Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3
GABA-producing probiotics present promising opportunities for developing functional foods. Carbon sources have been identified as a critical influence on GABA production. Therefore, this study investigated the holistic metabolic responses and GABA biosynthesis to various carbon sources of Lactiplant...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025002592 |
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| author | Nachon Raethong Pitakthai Chamtim Roypim Thananusak Kanyawee Whanmek Chalat Santivarangkna |
| author_facet | Nachon Raethong Pitakthai Chamtim Roypim Thananusak Kanyawee Whanmek Chalat Santivarangkna |
| author_sort | Nachon Raethong |
| collection | DOAJ |
| description | GABA-producing probiotics present promising opportunities for developing functional foods. Carbon sources have been identified as a critical influence on GABA production. Therefore, this study investigated the holistic metabolic responses and GABA biosynthesis to various carbon sources of Lactiplantibacillus pentosus 9D3, a proficient GABA producer, using a genome-wide transcriptomic approach. The analysis revealed 414 genes with differential expression responses to altering carbon sources, i.e., glucose, sucrose, and lactose, notably sugar phosphotransferase systems (PTS) (11 genes), indicating carbon source-mediated transcriptional change patterns in L. pentosus 9D3. The integration of transcriptome data with a genome-scale metabolic network (GSMN) revealed that L. pentosus 9D3 displays adaptability by synthesizing GABA as an alternative acid-tolerant mechanism when lactose is used as a carbon source rather than depending on the fatty acid synthesis and the arginine catabolic pathway. The findings of this study offer valuable insights into optimal carbon source utilization and gene expression co-regulation, thereby enhancing the GABA-producing capability of a probiotic and broadening its potential applications in the functional food industry. |
| format | Article |
| id | doaj-art-a6f83ebec36d4572885331e09616d36f |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-a6f83ebec36d4572885331e09616d36f2025-02-02T05:28:27ZengElsevierHeliyon2405-84402025-01-01112e41879Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3Nachon Raethong0Pitakthai Chamtim1Roypim Thananusak2Kanyawee Whanmek3Chalat Santivarangkna4Institute of Nutrition, Mahidol University, Nakhon Pathom, 73170, Thailand; Corresponding author.Academic Service Division, National Laboratory Animal Center, Mahidol University, Nakhon Pathom, 73170, ThailandOmics Center for Agriculture, Bioresource, Food and Health Kasetsart University (OmiKU), Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Duckweed Holobiont Resource & Research Center (DHbRC), Faculty of Science, Kasetsart University, Bangkok, 10900, ThailandInstitute of Nutrition, Mahidol University, Nakhon Pathom, 73170, ThailandInstitute of Nutrition, Mahidol University, Nakhon Pathom, 73170, ThailandGABA-producing probiotics present promising opportunities for developing functional foods. Carbon sources have been identified as a critical influence on GABA production. Therefore, this study investigated the holistic metabolic responses and GABA biosynthesis to various carbon sources of Lactiplantibacillus pentosus 9D3, a proficient GABA producer, using a genome-wide transcriptomic approach. The analysis revealed 414 genes with differential expression responses to altering carbon sources, i.e., glucose, sucrose, and lactose, notably sugar phosphotransferase systems (PTS) (11 genes), indicating carbon source-mediated transcriptional change patterns in L. pentosus 9D3. The integration of transcriptome data with a genome-scale metabolic network (GSMN) revealed that L. pentosus 9D3 displays adaptability by synthesizing GABA as an alternative acid-tolerant mechanism when lactose is used as a carbon source rather than depending on the fatty acid synthesis and the arginine catabolic pathway. The findings of this study offer valuable insights into optimal carbon source utilization and gene expression co-regulation, thereby enhancing the GABA-producing capability of a probiotic and broadening its potential applications in the functional food industry.http://www.sciencedirect.com/science/article/pii/S2405844025002592 |
| spellingShingle | Nachon Raethong Pitakthai Chamtim Roypim Thananusak Kanyawee Whanmek Chalat Santivarangkna Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 Heliyon |
| title | Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 |
| title_full | Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 |
| title_fullStr | Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 |
| title_full_unstemmed | Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 |
| title_short | Genome-wide transcriptomics revealed carbon source-mediated gamma-aminobutyric acid (GABA) production in a probiotic, Lactiplantibacillus pentosus 9D3 |
| title_sort | genome wide transcriptomics revealed carbon source mediated gamma aminobutyric acid gaba production in a probiotic lactiplantibacillus pentosus 9d3 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025002592 |
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