Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model
This study was aimed to develop an ECM-derived biphasic scaffold and to investigate its regeneration potential loaded with BM-MSCs in repair of large, high-load-bearing osteochondral defects of the canine femoral head. The scaffolds were fabricated using cartilage and bone ECM as a cartilage and bon...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/127084 |
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| author | Yang Qiang Zhao Yanhong Peng Jiang Lu Shibi Guo Quanyi Ma Xinlong Xia Qun Xu Baoshan Zhao Bin Wang Aiyuan Zhang Li Xu Wengjing Zeng Chao |
| author_facet | Yang Qiang Zhao Yanhong Peng Jiang Lu Shibi Guo Quanyi Ma Xinlong Xia Qun Xu Baoshan Zhao Bin Wang Aiyuan Zhang Li Xu Wengjing Zeng Chao |
| author_sort | Yang Qiang |
| collection | DOAJ |
| description | This study was aimed to develop an ECM-derived biphasic scaffold and to investigate its regeneration potential loaded with BM-MSCs in repair of large, high-load-bearing osteochondral defects of the canine femoral head. The scaffolds were fabricated using cartilage and bone ECM as a cartilage and bone layer, respectively. Osteochondral constructs were fabricated using induced BM-MSCs and the scaffold. Osteochondral defects (11 mm diameter × 10 mm depth) were created on femoral heads of canine and treated with the constructs. The repaired tissue was evaluated for gross morphology, radiography, histological, biomechanics at 3 and 6 months after implantation. Radiography revealed that femoral heads slightly collapsed at 3 months and severely collapsed at 6 months. Histology revealed that some defects in femoral heads were repaired, but with fibrous tissue or fibrocartilage, and femoral heads with different degrees of collapse. The bone volume fraction was lower for subchondral bone than normal femoral bone at 3 and 6 months. Rigidity was lower in repaired subchondral bone than normal femoral bone at 6 months. The ECM-derived, biphasic scaffold combined with induced BM-MSCs did not successfully repair large, high-load-bearing osteochondral defects of the canine femoral head. However, the experience can help improve the technique of scaffold fabrication and vascularization. |
| format | Article |
| id | doaj-art-a8d6c182b9e04b3eacc4fbe12742c73c |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a8d6c182b9e04b3eacc4fbe12742c73c2025-02-03T05:59:14ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/127084127084Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine ModelYang Qiang0Zhao Yanhong1Peng Jiang2Lu Shibi3Guo Quanyi4Ma Xinlong5Xia Qun6Xu Baoshan7Zhao Bin8Wang Aiyuan9Zhang Li10Xu Wengjing11Zeng Chao12Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaStomatological Hospital of Tianjin Medical University, Tianjin 300070, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Spine Surgery, Tianjin Hospital, Tianjin 300211, ChinaDepartment of Spine Surgery, Tianjin Hospital, Tianjin 300211, ChinaDepartment of Spine Surgery, Tianjin Hospital, Tianjin 300211, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaInstitute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Spine Surgery, Tianjin Hospital, Tianjin 300211, ChinaThis study was aimed to develop an ECM-derived biphasic scaffold and to investigate its regeneration potential loaded with BM-MSCs in repair of large, high-load-bearing osteochondral defects of the canine femoral head. The scaffolds were fabricated using cartilage and bone ECM as a cartilage and bone layer, respectively. Osteochondral constructs were fabricated using induced BM-MSCs and the scaffold. Osteochondral defects (11 mm diameter × 10 mm depth) were created on femoral heads of canine and treated with the constructs. The repaired tissue was evaluated for gross morphology, radiography, histological, biomechanics at 3 and 6 months after implantation. Radiography revealed that femoral heads slightly collapsed at 3 months and severely collapsed at 6 months. Histology revealed that some defects in femoral heads were repaired, but with fibrous tissue or fibrocartilage, and femoral heads with different degrees of collapse. The bone volume fraction was lower for subchondral bone than normal femoral bone at 3 and 6 months. Rigidity was lower in repaired subchondral bone than normal femoral bone at 6 months. The ECM-derived, biphasic scaffold combined with induced BM-MSCs did not successfully repair large, high-load-bearing osteochondral defects of the canine femoral head. However, the experience can help improve the technique of scaffold fabrication and vascularization.http://dx.doi.org/10.1155/2014/127084 |
| spellingShingle | Yang Qiang Zhao Yanhong Peng Jiang Lu Shibi Guo Quanyi Ma Xinlong Xia Qun Xu Baoshan Zhao Bin Wang Aiyuan Zhang Li Xu Wengjing Zeng Chao Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model The Scientific World Journal |
| title | Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model |
| title_full | Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model |
| title_fullStr | Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model |
| title_full_unstemmed | Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model |
| title_short | Xenoimplantation of an Extracellular-Matrix-Derived, Biphasic, Cell-Scaffold Construct for Repairing a Large Femoral-Head High-Load-Bearing Osteochondral Defect in a Canine Model |
| title_sort | xenoimplantation of an extracellular matrix derived biphasic cell scaffold construct for repairing a large femoral head high load bearing osteochondral defect in a canine model |
| url | http://dx.doi.org/10.1155/2014/127084 |
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