Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants
Metallic molybdenum (Mo) has been increasingly recognized as a potential biodegradable metal for biomedical implants. However, the macrophage-mediated inflammatory responses to Mo-based implants remain underexplored. This study examined the in vitro inflammatory reactions of macrophages to the degra...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000778 |
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| author | Danyang Liu Jiahao Chen Jiannan Zhou Jingtao Dai Haotian Qin Guojiang Wan Junyu Qian Ping Li Jiang Li |
| author_facet | Danyang Liu Jiahao Chen Jiannan Zhou Jingtao Dai Haotian Qin Guojiang Wan Junyu Qian Ping Li Jiang Li |
| author_sort | Danyang Liu |
| collection | DOAJ |
| description | Metallic molybdenum (Mo) has been increasingly recognized as a potential biodegradable metal for biomedical implants. However, the macrophage-mediated inflammatory responses to Mo-based implants remain underexplored. This study examined the in vitro inflammatory reactions of macrophages to the degradation products of biodegradable Mo implants. The short-term and long-term biodegradation behavior and the subsequent impact on cytotoxicity, metabolism, and macrophage polarization were assessed. Both Mo and its degradation products were shown to be non-toxic within macrophage tolerance limits. Nevertheless, morphological changes and pro-inflammatory polarization were observed in cells around Mo-based specimen. Notably, matrix metalloproteinase 9 (Mmp9) was identified as a key gene influencing macrophage polarization in proximity to Mo. Additionally, pre-treating the Mo specimens in culture medium for 24 h significantly mitigated its stimulatory effects on cells. These results demonstrated the significance of optimizing Mo pre-treatment methods to prevent localized inflammation associated with its degradation. Specifically, pre-treatment of Mo can effectively mitigate the adverse impacts of its early degradation on macrophages and the surrounding immune environment. Our research into these early degradation phases introduces new avenues for studying molybdenum's immunomodulatory properties, potentially through precise control of its release and the targeted expression of pivotal genes. |
| format | Article |
| id | doaj-art-a7a108f515d4441b8d5cc05f821ba0a7 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-a7a108f515d4441b8d5cc05f821ba0a72025-02-05T04:32:35ZengElsevierMaterials Today Bio2590-00642025-04-0131101519Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implantsDanyang Liu0Jiahao Chen1Jiannan Zhou2Jingtao Dai3Haotian Qin4Guojiang Wan5Junyu Qian6Ping Li7Jiang Li8School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, 510182, PR ChinaDepartment of Prosthodontics, Geriatric Dentistry and Craniomandibular Disorders, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Aßmannshauser Str. 4-6, 14197, Berlin, GermanySchool and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, 510182, PR ChinaDepartment of Orthodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, 510515, PR ChinaDepartment of Bone and Joint Surgery, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, 518036, PR ChinaInstitute of Biomedical Engineering, College of Medicine, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, 610031, PR ChinaDepartment of Bone and Joint Surgery, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, 518036, PR China; Corresponding author.School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, 510182, PR China; Corresponding author. School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China.School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, 510182, PR China; Corresponding author. School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510182, PR China.Metallic molybdenum (Mo) has been increasingly recognized as a potential biodegradable metal for biomedical implants. However, the macrophage-mediated inflammatory responses to Mo-based implants remain underexplored. This study examined the in vitro inflammatory reactions of macrophages to the degradation products of biodegradable Mo implants. The short-term and long-term biodegradation behavior and the subsequent impact on cytotoxicity, metabolism, and macrophage polarization were assessed. Both Mo and its degradation products were shown to be non-toxic within macrophage tolerance limits. Nevertheless, morphological changes and pro-inflammatory polarization were observed in cells around Mo-based specimen. Notably, matrix metalloproteinase 9 (Mmp9) was identified as a key gene influencing macrophage polarization in proximity to Mo. Additionally, pre-treating the Mo specimens in culture medium for 24 h significantly mitigated its stimulatory effects on cells. These results demonstrated the significance of optimizing Mo pre-treatment methods to prevent localized inflammation associated with its degradation. Specifically, pre-treatment of Mo can effectively mitigate the adverse impacts of its early degradation on macrophages and the surrounding immune environment. Our research into these early degradation phases introduces new avenues for studying molybdenum's immunomodulatory properties, potentially through precise control of its release and the targeted expression of pivotal genes.http://www.sciencedirect.com/science/article/pii/S2590006425000778MolybdenumBiodegradable metalMacrophage polarizationBiodegradabilityImmunoregulationInflammatory reaction |
| spellingShingle | Danyang Liu Jiahao Chen Jiannan Zhou Jingtao Dai Haotian Qin Guojiang Wan Junyu Qian Ping Li Jiang Li Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants Materials Today Bio Molybdenum Biodegradable metal Macrophage polarization Biodegradability Immunoregulation Inflammatory reaction |
| title | Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants |
| title_full | Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants |
| title_fullStr | Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants |
| title_full_unstemmed | Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants |
| title_short | Macrophage-related inflammatory responses to degradation products of biodegradable molybdenum implants |
| title_sort | macrophage related inflammatory responses to degradation products of biodegradable molybdenum implants |
| topic | Molybdenum Biodegradable metal Macrophage polarization Biodegradability Immunoregulation Inflammatory reaction |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000778 |
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