Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency

Abstract Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, w...

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Bibliographic Details
Main Authors: Zhuran Zhao, Xi Chen, Shan Wang, Min Fu, Huan Shen, Jiayu Li, Jun Xu, Jiong Qin, Cheng Shi
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Cell & Bioscience
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Online Access:https://doi.org/10.1186/s13578-025-01410-0
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