The prethalamus is established during gastrulation and influences diencephalic regionalization.
The vertebrate neural plate contains distinct domains of gene expression, prefiguring the future brain areas. In this study, we draw an extended expression map of the rostral neural plate that reveals discrete domains inside the presumptive posterior forebrain. We show, by fate mapping, that these w...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-04-01
|
| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050069&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849686654442274816 |
|---|---|
| author | Nicole Staudt Corinne Houart |
| author_facet | Nicole Staudt Corinne Houart |
| author_sort | Nicole Staudt |
| collection | DOAJ |
| description | The vertebrate neural plate contains distinct domains of gene expression, prefiguring the future brain areas. In this study, we draw an extended expression map of the rostral neural plate that reveals discrete domains inside the presumptive posterior forebrain. We show, by fate mapping, that these well-defined cell populations will develop into specific diencephalic regions. To address whether these early subterritories are already committed to restricted identities, we began to analyse the consequences of ablation and transplantation of these specific cell populations. We found that precursors of the prethalamus are already specified and irreplaceable at late gastrula stage, because ablation of these cells results in loss of prethalamic markers. Moreover, when transplanted into the ectopic environment of the presumptive hindbrain, these cells still pursue their prethalamic differentiation program. Finally, transplantation of these precursors, in the rostral-most neural epithelium, induces changes in cell identity in the surrounding host forebrain. This cell-non-autonomous property led us to propose that these committed prethalamic precursors may play an instructive role in the regionalization of the developing diencephalon. |
| format | Article |
| id | doaj-art-a8f3b0b5095d4d51ab54e45bb9fe2c98 |
| institution | DOAJ |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2007-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-a8f3b0b5095d4d51ab54e45bb9fe2c982025-08-20T03:22:37ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-04-0154e6910.1371/journal.pbio.0050069The prethalamus is established during gastrulation and influences diencephalic regionalization.Nicole StaudtCorinne HouartThe vertebrate neural plate contains distinct domains of gene expression, prefiguring the future brain areas. In this study, we draw an extended expression map of the rostral neural plate that reveals discrete domains inside the presumptive posterior forebrain. We show, by fate mapping, that these well-defined cell populations will develop into specific diencephalic regions. To address whether these early subterritories are already committed to restricted identities, we began to analyse the consequences of ablation and transplantation of these specific cell populations. We found that precursors of the prethalamus are already specified and irreplaceable at late gastrula stage, because ablation of these cells results in loss of prethalamic markers. Moreover, when transplanted into the ectopic environment of the presumptive hindbrain, these cells still pursue their prethalamic differentiation program. Finally, transplantation of these precursors, in the rostral-most neural epithelium, induces changes in cell identity in the surrounding host forebrain. This cell-non-autonomous property led us to propose that these committed prethalamic precursors may play an instructive role in the regionalization of the developing diencephalon.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050069&type=printable |
| spellingShingle | Nicole Staudt Corinne Houart The prethalamus is established during gastrulation and influences diencephalic regionalization. PLoS Biology |
| title | The prethalamus is established during gastrulation and influences diencephalic regionalization. |
| title_full | The prethalamus is established during gastrulation and influences diencephalic regionalization. |
| title_fullStr | The prethalamus is established during gastrulation and influences diencephalic regionalization. |
| title_full_unstemmed | The prethalamus is established during gastrulation and influences diencephalic regionalization. |
| title_short | The prethalamus is established during gastrulation and influences diencephalic regionalization. |
| title_sort | prethalamus is established during gastrulation and influences diencephalic regionalization |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050069&type=printable |
| work_keys_str_mv | AT nicolestaudt theprethalamusisestablishedduringgastrulationandinfluencesdiencephalicregionalization AT corinnehouart theprethalamusisestablishedduringgastrulationandinfluencesdiencephalicregionalization AT nicolestaudt prethalamusisestablishedduringgastrulationandinfluencesdiencephalicregionalization AT corinnehouart prethalamusisestablishedduringgastrulationandinfluencesdiencephalicregionalization |