Studies of the Kinetic Characteristics of HSA Adsorption by Different Types of Activated Carbon Fibres
Adsorption of human serum albumin (HSA) from aqueous solution on to activated carbon fibres (ACF) with different degrees of activation in the range 0–70% has been studied at 293 ± 1K. The various parameters for the porous structure were calculated by different methods on the basis of the low-tempera...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2000-06-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263617001493558 |
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| Summary: | Adsorption of human serum albumin (HSA) from aqueous solution on to activated carbon fibres (ACF) with different degrees of activation in the range 0–70% has been studied at 293 ± 1K. The various parameters for the porous structure were calculated by different methods on the basis of the low-temperature adsorption isotherms of nitrogen measured at 77 ± 1 K. It was shown that an increase in the degree of ACF activation led to the development of a porous structure which exhibited increasing values of the micropore and mesopore volumes. Relationships between the structure, the physicochemical properties of the surface and the adsorption characteristics of ACF were established. The kinetic curves for HSA adsorption from aqueous solution at an initial concentration of 0.5 mg/ml were characterized by negative adsorption ranges. This phenomenon may be explained, on the one hand, by the energetic heterogeneity of the protein–surface interaction and, on the other hand, by the possibility of alterations in the conformations of the protein molecules at the interface. The latter suggestion was confirmed by the hyperchromous shift observed in the UV spectrum of the HSA solution after protein–surface contact had been established. |
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| ISSN: | 0263-6174 2048-4038 |