Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization
Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA (G-308A, C-857T, and C-1031T), IL10 (C-571A and A-1117G), and TGFB (C-509-T) and these sensitizations. The...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/276345 |
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| author | Blanca Cárdaba David Calzada Selene Baos Miriam Aguerri Joaquín Quiralte Carlos Lahoz |
| author_facet | Blanca Cárdaba David Calzada Selene Baos Miriam Aguerri Joaquín Quiralte Carlos Lahoz |
| author_sort | Blanca Cárdaba |
| collection | DOAJ |
| description | Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA (G-308A, C-857T, and C-1031T), IL10 (C-571A and A-1117G), and TGFB (C-509-T) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygous TGFB C-509T genotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations, CC genotype of IL10 C-571A could be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26, P=0.008). In contrast, heterozygous CA genotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygous TNFA G-308A genotype was associated with Ole e 10 sensitization (OR: 2.5, P=0.04). In conclusion, these results suggest a role of TGF-β1 in olive-pollen sensitization and TNF-α and IL-10 genotypes in the asthma induced by specific olive-pollen allergens. |
| format | Article |
| id | doaj-art-a783f392317d471bb8671f6e4766a8a8 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a783f392317d471bb8671f6e4766a8a82025-02-03T05:57:47ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/276345276345Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen SensitizationBlanca Cárdaba0David Calzada1Selene Baos2Miriam Aguerri3Joaquín Quiralte4Carlos Lahoz5Immunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, SpainImmunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, SpainImmunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, SpainImmunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, SpainAllergy Department, Hospital Universitario Vírgen del Rocío, Avenida Manuel Siurot, s/n, 41013 Seville, SpainImmunology Department, IIS-Fundación Jiménez Díaz, UAM, Avenida Reyes Católicos No. 2, 28040 Madrid, SpainSensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims of TNFA (G-308A, C-857T, and C-1031T), IL10 (C-571A and A-1117G), and TGFB (C-509-T) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygous TGFB C-509T genotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations, CC genotype of IL10 C-571A could be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26, P=0.008). In contrast, heterozygous CA genotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygous TNFA G-308A genotype was associated with Ole e 10 sensitization (OR: 2.5, P=0.04). In conclusion, these results suggest a role of TGF-β1 in olive-pollen sensitization and TNF-α and IL-10 genotypes in the asthma induced by specific olive-pollen allergens.http://dx.doi.org/10.1155/2014/276345 |
| spellingShingle | Blanca Cárdaba David Calzada Selene Baos Miriam Aguerri Joaquín Quiralte Carlos Lahoz Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization Journal of Immunology Research |
| title | Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization |
| title_full | Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization |
| title_fullStr | Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization |
| title_full_unstemmed | Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization |
| title_short | Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization |
| title_sort | polymorphisms of tumor necrosis factor α transforming growth factor β and interleukin 10 in asthma associated with olive pollen sensitization |
| url | http://dx.doi.org/10.1155/2014/276345 |
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