytotoxicity and biosafety of poly-(3-hydroxybutyrate) (P3HB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV) films were investigated in vitro using 3T3 fibroblast cells and in vivo through subcutaneous implantation of the film in mice. The in vitro test revealed that endotoxin-free P3...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2014/789681 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556289841954816 |
|---|---|
| author | Yun Chen Ya-Hui Tsai I-Ning Chou Sheng-Hong Tseng Ho-Shing Wu |
| author_facet | Yun Chen Ya-Hui Tsai I-Ning Chou Sheng-Hong Tseng Ho-Shing Wu |
| author_sort | Yun Chen |
| collection | DOAJ |
| description | The cytotoxicity and biosafety of poly-(3-hydroxybutyrate) (P3HB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV) films were investigated in vitro using 3T3 fibroblast cells and in vivo through subcutaneous implantation of the film in mice. The in vitro test revealed that endotoxin-free P3HB and P3HBV films allowed cell attachment and growth. Film-soaked conditional media showed no significant inhibitory or cytotoxic effects on cell proliferation. The in vivo absorption test showed that both the P3HB and P3HBV films slowly degraded and that P3HB had a slower degradation rate than that of P3HBV. Applying a P3HB film in hernia repair demonstrated a favorable outcome: the film was able to correct the abdominal ventral hernia by inducing connective tissue and fat ingrowth and exhibited an extremely slow rate of degradation. Furthermore, the P3HB film demonstrated the advantage of lower intestinal adhesion to the ventral hernia site compared with the P3HBV and PP commercial films. |
| format | Article |
| id | doaj-art-abd51f1fc7e748fb82829317785421f7 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-abd51f1fc7e748fb82829317785421f72025-02-03T05:45:55ZengWileyInternational Journal of Polymer Science1687-94221687-94302014-01-01201410.1155/2014/789681789681Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in MiceYun Chen0Ya-Hui Tsai1I-Ning Chou2Sheng-Hong Tseng3Ho-Shing Wu4Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, TaiwanDepartment of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, TaiwanDepartment of Chemical Engineering and Materials Science, Yuan Ze University, 135 Yuan Tung Road, Chung-Li, Taoyuan 32003, TaiwanDepartment of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 100, TaiwanDepartment of Chemical Engineering and Materials Science, Yuan Ze University, 135 Yuan Tung Road, Chung-Li, Taoyuan 32003, TaiwanThe cytotoxicity and biosafety of poly-(3-hydroxybutyrate) (P3HB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV) films were investigated in vitro using 3T3 fibroblast cells and in vivo through subcutaneous implantation of the film in mice. The in vitro test revealed that endotoxin-free P3HB and P3HBV films allowed cell attachment and growth. Film-soaked conditional media showed no significant inhibitory or cytotoxic effects on cell proliferation. The in vivo absorption test showed that both the P3HB and P3HBV films slowly degraded and that P3HB had a slower degradation rate than that of P3HBV. Applying a P3HB film in hernia repair demonstrated a favorable outcome: the film was able to correct the abdominal ventral hernia by inducing connective tissue and fat ingrowth and exhibited an extremely slow rate of degradation. Furthermore, the P3HB film demonstrated the advantage of lower intestinal adhesion to the ventral hernia site compared with the P3HBV and PP commercial films.http://dx.doi.org/10.1155/2014/789681 |
| spellingShingle | Yun Chen Ya-Hui Tsai I-Ning Chou Sheng-Hong Tseng Ho-Shing Wu Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice International Journal of Polymer Science |
| title | Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice |
| title_full | Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice |
| title_fullStr | Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice |
| title_full_unstemmed | Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice |
| title_short | Application of Biodegradable Polyhydroxyalkanoates as Surgical Films for Ventral Hernia Repair in Mice |
| title_sort | application of biodegradable polyhydroxyalkanoates as surgical films for ventral hernia repair in mice |
| url | http://dx.doi.org/10.1155/2014/789681 |
| work_keys_str_mv | AT yunchen applicationofbiodegradablepolyhydroxyalkanoatesassurgicalfilmsforventralherniarepairinmice AT yahuitsai applicationofbiodegradablepolyhydroxyalkanoatesassurgicalfilmsforventralherniarepairinmice AT iningchou applicationofbiodegradablepolyhydroxyalkanoatesassurgicalfilmsforventralherniarepairinmice AT shenghongtseng applicationofbiodegradablepolyhydroxyalkanoatesassurgicalfilmsforventralherniarepairinmice AT hoshingwu applicationofbiodegradablepolyhydroxyalkanoatesassurgicalfilmsforventralherniarepairinmice |