Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification

Strand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we...

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Bibliographic Details
Main Authors: Yinhua Zhang, Ashley N Luck, Nathan A Tanner
Format: Article
Language:English
Published: Taylor & Francis Group 2024-06-01
Series:BioTechniques
Subjects:
Online Access:https://www.tandfonline.com/doi/10.2144/btn-2024-0012
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