Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis)
First generation (F1) of hatchery produced humpback grouper (Cromileptes altivelis) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at...
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Asosiasi Sains Akuakultur Indonesia
2007-01-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/3989 |
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| author | Ratu Siti Aliah . Wahidah K. Sumantadinata Estu Nugroho O. Carman |
| author_facet | Ratu Siti Aliah . Wahidah K. Sumantadinata Estu Nugroho O. Carman |
| author_sort | Ratu Siti Aliah |
| collection | DOAJ |
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First generation (F1) of hatchery produced humpback grouper (Cromileptes altivelis) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at BBPBL Lampung and BBAP Situbondo. The result of study showed that reducing of haplotype diversity had been arised from broodstock (0.8548) to F1 generation population (0.7473; 0.7273; and 0.6947, respectively). Genetic divergence that had found between population BBPBL Lampung and BBAP Situbondo make it possible to do outbreeding in order to get its heterosis's effect.
Keywords: mtDNA, haplotype diversity, genetic differentiation, Cromileptes altivelis
ABSTRAK
Ikan kerapu tikus (Cromileptes altivelis) generasi pertama (F1) hasil domestikasi di hatchery telah dikarakterisasi secara genetik untuk menyediakan informasi status sehubungan dengan program pemuliaannya. Metode PCR-RFLP digunakan untuk mendeteksi variasi sekuens D-loop mtDNA ikan kerapu tikus F1 yang diproduksi di BBPBL Lampung dan BBAP Situbondo. Hasil penelitian menunjukkan bahwa telah terjadi penurunan keragaman haplotipe dari induk (0,8548) ke populasi generasi F1 (masing-masing 0,7473; 0,7273; dan 0,6947). Adanya keragaman genetik antara populasi ikan kerapu tikus di BBPBL dan BBAP Situbondo memungkinkan dilakukannya outbreeding untuk mendapatkan efek heterosis.
Kata kunci: mtDNA, keragaman haplotipe, diferensiasi genetik, Cromileptes altivelis
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| format | Article |
| id | doaj-art-abd498b3ebde405a9c157746d0a99b5a |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-abd498b3ebde405a9c157746d0a99b5a2025-01-24T18:05:20ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-01-015110.19027/jai.5.87-963599Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis)Ratu Siti Aliah0. Wahidah1K. Sumantadinata2Estu Nugroho3O. Carman4Pusat Teknologi Pertanian, BPP TeknologiPoliteknik Pertanian Negeri Pangkajene Kepulauan, Sulawesi SelatanBogor Agricultural University, Department of AquacultureBadan Riset Kelautan dan Perikanan, DKPBogor Agricultural University, Department of Aquaculture First generation (F1) of hatchery produced humpback grouper (Cromileptes altivelis) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at BBPBL Lampung and BBAP Situbondo. The result of study showed that reducing of haplotype diversity had been arised from broodstock (0.8548) to F1 generation population (0.7473; 0.7273; and 0.6947, respectively). Genetic divergence that had found between population BBPBL Lampung and BBAP Situbondo make it possible to do outbreeding in order to get its heterosis's effect. Keywords: mtDNA, haplotype diversity, genetic differentiation, Cromileptes altivelis ABSTRAK Ikan kerapu tikus (Cromileptes altivelis) generasi pertama (F1) hasil domestikasi di hatchery telah dikarakterisasi secara genetik untuk menyediakan informasi status sehubungan dengan program pemuliaannya. Metode PCR-RFLP digunakan untuk mendeteksi variasi sekuens D-loop mtDNA ikan kerapu tikus F1 yang diproduksi di BBPBL Lampung dan BBAP Situbondo. Hasil penelitian menunjukkan bahwa telah terjadi penurunan keragaman haplotipe dari induk (0,8548) ke populasi generasi F1 (masing-masing 0,7473; 0,7273; dan 0,6947). Adanya keragaman genetik antara populasi ikan kerapu tikus di BBPBL dan BBAP Situbondo memungkinkan dilakukannya outbreeding untuk mendapatkan efek heterosis. Kata kunci: mtDNA, keragaman haplotipe, diferensiasi genetik, Cromileptes altivelis https://journal.ipb.ac.id/index.php/jai/article/view/3989 |
| spellingShingle | Ratu Siti Aliah . Wahidah K. Sumantadinata Estu Nugroho O. Carman Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) Jurnal Akuakultur Indonesia |
| title | Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) |
| title_full | Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) |
| title_fullStr | Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) |
| title_full_unstemmed | Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) |
| title_short | Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) |
| title_sort | genetic characterization of domesticated f1 generation in humpback grouper cromileptes altivelis |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/3989 |
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