Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro
Homeostatic disruptor agents, and endocrine disruptor compounds (EDC) specifically, can originate from agricultural and industrial chemicals. If they modify the adaptation of living organisms as direct (e.g., by altering hormone regulation, membrane functions) and/or indirect (e.g., cell transformat...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2019/1546131 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548908311511040 |
|---|---|
| author | Krisztián Sepp Zsolt Molnár Anna M. László Tünde Alapi László Tóth Andrea Serester Zsuzsanna Valkusz Márta Gálfi Marianna Radács |
| author_facet | Krisztián Sepp Zsolt Molnár Anna M. László Tünde Alapi László Tóth Andrea Serester Zsuzsanna Valkusz Márta Gálfi Marianna Radács |
| author_sort | Krisztián Sepp |
| collection | DOAJ |
| description | Homeostatic disruptor agents, and endocrine disruptor compounds (EDC) specifically, can originate from agricultural and industrial chemicals. If they modify the adaptation of living organisms as direct (e.g., by altering hormone regulation, membrane functions) and/or indirect (e.g., cell transformation mechanisms) factors, they are classified as EDC. We aimed to examine the potential endocrine-disrupting effects of phenylurea herbicides (phenuron, monuron, and diuron) on the oxytocin (OT) and arginine-vasopressin (AVP) release of neurohypophysis cell cultures (NH). In our experiments, monoamine-activated receptor functions of neurohypophyseal cells were used as a model. In vitro NH were prepared by enzymatic (trypsin, collagenase) and mechanical dissociation. In the experimental protocol, the basal levels of OT and AVP were determined as controls. Later, monoamine (epinephrine, norepinephrine, serotonin, histamine, and dopamine) activation (10−6 M, 30 min) and the effects of phenylurea (10−6 M, 60 min) alone and in combination (monoamines 10−6 M, 30 min + phenylureas 10−6 M, 60 min) with monoamine were studied. OT and AVP hormone contents in the supernatant media were measured by radioimmunoassay. The monoamine-activated receptor functions of neurohypophyseal cells were modified by the applied doses of phenuron, monuron, and diuron. It is concluded that the applied phenylurea herbicides are endocrine disruptor agents, at least in vitro for neurohypophysis function. |
| format | Article |
| id | doaj-art-a6dd57cfce0c4810b3573926c53d1f32 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-a6dd57cfce0c4810b3573926c53d1f322025-02-03T06:12:47ZengWileyInternational Journal of Endocrinology1687-83371687-83452019-01-01201910.1155/2019/15461311546131Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In VitroKrisztián Sepp0Zsolt Molnár1Anna M. László2Tünde Alapi3László Tóth4Andrea Serester5Zsuzsanna Valkusz6Márta Gálfi7Marianna Radács8First Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged, HungaryInstitute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, HungaryDepartment of Biometrics and Agricultural Informatics, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Inorganic and Analytical Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, HungaryInstitute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, HungaryInstitute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, HungaryFirst Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged, HungaryInstitute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, HungaryInstitute of Applied Science, Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, University of Szeged, Szeged, HungaryHomeostatic disruptor agents, and endocrine disruptor compounds (EDC) specifically, can originate from agricultural and industrial chemicals. If they modify the adaptation of living organisms as direct (e.g., by altering hormone regulation, membrane functions) and/or indirect (e.g., cell transformation mechanisms) factors, they are classified as EDC. We aimed to examine the potential endocrine-disrupting effects of phenylurea herbicides (phenuron, monuron, and diuron) on the oxytocin (OT) and arginine-vasopressin (AVP) release of neurohypophysis cell cultures (NH). In our experiments, monoamine-activated receptor functions of neurohypophyseal cells were used as a model. In vitro NH were prepared by enzymatic (trypsin, collagenase) and mechanical dissociation. In the experimental protocol, the basal levels of OT and AVP were determined as controls. Later, monoamine (epinephrine, norepinephrine, serotonin, histamine, and dopamine) activation (10−6 M, 30 min) and the effects of phenylurea (10−6 M, 60 min) alone and in combination (monoamines 10−6 M, 30 min + phenylureas 10−6 M, 60 min) with monoamine were studied. OT and AVP hormone contents in the supernatant media were measured by radioimmunoassay. The monoamine-activated receptor functions of neurohypophyseal cells were modified by the applied doses of phenuron, monuron, and diuron. It is concluded that the applied phenylurea herbicides are endocrine disruptor agents, at least in vitro for neurohypophysis function.http://dx.doi.org/10.1155/2019/1546131 |
| spellingShingle | Krisztián Sepp Zsolt Molnár Anna M. László Tünde Alapi László Tóth Andrea Serester Zsuzsanna Valkusz Márta Gálfi Marianna Radács Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro International Journal of Endocrinology |
| title | Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro |
| title_full | Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro |
| title_fullStr | Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro |
| title_full_unstemmed | Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro |
| title_short | Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro |
| title_sort | study of the potential endocrine disrupting effects of phenylurea compounds on neurohypophysis cells in vitro |
| url | http://dx.doi.org/10.1155/2019/1546131 |
| work_keys_str_mv | AT krisztiansepp studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT zsoltmolnar studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT annamlaszlo studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT tundealapi studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT laszlototh studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT andreaserester studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT zsuzsannavalkusz studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT martagalfi studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro AT mariannaradacs studyofthepotentialendocrinedisruptingeffectsofphenylureacompoundsonneurohypophysiscellsinvitro |