Precision targeting of genetic variations in mixed bacterial cultures using CRISPR-Cas12a-programmed λ phages

The CRISPR-Cas system, an adaptive immune mechanism in prokaryotes against bacteriophages, has been developed into a versatile tool for recognizing and cleaving target nucleic acid sequences. In this study, we developed a model system by integrating CRISPR-Cas12a into the genome of temperate bacteri...

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Bibliographic Details
Main Authors: Chan Kyeong Lee, Ho Joung Lee, Song Hee Jeong, Sang Jun Lee
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1575339/full
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