Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production
Abstract The α-amylase from Cucurbita moschata Duchesne seeds (CmAmy) was purified using ammonium sulfate fractionation, followed by β-cyclodextrin sepharose 6B affinity chromatography. The enzyme exhibited a specific activity of 475.00 U/mg, resulting in a 208.54-fold and a 30.89% yield. The optima...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-11653-z |
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