DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats
Dimethylarsinic acid (DMAV) is the main product of arsenic methylation metabolism in vivo and is rat bladder carcinogen and tumor promoting agent. In this study, we measured the expressions of mRNA and proteins of NF-κB pathway members, IKKα, IKKβ, p65, and p50 in rat bladder epithelium by qRT-PCR a...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/790652 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559367152467968 |
|---|---|
| author | Siqi Cao Shengnan Liu Fei Wang Jieyu Liu Mengdan Li Chen Wang Shuhua Xi |
| author_facet | Siqi Cao Shengnan Liu Fei Wang Jieyu Liu Mengdan Li Chen Wang Shuhua Xi |
| author_sort | Siqi Cao |
| collection | DOAJ |
| description | Dimethylarsinic acid (DMAV) is the main product of arsenic methylation metabolism in vivo and is rat bladder carcinogen and tumor promoting agent. In this study, we measured the expressions of mRNA and proteins of NF-κB pathway members, IKKα, IKKβ, p65, and p50 in rat bladder epithelium by qRT-PCR and immunohistochemical analysis after rats received drinking water containing 100 and 200 ppm DMAV for 10 weeks. Transforming growth factor-β (TGF-β) immunoexpression in rat bladder epithelium and urine level of IL-1β also were determined. We found that DMAV dramatically increased the mRNA levels of NF-κB p50 and IKKα in the bladder epithelium of rats compared to the control group. Immunohistochemical examinations showed that DMAV increased immunoreactivities of IKKα, IKKβ, and phospho-NF-κB p50 in the cytoplasm and phospho-NF-κB p50 and p65 in nucleus of rat urothelial cells. In addition, DMAV treated rats exhibited significantly increased inflammatory factor TGF-β immunoreactivity in bladder epithelium and IL-1β secretion in urine. These data suggest that DMAV could activate NF-κB signal pathway and increase TGF-β and IL-1β expressions in bladder epithelial cells of rats. |
| format | Article |
| id | doaj-art-a9e3b287516449f999b74e93e584b21c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a9e3b287516449f999b74e93e584b21c2025-02-03T01:30:17ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/790652790652DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of RatsSiqi Cao0Shengnan Liu1Fei Wang2Jieyu Liu3Mengdan Li4Chen Wang5Shuhua Xi6Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDepartment of Environmental and Occupational Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, ChinaDimethylarsinic acid (DMAV) is the main product of arsenic methylation metabolism in vivo and is rat bladder carcinogen and tumor promoting agent. In this study, we measured the expressions of mRNA and proteins of NF-κB pathway members, IKKα, IKKβ, p65, and p50 in rat bladder epithelium by qRT-PCR and immunohistochemical analysis after rats received drinking water containing 100 and 200 ppm DMAV for 10 weeks. Transforming growth factor-β (TGF-β) immunoexpression in rat bladder epithelium and urine level of IL-1β also were determined. We found that DMAV dramatically increased the mRNA levels of NF-κB p50 and IKKα in the bladder epithelium of rats compared to the control group. Immunohistochemical examinations showed that DMAV increased immunoreactivities of IKKα, IKKβ, and phospho-NF-κB p50 in the cytoplasm and phospho-NF-κB p50 and p65 in nucleus of rat urothelial cells. In addition, DMAV treated rats exhibited significantly increased inflammatory factor TGF-β immunoreactivity in bladder epithelium and IL-1β secretion in urine. These data suggest that DMAV could activate NF-κB signal pathway and increase TGF-β and IL-1β expressions in bladder epithelial cells of rats.http://dx.doi.org/10.1155/2015/790652 |
| spellingShingle | Siqi Cao Shengnan Liu Fei Wang Jieyu Liu Mengdan Li Chen Wang Shuhua Xi DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats Mediators of Inflammation |
| title | DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats |
| title_full | DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats |
| title_fullStr | DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats |
| title_full_unstemmed | DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats |
| title_short | DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats |
| title_sort | dmav in drinking water activated nf κb signal pathway and increased tgf β and il 1β expressions in bladder epithelial cells of rats |
| url | http://dx.doi.org/10.1155/2015/790652 |
| work_keys_str_mv | AT siqicao dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT shengnanliu dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT feiwang dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT jieyuliu dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT mengdanli dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT chenwang dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats AT shuhuaxi dmavindrinkingwateractivatednfkbsignalpathwayandincreasedtgfbandil1bexpressionsinbladderepithelialcellsofrats |