Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities
Abstract Nanotechnology has experienced significant advancements, attracting considerable attention in various biomedical applications. This innovative study synthesizes and characterizes Ge/PLA/AuNCs (gelatin/PLA/gold nanocomposites) using Syzygium cumini extract to evaluate their various biomedica...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84098-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594713599803392 |
|---|---|
| author | Manickam Rajkumar S. I. Davis Presley Nathiya Thiyagarajulu Koyeli Girigoswami Gopalarethinam Janani Chinnaperumal Kamaraj Bharathi Madheswaran Bhupendra Prajapati Nemat Ali Mohammad Rashid Khan |
| author_facet | Manickam Rajkumar S. I. Davis Presley Nathiya Thiyagarajulu Koyeli Girigoswami Gopalarethinam Janani Chinnaperumal Kamaraj Bharathi Madheswaran Bhupendra Prajapati Nemat Ali Mohammad Rashid Khan |
| author_sort | Manickam Rajkumar |
| collection | DOAJ |
| description | Abstract Nanotechnology has experienced significant advancements, attracting considerable attention in various biomedical applications. This innovative study synthesizes and characterizes Ge/PLA/AuNCs (gelatin/PLA/gold nanocomposites) using Syzygium cumini extract to evaluate their various biomedical applications. The UV–Visible spectroscopy results in an absorption peak at 534 nm were primarily confirmed by Ge/PLA/AuNCs synthesis. The FTIR spectrum showed various functional groups and the XRD patterns confirmed the crystalline shape and structure of nanocomposites. The FESEM and HRTEM results showed a oval shape of Ge/PLA/AuNCs with an average particle size of 21 nm. The Ge/PLA/AuNC’s remarkable antioxidant activity, as evidenced by DPPH (70.84 ± 1.64%), ABTS activity (86.17 ± 1.96%), and reducing power activity (78.42 ± 1.48%) at a concentration of 100 μg/mL was observed. The zone of inhibition against Staphylococcus aureus (19.45 ± 0.89 mm) and Echericia coli (20.83 ± 0.97 mm) revealed the excellent antibacterial activity of Ge/PLA/AuNCs. The anti-diabetic activity of Ge/PLA/AuNCs was supported by inhibition of α-amylase (82.56 ± 1.49%) and α-glucosidase (80.27 ± 1.57%). The anti-Alzheimer activity was confirmed by inhibition of the AChE (76.37 ± 1.18%) and BChE (85.94 ± 1.38%) enzymes. In vivo studies of zebrafish embryos showed that Ge/PLA/AuNCs have excellent biocompatibility and nontoxicity. The SH-SY5Y cell line study demonstrated improved cell viability (95.27 ± 1.62%) and enhanced neuronal cell growth following Ge/PLA/AuNCs treatment. In conclusion, the present study highlights the cost-effective and non-toxic properties of Ge/PLA/AuNCs. Furthermore, it presents an attractive and promising approach for various future biomedical applications. |
| format | Article |
| id | doaj-art-ab18221106e64dfe97a3603ca77ba78d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ab18221106e64dfe97a3603ca77ba78d2025-01-19T12:23:32ZengNature PortfolioScientific Reports2045-23222025-01-0115112010.1038/s41598-024-84098-5Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activitiesManickam Rajkumar0S. I. Davis Presley1Nathiya Thiyagarajulu2Koyeli Girigoswami3Gopalarethinam Janani4Chinnaperumal Kamaraj5Bharathi Madheswaran6Bhupendra Prajapati7Nemat Ali8Mohammad Rashid Khan9Department of Chemistry, Sri Sivasubramaniya Nadar College of EngineeringDepartment of Chemistry, Sri Sivasubramaniya Nadar College of EngineeringDepartment of Life Sciences, Kristu Jayanti CollegeFaculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and EducationFaculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and EducationInterdisciplinary Institute of Indian System of Medicine (IIISM), Directorate of Research, SRM Institute Science and Technology, KattankulathurDepartment of Pharmaceutical Engineering, Vinayaka Mission’s Kirupananda Variyar Engineering College, AriyanurShree S. K. Patel College of Pharmaceutical Education and Research, Ganpat UniversityDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud UniversityDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud UniversityAbstract Nanotechnology has experienced significant advancements, attracting considerable attention in various biomedical applications. This innovative study synthesizes and characterizes Ge/PLA/AuNCs (gelatin/PLA/gold nanocomposites) using Syzygium cumini extract to evaluate their various biomedical applications. The UV–Visible spectroscopy results in an absorption peak at 534 nm were primarily confirmed by Ge/PLA/AuNCs synthesis. The FTIR spectrum showed various functional groups and the XRD patterns confirmed the crystalline shape and structure of nanocomposites. The FESEM and HRTEM results showed a oval shape of Ge/PLA/AuNCs with an average particle size of 21 nm. The Ge/PLA/AuNC’s remarkable antioxidant activity, as evidenced by DPPH (70.84 ± 1.64%), ABTS activity (86.17 ± 1.96%), and reducing power activity (78.42 ± 1.48%) at a concentration of 100 μg/mL was observed. The zone of inhibition against Staphylococcus aureus (19.45 ± 0.89 mm) and Echericia coli (20.83 ± 0.97 mm) revealed the excellent antibacterial activity of Ge/PLA/AuNCs. The anti-diabetic activity of Ge/PLA/AuNCs was supported by inhibition of α-amylase (82.56 ± 1.49%) and α-glucosidase (80.27 ± 1.57%). The anti-Alzheimer activity was confirmed by inhibition of the AChE (76.37 ± 1.18%) and BChE (85.94 ± 1.38%) enzymes. In vivo studies of zebrafish embryos showed that Ge/PLA/AuNCs have excellent biocompatibility and nontoxicity. The SH-SY5Y cell line study demonstrated improved cell viability (95.27 ± 1.62%) and enhanced neuronal cell growth following Ge/PLA/AuNCs treatment. In conclusion, the present study highlights the cost-effective and non-toxic properties of Ge/PLA/AuNCs. Furthermore, it presents an attractive and promising approach for various future biomedical applications.https://doi.org/10.1038/s41598-024-84098-5NanocompositeSyzygium cuminiAntioxidantsAnti-diabeticAnti-Alzheimer’s |
| spellingShingle | Manickam Rajkumar S. I. Davis Presley Nathiya Thiyagarajulu Koyeli Girigoswami Gopalarethinam Janani Chinnaperumal Kamaraj Bharathi Madheswaran Bhupendra Prajapati Nemat Ali Mohammad Rashid Khan Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities Scientific Reports Nanocomposite Syzygium cumini Antioxidants Anti-diabetic Anti-Alzheimer’s |
| title | Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities |
| title_full | Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities |
| title_fullStr | Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities |
| title_full_unstemmed | Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities |
| title_short | Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s activities |
| title_sort | gelatin pla loaded gold nanocomposites synthesis using syzygium cumini fruit extract and their antioxidant antibacterial anti inflammatory antidiabetic and anti alzheimer s activities |
| topic | Nanocomposite Syzygium cumini Antioxidants Anti-diabetic Anti-Alzheimer’s |
| url | https://doi.org/10.1038/s41598-024-84098-5 |
| work_keys_str_mv | AT manickamrajkumar gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT sidavispresley gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT nathiyathiyagarajulu gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT koyeligirigoswami gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT gopalarethinamjanani gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT chinnaperumalkamaraj gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT bharathimadheswaran gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT bhupendraprajapati gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT nematali gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities AT mohammadrashidkhan gelatinplaloadedgoldnanocompositessynthesisusingsyzygiumcuminifruitextractandtheirantioxidantantibacterialantiinflammatoryantidiabeticandantialzheimersactivities |