The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.)
Some peroxidases (PRXs) are involved in abiotic stress response. However, to the best of our knowledge, the effects of PRXs on agronomic traits including grain number per spike (GNS), spikelet number per spike (SNS) and spike length (SL) are also largely unknown. In our study, we cloned a wheat PRX...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1501029/full |
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| author | Dongtian Zang Wenjia You Yangyang Wu Pengyue Wang Zhiyu Wang Qingyun Yang Shatong Chi Peisen Su Peisen Su |
| author_facet | Dongtian Zang Wenjia You Yangyang Wu Pengyue Wang Zhiyu Wang Qingyun Yang Shatong Chi Peisen Su Peisen Su |
| author_sort | Dongtian Zang |
| collection | DOAJ |
| description | Some peroxidases (PRXs) are involved in abiotic stress response. However, to the best of our knowledge, the effects of PRXs on agronomic traits including grain number per spike (GNS), spikelet number per spike (SNS) and spike length (SL) are also largely unknown. In our study, we cloned a wheat PRX gene TaPRX-2A and identified its function in controlling GNS by generating transgenic overexpression lines. The results showed that TaPRX-2A overexpression displayed lower GNS and shorter SL, compared with the wild-type plants. RNA-seq analysis indicated alterations in various pathways including flavonoid biosynthesis, lignin biosynthesis, phytohormone signaling, as well as sucrose and starch biosynthesis. Co-expression analysis showed that transcription factors, such as bHLH, WRKY, and bZIP may be involved in the regulation of various genes associated with these pathways. Our findings provide insights into the mechanisms by which PRXs regulate agronomic traits, illustrating potential applicability in crop improvement programs. |
| format | Article |
| id | doaj-art-aa910911cfca48a18d7ea95b04edeac0 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-aa910911cfca48a18d7ea95b04edeac02025-02-06T07:09:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011510.3389/fpls.2024.15010291501029The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.)Dongtian Zang0Wenjia You1Yangyang Wu2Pengyue Wang3Zhiyu Wang4Qingyun Yang5Shatong Chi6Peisen Su7Peisen Su8College of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaCollege of Agriculture and Biology, Liaocheng University, Liaocheng, ChinaInstitute of Huanghe Studies, Liaocheng University, Liaocheng, ChinaSome peroxidases (PRXs) are involved in abiotic stress response. However, to the best of our knowledge, the effects of PRXs on agronomic traits including grain number per spike (GNS), spikelet number per spike (SNS) and spike length (SL) are also largely unknown. In our study, we cloned a wheat PRX gene TaPRX-2A and identified its function in controlling GNS by generating transgenic overexpression lines. The results showed that TaPRX-2A overexpression displayed lower GNS and shorter SL, compared with the wild-type plants. RNA-seq analysis indicated alterations in various pathways including flavonoid biosynthesis, lignin biosynthesis, phytohormone signaling, as well as sucrose and starch biosynthesis. Co-expression analysis showed that transcription factors, such as bHLH, WRKY, and bZIP may be involved in the regulation of various genes associated with these pathways. Our findings provide insights into the mechanisms by which PRXs regulate agronomic traits, illustrating potential applicability in crop improvement programs.https://www.frontiersin.org/articles/10.3389/fpls.2024.1501029/fullwheatperoxidases (PRXs)TaPRX-2Agrain number per spike (GNS)co-expression analysis |
| spellingShingle | Dongtian Zang Wenjia You Yangyang Wu Pengyue Wang Zhiyu Wang Qingyun Yang Shatong Chi Peisen Su Peisen Su The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) Frontiers in Plant Science wheat peroxidases (PRXs) TaPRX-2A grain number per spike (GNS) co-expression analysis |
| title | The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) |
| title_full | The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) |
| title_fullStr | The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) |
| title_short | The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) |
| title_sort | class iii peroxidase gene taprx 2a controls grain number per spike in common wheat triticum aestivum l |
| topic | wheat peroxidases (PRXs) TaPRX-2A grain number per spike (GNS) co-expression analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1501029/full |
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