Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat
Inflammatory processes have been implicated in the pathogenesis of traumatic brain damage. We analyzed the spatiotemporal expression pattern of the proinflammatory key molecules: interleukin-1β, interleukin-6, tumor necrosis factor-α, and inducible nitric oxide synthase in a rat closed head injury (...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/90123 |
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| _version_ | 1832558054339510272 |
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| author | Servan Rooker Sebastian Jander Jos Van Reempts Guido Stoll Philippe G. Jorens Marcel Borgers Jan Verlooy |
| author_facet | Servan Rooker Sebastian Jander Jos Van Reempts Guido Stoll Philippe G. Jorens Marcel Borgers Jan Verlooy |
| author_sort | Servan Rooker |
| collection | DOAJ |
| description | Inflammatory processes have been implicated in the pathogenesis of
traumatic brain damage. We analyzed the spatiotemporal expression
pattern of the proinflammatory key molecules: interleukin-1β,
interleukin-6, tumor necrosis factor-α, and inducible nitric
oxide synthase in a rat closed head injury (CHI) paradigm. 51 rats
were used for RT-PCR analysis after CHI, and 18 for
immunocytochemistry. We found an early upregulation of
IL-1β, IL-6, and TNF-α mRNA between 1 h and
7 h after injury; the expression of iNOS mRNA only revealed a
significant increase at 4 h. After 24 h, the expression
decreased towards baseline levels, and remained low until 7 d
after injury. Immunocytochemically, IL-1β induction was
localized to ramified microglia in areas surrounding the primary
impact place as well as deeper brain structures. Our study shows
rapid induction of inflammatory gene expression that exceeds by
far the primary impact site and might therefore contribute to
tissue damage at remote sites. |
| format | Article |
| id | doaj-art-a8884422addc48ea92106fb3f8ac8c84 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a8884422addc48ea92106fb3f8ac8c842025-02-03T01:33:27ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/9012390123Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the RatServan Rooker0Sebastian Jander1Jos Van Reempts2Guido Stoll3Philippe G. Jorens4Marcel Borgers5Jan Verlooy6Department of Neurosurgery, University Hospital Antwerp, Edegem 2650, BelgiumDepartment of Neurology, Heinrich-Heine University, Düsseldorf 40225, GermanyDepartment of Life Sciences, Janssen Research Foundation, Beerse 2340, BelgiumDepartment of Neurology, Heinrich-Heine University, Düsseldorf 40225, GermanyDepartment of Intensive Care Medicine, University Hospital Antwerp, Edegem 2650, BelgiumDepartment of Life Sciences, Janssen Research Foundation, Beerse 2340, BelgiumDepartment of Neurosurgery, University Hospital Antwerp, Edegem 2650, BelgiumInflammatory processes have been implicated in the pathogenesis of traumatic brain damage. We analyzed the spatiotemporal expression pattern of the proinflammatory key molecules: interleukin-1β, interleukin-6, tumor necrosis factor-α, and inducible nitric oxide synthase in a rat closed head injury (CHI) paradigm. 51 rats were used for RT-PCR analysis after CHI, and 18 for immunocytochemistry. We found an early upregulation of IL-1β, IL-6, and TNF-α mRNA between 1 h and 7 h after injury; the expression of iNOS mRNA only revealed a significant increase at 4 h. After 24 h, the expression decreased towards baseline levels, and remained low until 7 d after injury. Immunocytochemically, IL-1β induction was localized to ramified microglia in areas surrounding the primary impact place as well as deeper brain structures. Our study shows rapid induction of inflammatory gene expression that exceeds by far the primary impact site and might therefore contribute to tissue damage at remote sites.http://dx.doi.org/10.1155/MI/2006/90123 |
| spellingShingle | Servan Rooker Sebastian Jander Jos Van Reempts Guido Stoll Philippe G. Jorens Marcel Borgers Jan Verlooy Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat Mediators of Inflammation |
| title | Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat |
| title_full | Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat |
| title_fullStr | Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat |
| title_full_unstemmed | Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat |
| title_short | Spatiotemporal Pattern of Neuroinflammation After Impact-Acceleration Closed Head Injury in the Rat |
| title_sort | spatiotemporal pattern of neuroinflammation after impact acceleration closed head injury in the rat |
| url | http://dx.doi.org/10.1155/MI/2006/90123 |
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