A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii
Background.The iron/siderophore uptake system (IUS) involved in the Acinetobacter baumannii pathogenicity. However, IUS’s role in antibiotic resistance and the production of β-lactamase enzymes of A. baumannii are unclear. This study aimed to investigate the relationship between the production of β-...
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| Format: | Article |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/5565537 |
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| author | Mahyar Porbaran Hamed Tahmasebi MohammadReza Arabestani |
| author_facet | Mahyar Porbaran Hamed Tahmasebi MohammadReza Arabestani |
| author_sort | Mahyar Porbaran |
| collection | DOAJ |
| description | Background.The iron/siderophore uptake system (IUS) involved in the Acinetobacter baumannii pathogenicity. However, IUS’s role in antibiotic resistance and the production of β-lactamase enzymes of A. baumannii are unclear. This study aimed to investigate the relationship between the production of β-lactamase enzymes and IUS regulatory genes in clinical isolates of A. baumannii. Methods. A. baumannii isolates were collected from clinical isolates using biochemical tests. The antibiotic resistance patterns and β-lactamase-producing strains were identified using the disk diffusion method (DDM). Also, IUS genes were detected by the polymerase chain reaction (PCR) method. Results. Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. Gentamicin-resistant strains (43%) had the highest frequency, and aztreonam-resistant strains (12.5%) had the lowest frequency. Also, the distribution of AmpC and MBL producing isolates were 27.7% and 35%, respectively. Moreover, the frequencies of basD, bauA, pld, paaE, entA, feoB, hemO, and tonB genes were as follows: 12.5%, 15.2%, 11.1%, 15.2%, 19.4%, 16.6%, 23.6%, and 6.9%. Further, a strong correlation was observed between the abundance of β-lactamase-producing strains and IUS genes. Conclusions. Based on our knowledge from this study, the association between β-lactamase production and IUS genes in A. baumannii plays an essential role in the emergence of drug-resistant strains. |
| format | Article |
| id | doaj-art-a7437ea6b6d4468687568cc595aeec3f |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-a7437ea6b6d4468687568cc595aeec3f2025-02-03T01:05:20ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/55655375565537A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumanniiMahyar Porbaran0Hamed Tahmasebi1MohammadReza Arabestani2Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Hamedan Branch, Hamedan, IranSchool of Medicine, Shahroud University of Medical Sciences, Shahroud, IranMicrobiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IranBackground.The iron/siderophore uptake system (IUS) involved in the Acinetobacter baumannii pathogenicity. However, IUS’s role in antibiotic resistance and the production of β-lactamase enzymes of A. baumannii are unclear. This study aimed to investigate the relationship between the production of β-lactamase enzymes and IUS regulatory genes in clinical isolates of A. baumannii. Methods. A. baumannii isolates were collected from clinical isolates using biochemical tests. The antibiotic resistance patterns and β-lactamase-producing strains were identified using the disk diffusion method (DDM). Also, IUS genes were detected by the polymerase chain reaction (PCR) method. Results. Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. Gentamicin-resistant strains (43%) had the highest frequency, and aztreonam-resistant strains (12.5%) had the lowest frequency. Also, the distribution of AmpC and MBL producing isolates were 27.7% and 35%, respectively. Moreover, the frequencies of basD, bauA, pld, paaE, entA, feoB, hemO, and tonB genes were as follows: 12.5%, 15.2%, 11.1%, 15.2%, 19.4%, 16.6%, 23.6%, and 6.9%. Further, a strong correlation was observed between the abundance of β-lactamase-producing strains and IUS genes. Conclusions. Based on our knowledge from this study, the association between β-lactamase production and IUS genes in A. baumannii plays an essential role in the emergence of drug-resistant strains.http://dx.doi.org/10.1155/2021/5565537 |
| spellingShingle | Mahyar Porbaran Hamed Tahmasebi MohammadReza Arabestani A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii International Journal of Microbiology |
| title | A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii |
| title_full | A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii |
| title_fullStr | A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii |
| title_full_unstemmed | A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii |
| title_short | A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii |
| title_sort | comprehensive study of the relationship between the production of β lactamase enzymes and iron siderophore uptake regulatory genes in clinical isolates of acinetobacter baumannii |
| url | http://dx.doi.org/10.1155/2021/5565537 |
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