Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration
Cancer-related deaths primarily occur due to metastasis, a process involving the migration and invasion of cancer cells. In most solid tumors, metastasis occurs through collective cell migration (CCM), guided by “cellular leaders”. These leader cells generate forces through actomyosin-mediated protr...
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2025-01-01
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| author | Anastasia Alexandrova Elizaveta Kontareva Margarita Pustovalova Sergey Leonov Yulia Merkher |
| author_facet | Anastasia Alexandrova Elizaveta Kontareva Margarita Pustovalova Sergey Leonov Yulia Merkher |
| author_sort | Anastasia Alexandrova |
| collection | DOAJ |
| description | Cancer-related deaths primarily occur due to metastasis, a process involving the migration and invasion of cancer cells. In most solid tumors, metastasis occurs through collective cell migration (CCM), guided by “cellular leaders”. These leader cells generate forces through actomyosin-mediated protrusion and contractility. The cytoskeletal mechanisms employed by metastatic cells during the migration process closely resemble the use of the actin cytoskeleton in endocytosis. In our previous work, we revealed that tumor cells exhibiting high metastatic potential (MP) are more adept at encapsulating 100–200 nm nanoparticles than those with lower MP. The objective of this study was to investigate whether nanoparticle encapsulation could effectively differentiate leader tumor cells during their CCM. To achieve our objectives, we employed a two-dimensional CCM model grounded in the wound-healing (“scratch”) assay, utilizing two breast cancer cell lines, MCF7 and MDA-MB-231, which display low and high migratory potential, respectively. We conducted calibration experiments to identify the “optimal time” at which cells exhibit peak speed during wound closure. Furthermore, we carried out experiments to assess nanoparticle uptake, calculating the colocalization coefficient, and employed phalloidin staining to analyze the anisotropy and orientation of actin filaments. The highest activity for low-MP cells was achieved at 2.6 h during the calibration experiments, whereas high-MP cells were maximally active at 3.9 h, resulting in 8% and 11% reductions in wound area, respectively. We observed a significant difference in encapsulation efficiency between leader and peripheral cells for both high-MP (<i>p</i> < 0.013) and low-MP (<i>p</i> < 0.02) cells. Moreover, leader cells demonstrated a considerably higher anisotropy coefficient (<i>p</i> < 0.029), indicating a more organized, directional structure of actin filaments compared to peripheral cells. Thus, nanoparticle encapsulation offers a groundbreaking approach to identifying the most aggressive and invasive leader cells during the CCM process in breast cancer. Detecting these cells is crucial for developing targeted therapies that can effectively curb metastasis and improve patient outcomes. |
| format | Article |
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| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-a9cf00d4bfdf43239ef3b92c8db325dc2025-01-24T13:38:54ZengMDPI AGLife2075-17292025-01-0115112710.3390/life15010127Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During MigrationAnastasia Alexandrova0Elizaveta Kontareva1Margarita Pustovalova2Sergey Leonov3Yulia Merkher4The Laboratory of Personalized Chemo-Radiation Therapy, Institute of Future Biophysics, Moscow 141700, RussiaThe Laboratory of Personalized Chemo-Radiation Therapy, Institute of Future Biophysics, Moscow 141700, RussiaThe Laboratory of Personalized Chemo-Radiation Therapy, Institute of Future Biophysics, Moscow 141700, RussiaThe Laboratory of Personalized Chemo-Radiation Therapy, Institute of Future Biophysics, Moscow 141700, RussiaThe Laboratory of Personalized Chemo-Radiation Therapy, Institute of Future Biophysics, Moscow 141700, RussiaCancer-related deaths primarily occur due to metastasis, a process involving the migration and invasion of cancer cells. In most solid tumors, metastasis occurs through collective cell migration (CCM), guided by “cellular leaders”. These leader cells generate forces through actomyosin-mediated protrusion and contractility. The cytoskeletal mechanisms employed by metastatic cells during the migration process closely resemble the use of the actin cytoskeleton in endocytosis. In our previous work, we revealed that tumor cells exhibiting high metastatic potential (MP) are more adept at encapsulating 100–200 nm nanoparticles than those with lower MP. The objective of this study was to investigate whether nanoparticle encapsulation could effectively differentiate leader tumor cells during their CCM. To achieve our objectives, we employed a two-dimensional CCM model grounded in the wound-healing (“scratch”) assay, utilizing two breast cancer cell lines, MCF7 and MDA-MB-231, which display low and high migratory potential, respectively. We conducted calibration experiments to identify the “optimal time” at which cells exhibit peak speed during wound closure. Furthermore, we carried out experiments to assess nanoparticle uptake, calculating the colocalization coefficient, and employed phalloidin staining to analyze the anisotropy and orientation of actin filaments. The highest activity for low-MP cells was achieved at 2.6 h during the calibration experiments, whereas high-MP cells were maximally active at 3.9 h, resulting in 8% and 11% reductions in wound area, respectively. We observed a significant difference in encapsulation efficiency between leader and peripheral cells for both high-MP (<i>p</i> < 0.013) and low-MP (<i>p</i> < 0.02) cells. Moreover, leader cells demonstrated a considerably higher anisotropy coefficient (<i>p</i> < 0.029), indicating a more organized, directional structure of actin filaments compared to peripheral cells. Thus, nanoparticle encapsulation offers a groundbreaking approach to identifying the most aggressive and invasive leader cells during the CCM process in breast cancer. Detecting these cells is crucial for developing targeted therapies that can effectively curb metastasis and improve patient outcomes.https://www.mdpi.com/2075-1729/15/1/127metastasiscytoskeletal mechanismsmetastatic potentialendocytosisnanoparticle encapsulationactin filaments |
| spellingShingle | Anastasia Alexandrova Elizaveta Kontareva Margarita Pustovalova Sergey Leonov Yulia Merkher Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration Life metastasis cytoskeletal mechanisms metastatic potential endocytosis nanoparticle encapsulation actin filaments |
| title | Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration |
| title_full | Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration |
| title_fullStr | Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration |
| title_full_unstemmed | Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration |
| title_short | Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration |
| title_sort | navigating the collective nanoparticle assisted identification of leader cancer cells during migration |
| topic | metastasis cytoskeletal mechanisms metastatic potential endocytosis nanoparticle encapsulation actin filaments |
| url | https://www.mdpi.com/2075-1729/15/1/127 |
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