Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Strand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2024-06-01
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Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.tandfonline.com/doi/10.2144/btn-2024-0012 |
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