Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota
IntroductionPolygonatum kingianum is a well-known medicinal herb with proven bioactivities; however, little is known about the effects of its polysaccharide on intestinal injuries in animals induced by lipopolysaccharide (LPS).MethodsA total of 30 Institute of Cancer Research (ICR) mice were divided...
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Frontiers Media S.A.
2025-01-01
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| author | Reng Qiu Chuangye Pan Yuxi Qin Qianfei Wei Yue Yu Ying Zhang Xuehan Xie Jianqin Li Shouhai Chen Kun Li Dalia Fouad Yi Wu Qiu Zhong |
| author_facet | Reng Qiu Chuangye Pan Yuxi Qin Qianfei Wei Yue Yu Ying Zhang Xuehan Xie Jianqin Li Shouhai Chen Kun Li Dalia Fouad Yi Wu Qiu Zhong |
| author_sort | Reng Qiu |
| collection | DOAJ |
| description | IntroductionPolygonatum kingianum is a well-known medicinal herb with proven bioactivities; however, little is known about the effects of its polysaccharide on intestinal injuries in animals induced by lipopolysaccharide (LPS).MethodsA total of 30 Institute of Cancer Research (ICR) mice were divided into control (CH), induced (MH), and treated (H) groups. Mice in group H were supplemented with 100 mg/kg Polygonatum kingianum polysaccharides, while groups C and M were treated with the same amount of normal saline by gavage for 18 days. On the 18th day animals in groups M and H were induced by LPS (10 mg/kg).ResultsThe results showed the weight of mice in group MH significantly dropped (P < 0.0001), while mice in the PK group had a higher weight (P < 0.01). Pathological analysis found that the majority of the villi in mice induced by LPS were broken and short, while PK-treated animals had longer and considerably integrated villi. The villi length in groups CH (P < 0.0001) and H (P < 0.0001) was longer than that in group M, and the value of villi length/crypt depth in group MH was smaller than that in groups CH (P < 0.0001) and H (P < 0.0001), while the crypt depth in group MH was higher than in groups CH (P < 0.0001) and H (P < 0.0001). Serum inspection showed that MAD (P < 0.05), IL-1β (P < 0.05), IL-6 (P < 0.05), and TNF-α (P < 0.01) were significantly higher in group MH, while SOD (P < 0.001), T-AOC (P < 0.01), and GSH-Px (P < 0.01) were notably higher in groups CH and H. Microbiome sequencing of mice obtained 844,477 raw and 725,469 filtered reads. There were 2,407 ASVs detected in animals, and there were 312 and 328 shared ASVs between CH and MH, and CH and H, respectively. There were 5 phyla and 20genera of remarkable bacteria found among mice groups including genera of Escherichia, Pseudomonas_E, Mailhella, Paramuribaculum, NM07-P-09, Odoribacter, Nanosyncoccus, SFM01, Onthenecus, Clostridium_Q, UBA6985, Ructibacterium, UBA946, Lachnoclostridium_B, Evtepia, CAG-269, Limivicinus, Formimonas, Dehalobacterium, Dwaynesavagella, and UBA6985. We revealed that Polygonatum kingianum polysaccharide could alleviate intestinal injuries by promoting oxidation resistance, decreasing inflammatory responses, and accommodating the intestinal microbiota of mice.DiscussionOur results suggest the possibility of developing novel therapies for intestinal diseases. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-aa6e278b976647dba676f7a0fd7efc892025-01-30T06:22:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14927101492710Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiotaReng Qiu0Chuangye Pan1Yuxi Qin2Qianfei Wei3Yue Yu4Ying Zhang5Xuehan Xie6Jianqin Li7Shouhai Chen8Kun Li9Dalia Fouad10Yi Wu11Qiu Zhong12Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang Normal University, Nanyang, Henan, ChinaHenan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang Normal University, Nanyang, Henan, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, ChinaHenan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang Normal University, Nanyang, Henan, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, ChinaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan, ChinaGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, ChinaIntroductionPolygonatum kingianum is a well-known medicinal herb with proven bioactivities; however, little is known about the effects of its polysaccharide on intestinal injuries in animals induced by lipopolysaccharide (LPS).MethodsA total of 30 Institute of Cancer Research (ICR) mice were divided into control (CH), induced (MH), and treated (H) groups. Mice in group H were supplemented with 100 mg/kg Polygonatum kingianum polysaccharides, while groups C and M were treated with the same amount of normal saline by gavage for 18 days. On the 18th day animals in groups M and H were induced by LPS (10 mg/kg).ResultsThe results showed the weight of mice in group MH significantly dropped (P < 0.0001), while mice in the PK group had a higher weight (P < 0.01). Pathological analysis found that the majority of the villi in mice induced by LPS were broken and short, while PK-treated animals had longer and considerably integrated villi. The villi length in groups CH (P < 0.0001) and H (P < 0.0001) was longer than that in group M, and the value of villi length/crypt depth in group MH was smaller than that in groups CH (P < 0.0001) and H (P < 0.0001), while the crypt depth in group MH was higher than in groups CH (P < 0.0001) and H (P < 0.0001). Serum inspection showed that MAD (P < 0.05), IL-1β (P < 0.05), IL-6 (P < 0.05), and TNF-α (P < 0.01) were significantly higher in group MH, while SOD (P < 0.001), T-AOC (P < 0.01), and GSH-Px (P < 0.01) were notably higher in groups CH and H. Microbiome sequencing of mice obtained 844,477 raw and 725,469 filtered reads. There were 2,407 ASVs detected in animals, and there were 312 and 328 shared ASVs between CH and MH, and CH and H, respectively. There were 5 phyla and 20genera of remarkable bacteria found among mice groups including genera of Escherichia, Pseudomonas_E, Mailhella, Paramuribaculum, NM07-P-09, Odoribacter, Nanosyncoccus, SFM01, Onthenecus, Clostridium_Q, UBA6985, Ructibacterium, UBA946, Lachnoclostridium_B, Evtepia, CAG-269, Limivicinus, Formimonas, Dehalobacterium, Dwaynesavagella, and UBA6985. We revealed that Polygonatum kingianum polysaccharide could alleviate intestinal injuries by promoting oxidation resistance, decreasing inflammatory responses, and accommodating the intestinal microbiota of mice.DiscussionOur results suggest the possibility of developing novel therapies for intestinal diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1492710/fullEscherichiaPseudomonas_EMailhellaParamuribaculumNM07-P-09Odoribacter |
| spellingShingle | Reng Qiu Chuangye Pan Yuxi Qin Qianfei Wei Yue Yu Ying Zhang Xuehan Xie Jianqin Li Shouhai Chen Kun Li Dalia Fouad Yi Wu Qiu Zhong Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota Frontiers in Microbiology Escherichia Pseudomonas_E Mailhella Paramuribaculum NM07-P-09 Odoribacter |
| title | Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| title_full | Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| title_fullStr | Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| title_full_unstemmed | Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| title_short | Polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| title_sort | polygonatum kingianum polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota |
| topic | Escherichia Pseudomonas_E Mailhella Paramuribaculum NM07-P-09 Odoribacter |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1492710/full |
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