Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor
Biosustainability, toxicity, and the potential for disruption of aquatic ecosystems and human health have made azo dyes into a serious environmental issue in industrial wastewater. In this context, a group of bacteria isolated from dye-contaminated wastewater was used in a moving bed bioreactor with...
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Water and Wastewater Consulting Engineers Research Development
2024-08-01
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| Series: | آب و فاضلاب |
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| Online Access: | https://www.wwjournal.ir/article_211679_45b63975d9906c218f7debba3354175f.pdf |
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| author | Soroush Dayani Najafabadi Maryam Hasani Zonoozi Roya Aliaskarian |
| author_facet | Soroush Dayani Najafabadi Maryam Hasani Zonoozi Roya Aliaskarian |
| author_sort | Soroush Dayani Najafabadi |
| collection | DOAJ |
| description | Biosustainability, toxicity, and the potential for disruption of aquatic ecosystems and human health have made azo dyes into a serious environmental issue in industrial wastewater. In this context, a group of bacteria isolated from dye-contaminated wastewater was used in a moving bed bioreactor with polyurethane foam biocarriers for the biodegradation of Congo red azo dye. According to the EzBioCloud database (eztaxon), the dominant bacterium identified through DNA extraction, PCR, and 16S-rRNA sequencing showed the highest similarity to Lysinibacillus Capsici PB300(T). The efficiency of dye and chemical oxygen demand (COD) removal in the MBBR was examined in relation to process time, filling ratio, and dye concentration. Dye removal in this system was optimized, maintaining the pH at 7 ± 0.2, the temperature at 35 ± 1.0 °C, and COD at 980 mg/L. The highest removal efficiencies for dye and COD were 92% and 85.1%, respectively, after 72 hours at a dye concentration of 50 mg/L and a filling ratio of 40%. Kinetic modeling was employed to study biodegradation. The R² value for the first-order model was 0.9457, while for the second-order model it was 0.9791. The second-order kinetic model (Grau's equation) provided a more accurate prediction of dye degradation in the MBBR system. This study confirms the successful degradation of Congo red azo dye using immobilized bacteria on Polyurethane foam (PUF) biocarriers in the MBBR bioreactor. |
| format | Article |
| id | doaj-art-a97ad54dc4b9430e8e1caef07adee140 |
| institution | Kabale University |
| issn | 1024-5936 2383-0905 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Water and Wastewater Consulting Engineers Research Development |
| record_format | Article |
| series | آب و فاضلاب |
| spelling | doaj-art-a97ad54dc4b9430e8e1caef07adee1402025-02-01T07:44:57ZengWater and Wastewater Consulting Engineers Research Developmentآب و فاضلاب1024-59362383-09052024-08-01353456810.22093/wwj.2024.474675.3432211679Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed BioreactorSoroush Dayani Najafabadi0Maryam Hasani Zonoozi1Roya Aliaskarian2Master of Environmental Engineering, Dept. of Civil Engineering, Iran University of Science and Technology (IUST), Tehran, IranAssist. Prof., Water and Environmental Engineering Group, Dept. of Civil Engineering, Iran University of Science and Technology (IUST), Tehran, IranMaster of Microbiology, Dept. of Molecular Cell Biology and Microbiology, Isfahan University, Isfahan, IranBiosustainability, toxicity, and the potential for disruption of aquatic ecosystems and human health have made azo dyes into a serious environmental issue in industrial wastewater. In this context, a group of bacteria isolated from dye-contaminated wastewater was used in a moving bed bioreactor with polyurethane foam biocarriers for the biodegradation of Congo red azo dye. According to the EzBioCloud database (eztaxon), the dominant bacterium identified through DNA extraction, PCR, and 16S-rRNA sequencing showed the highest similarity to Lysinibacillus Capsici PB300(T). The efficiency of dye and chemical oxygen demand (COD) removal in the MBBR was examined in relation to process time, filling ratio, and dye concentration. Dye removal in this system was optimized, maintaining the pH at 7 ± 0.2, the temperature at 35 ± 1.0 °C, and COD at 980 mg/L. The highest removal efficiencies for dye and COD were 92% and 85.1%, respectively, after 72 hours at a dye concentration of 50 mg/L and a filling ratio of 40%. Kinetic modeling was employed to study biodegradation. The R² value for the first-order model was 0.9457, while for the second-order model it was 0.9791. The second-order kinetic model (Grau's equation) provided a more accurate prediction of dye degradation in the MBBR system. This study confirms the successful degradation of Congo red azo dye using immobilized bacteria on Polyurethane foam (PUF) biocarriers in the MBBR bioreactor.https://www.wwjournal.ir/article_211679_45b63975d9906c218f7debba3354175f.pdfcongo red azo dyepolyurethane foammoving bed bioreactorlysinibacillus capsici pb300(t)biodegradation |
| spellingShingle | Soroush Dayani Najafabadi Maryam Hasani Zonoozi Roya Aliaskarian Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor آب و فاضلاب congo red azo dye polyurethane foam moving bed bioreactor lysinibacillus capsici pb300(t) biodegradation |
| title | Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor |
| title_full | Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor |
| title_fullStr | Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor |
| title_full_unstemmed | Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor |
| title_short | Biodegradation of Congo Red Azo Dye Using Lysinibacillus Capsici Immobilized on Polyurethane foam Biocarrier in Moving Bed Bioreactor |
| title_sort | biodegradation of congo red azo dye using lysinibacillus capsici immobilized on polyurethane foam biocarrier in moving bed bioreactor |
| topic | congo red azo dye polyurethane foam moving bed bioreactor lysinibacillus capsici pb300(t) biodegradation |
| url | https://www.wwjournal.ir/article_211679_45b63975d9906c218f7debba3354175f.pdf |
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