Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation
Patients with chronic viral hepatitis display increased expression of Foxp3 in liver, suggesting that Tregs expansion contributes to persistent infection. The purpose of this study was to elucidate whether the expression of Foxp3 relates not to the viral infection but to the resulting liver inflamma...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/827565 |
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| author | Matthaios Speletas Nikoletta Argentou Georgios Germanidis Themistoclis Vasiliadis Konstantinos Mantzoukis Kalliopi Patsiaoura Pavlos Nikolaidis Vaios Karanikas Konstantinos Ritis Anastasios E. Germenis |
| author_facet | Matthaios Speletas Nikoletta Argentou Georgios Germanidis Themistoclis Vasiliadis Konstantinos Mantzoukis Kalliopi Patsiaoura Pavlos Nikolaidis Vaios Karanikas Konstantinos Ritis Anastasios E. Germenis |
| author_sort | Matthaios Speletas |
| collection | DOAJ |
| description | Patients with chronic viral hepatitis display increased expression of Foxp3 in liver, suggesting that Tregs expansion contributes to persistent infection. The purpose of this study was to elucidate whether the expression of Foxp3 relates not to the viral infection but to the resulting liver inflammation. Liver biopsies obtained from 69 individuals (26 chronic HBV hepatitis, 14 chronic HCV hepatitis, 11 nonalcoholic fatty liver disease, 8 autoimmune diseases, 2 methotrexate-related toxicity, and 8 controls) were examined, by qRT-PCR, for the mRNA expression of Foxp3, IL-10, TGF-β1, Fas, FasL, TRAIL, caspase-3, TNF-α, IFN-γ, and IL-1β. Significant increase of Foxp3 was observed in all disease groups compared to controls, which was positively correlated with the intensity of inflammation. The expression of the apoptosis mediators Fas, FasL, and TRAIL, but not of IL-10 and TGF-β1, was also significantly elevated. Our findings indicate that, independently of the initial inducer, liver inflammation is correlated with elevated expression of apoptosis mediators and is followed by local Treg accumulation. Further research towards the elucidation of the underlying casual relationships is required, in order to clarify whether our results signify the existence of a uniform Treg-mediated regulatory mechanism of apoptosis-induced inflammation. |
| format | Article |
| id | doaj-art-ab48d5aa37634431837b98ede6f0b32f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ab48d5aa37634431837b98ede6f0b32f2025-02-03T01:33:14ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/827565827565Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of InflammationMatthaios Speletas0Nikoletta Argentou1Georgios Germanidis2Themistoclis Vasiliadis3Konstantinos Mantzoukis4Kalliopi Patsiaoura5Pavlos Nikolaidis6Vaios Karanikas7Konstantinos Ritis8Anastasios E. Germenis9Department of Immunology and Histocompatibility, Medical School, University of Thessaly, Biopolis 41110 Larissa, GreeceDepartment of Immunology and Histocompatibility, Medical School, University of Thessaly, Biopolis 41110 Larissa, GreeceFirst Department of Internal Medicine, AHEPA Hospital, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceGastroenterology and Hepatology Division, Hippokration Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, GreeceFirst Department of Internal Medicine, AHEPA Hospital, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceDepartment of Pathology, Hippokration Hospital, 54635 Thessaloniki, GreeceFirst Department of Internal Medicine, AHEPA Hospital, Aristotle University of Thessaloniki, 54636 Thessaloniki, GreeceDepartment of Immunology and Histocompatibility, Medical School, University of Thessaly, Biopolis 41110 Larissa, GreeceFirst Department of Internal Medicine, Medical School, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Immunology and Histocompatibility, Medical School, University of Thessaly, Biopolis 41110 Larissa, GreecePatients with chronic viral hepatitis display increased expression of Foxp3 in liver, suggesting that Tregs expansion contributes to persistent infection. The purpose of this study was to elucidate whether the expression of Foxp3 relates not to the viral infection but to the resulting liver inflammation. Liver biopsies obtained from 69 individuals (26 chronic HBV hepatitis, 14 chronic HCV hepatitis, 11 nonalcoholic fatty liver disease, 8 autoimmune diseases, 2 methotrexate-related toxicity, and 8 controls) were examined, by qRT-PCR, for the mRNA expression of Foxp3, IL-10, TGF-β1, Fas, FasL, TRAIL, caspase-3, TNF-α, IFN-γ, and IL-1β. Significant increase of Foxp3 was observed in all disease groups compared to controls, which was positively correlated with the intensity of inflammation. The expression of the apoptosis mediators Fas, FasL, and TRAIL, but not of IL-10 and TGF-β1, was also significantly elevated. Our findings indicate that, independently of the initial inducer, liver inflammation is correlated with elevated expression of apoptosis mediators and is followed by local Treg accumulation. Further research towards the elucidation of the underlying casual relationships is required, in order to clarify whether our results signify the existence of a uniform Treg-mediated regulatory mechanism of apoptosis-induced inflammation.http://dx.doi.org/10.1155/2011/827565 |
| spellingShingle | Matthaios Speletas Nikoletta Argentou Georgios Germanidis Themistoclis Vasiliadis Konstantinos Mantzoukis Kalliopi Patsiaoura Pavlos Nikolaidis Vaios Karanikas Konstantinos Ritis Anastasios E. Germenis Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation Mediators of Inflammation |
| title | Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation |
| title_full | Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation |
| title_fullStr | Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation |
| title_full_unstemmed | Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation |
| title_short | Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation |
| title_sort | foxp3 expression in liver correlates with the degree but not the cause of inflammation |
| url | http://dx.doi.org/10.1155/2011/827565 |
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