Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression
Background: The immunologic factors are the chief reason for recurrent pregnancy loss (RPL) and induction ofmaternal-fetal tolerance is the main treatment for this cause of RPL, but the effect of this method is uncertainly andneeds multiple doses and/or interventions. The aim of this study was to in...
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Royan Institute (ACECR), Tehran
2025-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | https://www.ijfs.ir/article_713784_7f727a4479a0231944890953c04c5cbe.pdf |
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| author | Xinghui Wen Xiaojuan Chen Mei Li Jinnian Zhou Shouli Dao Shixiang Li Hui Cheng Shuyun Zhao Guanyou Huang |
| author_facet | Xinghui Wen Xiaojuan Chen Mei Li Jinnian Zhou Shouli Dao Shixiang Li Hui Cheng Shuyun Zhao Guanyou Huang |
| author_sort | Xinghui Wen |
| collection | DOAJ |
| description | Background: The immunologic factors are the chief reason for recurrent pregnancy loss (RPL) and induction ofmaternal-fetal tolerance is the main treatment for this cause of RPL, but the effect of this method is uncertainly andneeds multiple doses and/or interventions. The aim of this study was to investigate whether a single administrationof transforming growth factor-β1 (TGF-β1) can improve the pregnancy outcomes of RPL mice and whether theimprovement is cause by TGF-β1 driving the expression of immune tolerance molecule indoleamine 2,3-dioxygenase(IDO).Materials and Methods: In this experimental study, 40 RPL model mice were equally divided into a control group,that received 0.01 M phosphate-buffered saline (PBS), and a treatment group, that received PBS containing 2, 20, and200 ng/ml TGF-β1 via tail vein injection. The mice were sacrificed at 13.5 days of pregnancy and the embryo resorptionrate was determined. The expression of IDO, TGF-β1, and TGF-β3 were detected in the placenta using westernblotting and immunohistochemistry techniques.Results: The expression of IDO was positively correlated with TGF-β1 in the placental tissue of RPL mice (r=0.591,P<0.001). In all treatment groups, the embryo resorption rates were significantly lower than the control group andthe expression of IDO in the placental tissue of all treatment groups was significantly higher than the control group.The expression of TGF-β1 increased gradually from, 2, 20 to 200 ng/ml in treatment groups, and the concentrationof exogenous TGF-β1 positively correlated with the expression of TGF-β1, in placental tissues in treatment groups(r=0.372, P=0.018).Conclusion: Exogenous TGF-β1 improves pregnancy outcomes in RPL mice, and the possible therapeutic mechanismis that exogenous TGF-β1 induces the persistent expression of endogenous TGF-β1 and IDO due to mutuallyinduced expression of the other. This experiment may provide a new direction and idea for the future treatment ofRPL patients. |
| format | Article |
| id | doaj-art-aaad171b154b4975af9effa6ff2f15a2 |
| institution | Kabale University |
| issn | 2008-076X 2008-0778 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
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| series | International Journal of Fertility and Sterility |
| spelling | doaj-art-aaad171b154b4975af9effa6ff2f15a22025-01-18T09:23:38ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782025-01-01191646910.22074/ijfs.2024.2011343.1541713784Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase ExpressionXinghui Wen0Xiaojuan Chen1Mei Li2Jinnian Zhou3Shouli Dao4Shixiang Li5Hui Cheng6Shuyun Zhao7Guanyou Huang8Reproductive Medicine Center, Department of Obstetrics and Gynecology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaDepartment of Hyperbaric Oxygen Chamber, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaDepartment of Obstetrics and Gynecology, The Second People's Hospital of Guiyang City, Guiyang, Guizhou Province, ChinaDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaLiupanshui Maternal and Child Health Hospital, Liupanshui, Guizhou Province, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The Second People's Hospital of Guiyang City, Guiyang, Guizhou Province, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaReproductive Medicine Center, Department of Obstetrics and Gynecology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, ChinaBackground: The immunologic factors are the chief reason for recurrent pregnancy loss (RPL) and induction ofmaternal-fetal tolerance is the main treatment for this cause of RPL, but the effect of this method is uncertainly andneeds multiple doses and/or interventions. The aim of this study was to investigate whether a single administrationof transforming growth factor-β1 (TGF-β1) can improve the pregnancy outcomes of RPL mice and whether theimprovement is cause by TGF-β1 driving the expression of immune tolerance molecule indoleamine 2,3-dioxygenase(IDO).Materials and Methods: In this experimental study, 40 RPL model mice were equally divided into a control group,that received 0.01 M phosphate-buffered saline (PBS), and a treatment group, that received PBS containing 2, 20, and200 ng/ml TGF-β1 via tail vein injection. The mice were sacrificed at 13.5 days of pregnancy and the embryo resorptionrate was determined. The expression of IDO, TGF-β1, and TGF-β3 were detected in the placenta using westernblotting and immunohistochemistry techniques.Results: The expression of IDO was positively correlated with TGF-β1 in the placental tissue of RPL mice (r=0.591,P<0.001). In all treatment groups, the embryo resorption rates were significantly lower than the control group andthe expression of IDO in the placental tissue of all treatment groups was significantly higher than the control group.The expression of TGF-β1 increased gradually from, 2, 20 to 200 ng/ml in treatment groups, and the concentrationof exogenous TGF-β1 positively correlated with the expression of TGF-β1, in placental tissues in treatment groups(r=0.372, P=0.018).Conclusion: Exogenous TGF-β1 improves pregnancy outcomes in RPL mice, and the possible therapeutic mechanismis that exogenous TGF-β1 induces the persistent expression of endogenous TGF-β1 and IDO due to mutuallyinduced expression of the other. This experiment may provide a new direction and idea for the future treatment ofRPL patients.https://www.ijfs.ir/article_713784_7f727a4479a0231944890953c04c5cbe.pdfanimal modelindoleamine 23-dioxygenaserecurrent pregnancy losstransforming growth factor-βtreatment |
| spellingShingle | Xinghui Wen Xiaojuan Chen Mei Li Jinnian Zhou Shouli Dao Shixiang Li Hui Cheng Shuyun Zhao Guanyou Huang Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression International Journal of Fertility and Sterility animal model indoleamine 2 3-dioxygenase recurrent pregnancy loss transforming growth factor-β treatment |
| title | Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression |
| title_full | Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression |
| title_fullStr | Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression |
| title_full_unstemmed | Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression |
| title_short | Effect of Exogenous of Transforming Growth Factor-β1 on Pregnancy Outcome in Mice with Recurrent Pregnancy Loss by Persistent Enhancement of Placental Tissue Indoleamine 2,3-Dioxygenase Expression |
| title_sort | effect of exogenous of transforming growth factor β1 on pregnancy outcome in mice with recurrent pregnancy loss by persistent enhancement of placental tissue indoleamine 2 3 dioxygenase expression |
| topic | animal model indoleamine 2 3-dioxygenase recurrent pregnancy loss transforming growth factor-β treatment |
| url | https://www.ijfs.ir/article_713784_7f727a4479a0231944890953c04c5cbe.pdf |
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