Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4

Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4

ABSTRACT Bacterial plasmid encoding antibiotic resistance could be eradicated by various CRISPR systems, such as CRISPR-Cas9, Cas12f1, and Cas3. However, the efficacy of these gene editing tools against bacterial resistance has not been systematically assessed and compared. This study eliminates car...

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Bibliographic Details
Main Authors: Jun Huang, Kanghui Ding, Jiahui Chen, Jiao Fan, Luyao Huang, Shaofu Qiu, Ligui Wang, Xinying Du, Chao Wang, Haifeng Pan, Zhengquan Yuan, Hongbo Liu, Hongbin Song
Format: Article
Language:English
Published: American Society for Microbiology 2025-06-01
Series:Microbiology Spectrum
Subjects:
antimicrobial resistance
KPC-2
IMP-4
CRISPR-Cas9
CRISPR-Cas12f1
CRISPR-Cas3
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02572-24
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https://journals.asm.org/doi/10.1128/spectrum.02572-24

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