Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate
Abstract Pyomelanogenic P. aeruginosa, frequently isolated from patients with urinary tract infections and cystic fibrosis, possesses the ability to withstand oxidative stress, contributing to virulence and resulting in persistent infections. Whole genome sequence analysis of U804, a pyomelanogenic,...
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Nature Portfolio
2025-01-01
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| author | Jayalekshmi Haripriyan Christy Rose Binu Nitasha D Menon Muralidharan Vanuopadath Malavika B. Hari N Namitha Kesiya Binoy Anil Kumar Bipin G. Nair Victor Nizet Geetha B. Kumar |
| author_facet | Jayalekshmi Haripriyan Christy Rose Binu Nitasha D Menon Muralidharan Vanuopadath Malavika B. Hari N Namitha Kesiya Binoy Anil Kumar Bipin G. Nair Victor Nizet Geetha B. Kumar |
| author_sort | Jayalekshmi Haripriyan |
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| description | Abstract Pyomelanogenic P. aeruginosa, frequently isolated from patients with urinary tract infections and cystic fibrosis, possesses the ability to withstand oxidative stress, contributing to virulence and resulting in persistent infections. Whole genome sequence analysis of U804, a pyomelanogenic, multidrug-resistant, clinical isolate, demonstrates the mechanism underlying pyomelanin overproduction. Seven essential oils (EOs) were screened for pyomelanin inhibition. Garlic, cinnamon and thyme EOs were selected for further studies based on their significant anti-virulent properties, like inhibition of pyomelanin production and biofilm formation. Additionally, downregulation of the expression of virulence genes regulated by quorum sensing (QS) and a decrease in levels of the QS signaling molecule, C12-HSL, were also observed. The EO treatment inhibited the survival of U804 in human blood and increased survival of C. elegans, a whole animal model of pathogenesis. EO treatment also resulted in a significant reduction of efflux pump activity, indicative of their effect on antibiotic sensitization. Garlic oil enhanced the permeability of the bacterial membrane, resulting in decreased survival, when combined with sub-MIC concentrations of colistin. This study demonstrates that thyme, cinnamon and garlic EOs can attenuate pyomelanogenic P. aeruginosa virulence traits. Additionally, garlic potentiates drug sensitivity, suggesting its promising therapeutic use in combating pyomelanogenic MDR infections. |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Scientific Reports |
| spelling | doaj-art-a7c9b3f6780a414db3a649a979ee0c6c2025-02-02T12:22:49ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-86515-9Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolateJayalekshmi Haripriyan0Christy Rose Binu1Nitasha D Menon2Muralidharan Vanuopadath3Malavika B. Hari4N Namitha5Kesiya Binoy6Anil Kumar7Bipin G. Nair8Victor Nizet9Geetha B. Kumar10School of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamDepartment of Microbiology, Amrita Institute of Medical Sciences, Amrita Vishwa VidyapeethamSchool of Biotechnology, Amrita Vishwa VidyapeethamDepartment of Pharmacology, University of California, San DiegoSchool of Biotechnology, Amrita Vishwa VidyapeethamAbstract Pyomelanogenic P. aeruginosa, frequently isolated from patients with urinary tract infections and cystic fibrosis, possesses the ability to withstand oxidative stress, contributing to virulence and resulting in persistent infections. Whole genome sequence analysis of U804, a pyomelanogenic, multidrug-resistant, clinical isolate, demonstrates the mechanism underlying pyomelanin overproduction. Seven essential oils (EOs) were screened for pyomelanin inhibition. Garlic, cinnamon and thyme EOs were selected for further studies based on their significant anti-virulent properties, like inhibition of pyomelanin production and biofilm formation. Additionally, downregulation of the expression of virulence genes regulated by quorum sensing (QS) and a decrease in levels of the QS signaling molecule, C12-HSL, were also observed. The EO treatment inhibited the survival of U804 in human blood and increased survival of C. elegans, a whole animal model of pathogenesis. EO treatment also resulted in a significant reduction of efflux pump activity, indicative of their effect on antibiotic sensitization. Garlic oil enhanced the permeability of the bacterial membrane, resulting in decreased survival, when combined with sub-MIC concentrations of colistin. This study demonstrates that thyme, cinnamon and garlic EOs can attenuate pyomelanogenic P. aeruginosa virulence traits. Additionally, garlic potentiates drug sensitivity, suggesting its promising therapeutic use in combating pyomelanogenic MDR infections.https://doi.org/10.1038/s41598-025-86515-9P. aeruginosaPyomelaninEssential oilsVirulence |
| spellingShingle | Jayalekshmi Haripriyan Christy Rose Binu Nitasha D Menon Muralidharan Vanuopadath Malavika B. Hari N Namitha Kesiya Binoy Anil Kumar Bipin G. Nair Victor Nizet Geetha B. Kumar Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate Scientific Reports P. aeruginosa Pyomelanin Essential oils Virulence |
| title | Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate |
| title_full | Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate |
| title_fullStr | Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate |
| title_full_unstemmed | Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate |
| title_short | Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate |
| title_sort | essential oils modulate virulence phenotypes in a multidrug resistant pyomelanogenic pseudomonas aeruginosa clinical isolate |
| topic | P. aeruginosa Pyomelanin Essential oils Virulence |
| url | https://doi.org/10.1038/s41598-025-86515-9 |
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