Screening of six medicinal plant species for antileishmanial activity
This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonoc...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2021-09-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2021-0023 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832570519902224384 |
|---|---|
| author | Ferreira Fabiana Borges Padilha Cabral Márcia Regina Pereira Sarragiotto Maria Helena Fernandez Carla Maria Mariano Gazim Zilda Cristiani Junior Ranulfo Piau Demarchi Izabel Galhardo Silveira Thaís Gomes Verzignassi Lonardoni Maria Valdrinez Campana |
| author_facet | Ferreira Fabiana Borges Padilha Cabral Márcia Regina Pereira Sarragiotto Maria Helena Fernandez Carla Maria Mariano Gazim Zilda Cristiani Junior Ranulfo Piau Demarchi Izabel Galhardo Silveira Thaís Gomes Verzignassi Lonardoni Maria Valdrinez Campana |
| author_sort | Ferreira Fabiana Borges Padilha |
| collection | DOAJ |
| description | This study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %. |
| format | Article |
| id | doaj-art-a98318030d1a4e3c92777fc4ab736e8f |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-a98318030d1a4e3c92777fc4ab736e8f2025-02-02T15:22:06ZengSciendoActa Pharmaceutica1846-95582021-09-0171339941410.2478/acph-2021-0023acph-2021-0023Screening of six medicinal plant species for antileishmanial activityFerreira Fabiana Borges Padilha0Cabral Márcia Regina Pereira1Sarragiotto Maria Helena2Fernandez Carla Maria Mariano3Gazim Zilda Cristiani4Junior Ranulfo Piau5Demarchi Izabel Galhardo6Silveira Thaís Gomes Verzignassi7Lonardoni Maria Valdrinez Campana8Posgraduate Program in Health Sciences Maringá State University, , BrazilPosgraduate Program in Pharmaceutical Sciences, Maringá State University, Maringá – Paraná State, BrazilPostgraduate Program in Chemistry, Maringá State University, Maringá – Paraná, BrazilPosgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Umuarama – Paraná State, BrazilPosgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Umuarama – Paraná State, BrazilPosgraduate Program in Animal Science of the University of Paranaense, Universidade Paranaense UNIPAR, Umuarama – Paraná State, BrazilFederal University of Santa Catarina, Florianópolis – Santa Catarina State, BrazilPosgraduate Program in Health Sciences Maringá State University, , BrazilPosgraduate Program in Health Sciences Maringá State University, , BrazilThis study is aimed to investigate the in vitro anti-leishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 μg mL−1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 μg mL−1 for L. amazonensis and > 200 μg mL−1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 μg mL−1 and hemolytic activity from 0 to 46.1 %.https://doi.org/10.2478/acph-2021-0023piper corcovadensishymenaea stigonocarpaantileishmanialleishmania amazonensisleishmania braziliensiscytotoxicityhemolysis |
| spellingShingle | Ferreira Fabiana Borges Padilha Cabral Márcia Regina Pereira Sarragiotto Maria Helena Fernandez Carla Maria Mariano Gazim Zilda Cristiani Junior Ranulfo Piau Demarchi Izabel Galhardo Silveira Thaís Gomes Verzignassi Lonardoni Maria Valdrinez Campana Screening of six medicinal plant species for antileishmanial activity Acta Pharmaceutica piper corcovadensis hymenaea stigonocarpa antileishmanial leishmania amazonensis leishmania braziliensis cytotoxicity hemolysis |
| title | Screening of six medicinal plant species for antileishmanial activity |
| title_full | Screening of six medicinal plant species for antileishmanial activity |
| title_fullStr | Screening of six medicinal plant species for antileishmanial activity |
| title_full_unstemmed | Screening of six medicinal plant species for antileishmanial activity |
| title_short | Screening of six medicinal plant species for antileishmanial activity |
| title_sort | screening of six medicinal plant species for antileishmanial activity |
| topic | piper corcovadensis hymenaea stigonocarpa antileishmanial leishmania amazonensis leishmania braziliensis cytotoxicity hemolysis |
| url | https://doi.org/10.2478/acph-2021-0023 |
| work_keys_str_mv | AT ferreirafabianaborgespadilha screeningofsixmedicinalplantspeciesforantileishmanialactivity AT cabralmarciareginapereira screeningofsixmedicinalplantspeciesforantileishmanialactivity AT sarragiottomariahelena screeningofsixmedicinalplantspeciesforantileishmanialactivity AT fernandezcarlamariamariano screeningofsixmedicinalplantspeciesforantileishmanialactivity AT gazimzildacristiani screeningofsixmedicinalplantspeciesforantileishmanialactivity AT juniorranulfopiau screeningofsixmedicinalplantspeciesforantileishmanialactivity AT demarchiizabelgalhardo screeningofsixmedicinalplantspeciesforantileishmanialactivity AT silveirathaisgomesverzignassi screeningofsixmedicinalplantspeciesforantileishmanialactivity AT lonardonimariavaldrinezcampana screeningofsixmedicinalplantspeciesforantileishmanialactivity |