In situ-formed cryomicroneedles for intradermal cell delivery
Abstract Cryomicroneedles (cryoMNs) offer a convenient and minimally invasive way to precisely deliver therapeutic cells intradermally for treating local and systemic diseases. cryoMNs are manufactured by shaping and freezing the cell-containing cryogenic media in a microneedle template, which allow...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | NPG Asia Materials |
| Online Access: | https://doi.org/10.1038/s41427-024-00531-1 |
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| author | Mengjia Zheng Tianli Hu Yating Yang Xuan Qie Huaxin Yang Yuyue Zhang Qizheng Zhang Ken-Tye Yong Wei Liu Chenjie Xu |
| author_facet | Mengjia Zheng Tianli Hu Yating Yang Xuan Qie Huaxin Yang Yuyue Zhang Qizheng Zhang Ken-Tye Yong Wei Liu Chenjie Xu |
| author_sort | Mengjia Zheng |
| collection | DOAJ |
| description | Abstract Cryomicroneedles (cryoMNs) offer a convenient and minimally invasive way to precisely deliver therapeutic cells intradermally for treating local and systemic diseases. cryoMNs are manufactured by shaping and freezing the cell-containing cryogenic media in a microneedle template, which allows cells to be packaged in advance for direct usage in the clinic. However, the current cryoMNs require cold-chain transportation and storage and do not permit the loading of autologous cells in situ. This article introduces the second generation of cryoMNs (S-cryoMNs) that address these limitations. Specifically, S-cryoMNs are made by dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature, and researchers can load any cells with the optimized cryogenic medium. As a proof-of-concept, we examined the loading and intradermal delivery of three cell types in clinically relevant in vitro and in vivo models, including mesenchymal stem cells for wound healing, melanocytes for vitiligo treatment, and antigen-pulsed dendritic cells for cancer vaccination. |
| format | Article |
| id | doaj-art-ab22d74124bf4e358ab2dbd69b52ccd6 |
| institution | Kabale University |
| issn | 1884-4057 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | NPG Asia Materials |
| spelling | doaj-art-ab22d74124bf4e358ab2dbd69b52ccd62025-01-19T12:28:43ZengNature PortfolioNPG Asia Materials1884-40572024-02-0116111410.1038/s41427-024-00531-1In situ-formed cryomicroneedles for intradermal cell deliveryMengjia Zheng0Tianli Hu1Yating Yang2Xuan Qie3Huaxin Yang4Yuyue Zhang5Qizheng Zhang6Ken-Tye Yong7Wei Liu8Chenjie Xu9Department of Biomedical Engineering, City University of Hong KongDepartment of Biomedical Engineering, City University of Hong KongDepartment of Plastic and Reconstructive Surgery, Shanghai Tissue Engineering Key Laboratory, Shanghai Research Institute of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Biomedical Engineering, City University of Hong KongDepartment of Biomedical Engineering, City University of Hong KongDepartment of Biomedical Engineering, City University of Hong KongDepartment of Biomedical Engineering, City University of Hong KongSchool of Biomedical Engineering, The University of SydneyDepartment of Plastic and Reconstructive Surgery, Shanghai Tissue Engineering Key Laboratory, Shanghai Research Institute of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Biomedical Engineering, City University of Hong KongAbstract Cryomicroneedles (cryoMNs) offer a convenient and minimally invasive way to precisely deliver therapeutic cells intradermally for treating local and systemic diseases. cryoMNs are manufactured by shaping and freezing the cell-containing cryogenic media in a microneedle template, which allows cells to be packaged in advance for direct usage in the clinic. However, the current cryoMNs require cold-chain transportation and storage and do not permit the loading of autologous cells in situ. This article introduces the second generation of cryoMNs (S-cryoMNs) that address these limitations. Specifically, S-cryoMNs are made by dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature, and researchers can load any cells with the optimized cryogenic medium. As a proof-of-concept, we examined the loading and intradermal delivery of three cell types in clinically relevant in vitro and in vivo models, including mesenchymal stem cells for wound healing, melanocytes for vitiligo treatment, and antigen-pulsed dendritic cells for cancer vaccination.https://doi.org/10.1038/s41427-024-00531-1 |
| spellingShingle | Mengjia Zheng Tianli Hu Yating Yang Xuan Qie Huaxin Yang Yuyue Zhang Qizheng Zhang Ken-Tye Yong Wei Liu Chenjie Xu In situ-formed cryomicroneedles for intradermal cell delivery NPG Asia Materials |
| title | In situ-formed cryomicroneedles for intradermal cell delivery |
| title_full | In situ-formed cryomicroneedles for intradermal cell delivery |
| title_fullStr | In situ-formed cryomicroneedles for intradermal cell delivery |
| title_full_unstemmed | In situ-formed cryomicroneedles for intradermal cell delivery |
| title_short | In situ-formed cryomicroneedles for intradermal cell delivery |
| title_sort | in situ formed cryomicroneedles for intradermal cell delivery |
| url | https://doi.org/10.1038/s41427-024-00531-1 |
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