Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes
Type 1 diabetes (T1D) is related to the autoimmune destruction of β-cells, leading to their almost complete absence in patients with longstanding T1D. However, endogenous insulin secretion persists in such patients as evidenced by the measurement of plasma C-peptide. Recently, a low level of insulin...
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MDPI AG
2025-01-01
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| author | Yuliya Krivova Alexandra Proshchina Dmitry Otlyga Anastasia Kharlamova Sergey Saveliev |
| author_facet | Yuliya Krivova Alexandra Proshchina Dmitry Otlyga Anastasia Kharlamova Sergey Saveliev |
| author_sort | Yuliya Krivova |
| collection | DOAJ |
| description | Type 1 diabetes (T1D) is related to the autoimmune destruction of β-cells, leading to their almost complete absence in patients with longstanding T1D. However, endogenous insulin secretion persists in such patients as evidenced by the measurement of plasma C-peptide. Recently, a low level of insulin has been found in non-β islet cells of patients with longstanding T1D, indicating that other islet cell types may contribute to persistent insulin secretion. The present study aimed to test the ability of various antibodies to detect insulin in insulin-deficient islets of T1D patients. Pancreatic autopsies from two children with recent-onset T1D, two adults with longstanding T1D, and three control subjects were examined using double immunofluorescent labeling with antibodies to insulin, glucagon and somatostatin. Immunoreactivity to insulin in glucagon+ cells of insulin-deficient islets was revealed using polyclonal antibodies and monoclonal antibodies simultaneously recognizing insulin and proinsulin. Along with this, immunoreactivity to insulin was observed in the majority of glucagon+ cells of insulin-containing islets of control subjects and children with recent-onset T1D. These results suggest that islet α-cells may contain insulin and/or other insulin-like proteins (proinsulin, C-peptide). Future studies are needed to evaluate the role of α-cells in insulin secretion and diabetes pathogenesis. |
| format | Article |
| id | doaj-art-a8030fac025e48fa86b7a7c27ef23604 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj-art-a8030fac025e48fa86b7a7c27ef236042025-01-24T13:38:54ZengMDPI AGLife2075-17292025-01-0115112510.3390/life15010125Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 DiabetesYuliya Krivova0Alexandra Proshchina1Dmitry Otlyga2Anastasia Kharlamova3Sergey Saveliev4Laboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Tsurupi Street, 3, 117418 Moscow, RussiaLaboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Tsurupi Street, 3, 117418 Moscow, RussiaLaboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Tsurupi Street, 3, 117418 Moscow, RussiaLaboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Tsurupi Street, 3, 117418 Moscow, RussiaLaboratory of Nervous System Development, Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Tsurupi Street, 3, 117418 Moscow, RussiaType 1 diabetes (T1D) is related to the autoimmune destruction of β-cells, leading to their almost complete absence in patients with longstanding T1D. However, endogenous insulin secretion persists in such patients as evidenced by the measurement of plasma C-peptide. Recently, a low level of insulin has been found in non-β islet cells of patients with longstanding T1D, indicating that other islet cell types may contribute to persistent insulin secretion. The present study aimed to test the ability of various antibodies to detect insulin in insulin-deficient islets of T1D patients. Pancreatic autopsies from two children with recent-onset T1D, two adults with longstanding T1D, and three control subjects were examined using double immunofluorescent labeling with antibodies to insulin, glucagon and somatostatin. Immunoreactivity to insulin in glucagon+ cells of insulin-deficient islets was revealed using polyclonal antibodies and monoclonal antibodies simultaneously recognizing insulin and proinsulin. Along with this, immunoreactivity to insulin was observed in the majority of glucagon+ cells of insulin-containing islets of control subjects and children with recent-onset T1D. These results suggest that islet α-cells may contain insulin and/or other insulin-like proteins (proinsulin, C-peptide). Future studies are needed to evaluate the role of α-cells in insulin secretion and diabetes pathogenesis.https://www.mdpi.com/2075-1729/15/1/125human pancreastype 1 diabetesinsulinglucagonpolyhormonal cells |
| spellingShingle | Yuliya Krivova Alexandra Proshchina Dmitry Otlyga Anastasia Kharlamova Sergey Saveliev Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes Life human pancreas type 1 diabetes insulin glucagon polyhormonal cells |
| title | Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes |
| title_full | Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes |
| title_fullStr | Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes |
| title_full_unstemmed | Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes |
| title_short | Detection of Insulin in Insulin-Deficient Islets of Patients with Type 1 Diabetes |
| title_sort | detection of insulin in insulin deficient islets of patients with type 1 diabetes |
| topic | human pancreas type 1 diabetes insulin glucagon polyhormonal cells |
| url | https://www.mdpi.com/2075-1729/15/1/125 |
| work_keys_str_mv | AT yuliyakrivova detectionofinsulinininsulindeficientisletsofpatientswithtype1diabetes AT alexandraproshchina detectionofinsulinininsulindeficientisletsofpatientswithtype1diabetes AT dmitryotlyga detectionofinsulinininsulindeficientisletsofpatientswithtype1diabetes AT anastasiakharlamova detectionofinsulinininsulindeficientisletsofpatientswithtype1diabetes AT sergeysaveliev detectionofinsulinininsulindeficientisletsofpatientswithtype1diabetes |