Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition
Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2011/201254 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557046402121728 |
|---|---|
| author | Eduardo J. Gudiña José A. Teixeira Lígia R. Rodrigues |
| author_facet | Eduardo J. Gudiña José A. Teixeira Lígia R. Rodrigues |
| author_sort | Eduardo J. Gudiña |
| collection | DOAJ |
| description | Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1 and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1 Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use of L. paracasei ssp. paracasei A20 as a promising biosurfactant-producer. |
| format | Article |
| id | doaj-art-a98e04793bbf4656995ee4a15a0041f4 |
| institution | Kabale University |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-a98e04793bbf4656995ee4a15a0041f42025-02-03T05:43:45ZengWileyApplied and Environmental Soil Science1687-76671687-76752011-01-01201110.1155/2011/201254201254Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium CompositionEduardo J. Gudiña0José A. Teixeira1Lígia R. Rodrigues2Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalInstitute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalInstitute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalBiosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved with Lactobacillus paracasei ssp. paracasei A20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1 and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1 Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use of L. paracasei ssp. paracasei A20 as a promising biosurfactant-producer.http://dx.doi.org/10.1155/2011/201254 |
| spellingShingle | Eduardo J. Gudiña José A. Teixeira Lígia R. Rodrigues Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition Applied and Environmental Soil Science |
| title | Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition |
| title_full | Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition |
| title_fullStr | Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition |
| title_full_unstemmed | Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition |
| title_short | Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition |
| title_sort | biosurfactant producing lactobacilli screening production profiles and effect of medium composition |
| url | http://dx.doi.org/10.1155/2011/201254 |
| work_keys_str_mv | AT eduardojgudina biosurfactantproducinglactobacilliscreeningproductionprofilesandeffectofmediumcomposition AT joseateixeira biosurfactantproducinglactobacilliscreeningproductionprofilesandeffectofmediumcomposition AT ligiarrodrigues biosurfactantproducinglactobacilliscreeningproductionprofilesandeffectofmediumcomposition |