Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation
Obesity, the fifth leading cause of death globally and linked to chronic low-grade inflammation and development of numerous severe pathologies, is a major public health problem. Fermented foods, probiotics, and postbiotics emerge as promising avenues for combating obesity and inflammation. The aim o...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266592712500005X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591786954981376 |
|---|---|
| author | A. Chervet R. Nehme C. Defois-Fraysse C. Decombat C. Blavignac C. Auxenfans B. Evrard S. Michel E. Filaire J.-Y. Berthon A. Dreux-Zigha L. Delort F. Caldefie-Chézet |
| author_facet | A. Chervet R. Nehme C. Defois-Fraysse C. Decombat C. Blavignac C. Auxenfans B. Evrard S. Michel E. Filaire J.-Y. Berthon A. Dreux-Zigha L. Delort F. Caldefie-Chézet |
| author_sort | A. Chervet |
| collection | DOAJ |
| description | Obesity, the fifth leading cause of death globally and linked to chronic low-grade inflammation and development of numerous severe pathologies, is a major public health problem. Fermented foods, probiotics, and postbiotics emerge as promising avenues for combating obesity and inflammation. The aim of our study was to develop and characterize phyto-postbiotics corresponding to prebiotic compounds fermented by gut bacteria, which could act on obesity and related-inflammation. Chicory extract fermented by Akkermansia muciniphila (C-Akm) was selected as the most antioxidant of 20 fermented extracts. The identification of metabolites derived from C-Akm extract has enabled us to detect mostly amino acids, acids, and some polyphenols (daidzein and genistein). The anti-inflammatory and anti-obesity activities of C-Akm extract were studied by testing the extract (50 μg/mL) on the polarization of THP-1 into macrophages, the secretion of pro-inflammatory cytokines in LPS-stimulated PBMCs, and the secretion of leptin and adiponectin in adipospheroids derived from human adipose stem cells. Finally, the extract was examined in 3D co-culture model mimicking inflamed obese adipose tissue. We found that C-Akm extract decreased ROS generation, TNF-α and Il-6 gene expression in polarized macrophages, INFγ and IL-17A secretion in LPS-stimulated PBMCs stimulated with LPS. It also decreased leptin expression while increasing adiponectin and HSL expression levels in both adipocytes and co-cultures. In addition, C-Akm extract stimulated adiponectin secretion in the co-culture model. Finally, our in vitro investigations demonstrated the potential benefits of C-Akm extract in the prevention and treatment of obesity-related inflammation. |
| format | Article |
| id | doaj-art-a67a5c72abcb4aa78732a6f4975d72e5 |
| institution | Kabale University |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-a67a5c72abcb4aa78732a6f4975d72e52025-01-22T05:44:02ZengElsevierCurrent Research in Food Science2665-92712025-01-0110100974Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammationA. Chervet0R. Nehme1C. Defois-Fraysse2C. Decombat3C. Blavignac4C. Auxenfans5B. Evrard6S. Michel7E. Filaire8J.-Y. Berthon9A. Dreux-Zigha10L. Delort11F. Caldefie-Chézet12Université Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceGreencell, Biopôle Clermont-Limagne, 63360, Saint-Beauzire, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceUniversité Clermont-Auvergne, Centre d’Imagerie Cellulaire Santé (CCIS), Clermont-Ferrand, FranceBanque de Tissus et de Cellules, Hôpital Edouard-Herriot, 69000, Lyon, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceGreentech, Biopôle Clermont-Limagne, 63360, Saint-Beauzire, FranceGreencell, Biopôle Clermont-Limagne, 63360, Saint-Beauzire, FranceUniversité Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, France; Corresponding author.Université Clermont-Auvergne, INRAE, UNH, Unité de Nutrition Humaine, CRNH-Auvergne, 63000, Clermont-Ferrand, FranceObesity, the fifth leading cause of death globally and linked to chronic low-grade inflammation and development of numerous severe pathologies, is a major public health problem. Fermented foods, probiotics, and postbiotics emerge as promising avenues for combating obesity and inflammation. The aim of our study was to develop and characterize phyto-postbiotics corresponding to prebiotic compounds fermented by gut bacteria, which could act on obesity and related-inflammation. Chicory extract fermented by Akkermansia muciniphila (C-Akm) was selected as the most antioxidant of 20 fermented extracts. The identification of metabolites derived from C-Akm extract has enabled us to detect mostly amino acids, acids, and some polyphenols (daidzein and genistein). The anti-inflammatory and anti-obesity activities of C-Akm extract were studied by testing the extract (50 μg/mL) on the polarization of THP-1 into macrophages, the secretion of pro-inflammatory cytokines in LPS-stimulated PBMCs, and the secretion of leptin and adiponectin in adipospheroids derived from human adipose stem cells. Finally, the extract was examined in 3D co-culture model mimicking inflamed obese adipose tissue. We found that C-Akm extract decreased ROS generation, TNF-α and Il-6 gene expression in polarized macrophages, INFγ and IL-17A secretion in LPS-stimulated PBMCs stimulated with LPS. It also decreased leptin expression while increasing adiponectin and HSL expression levels in both adipocytes and co-cultures. In addition, C-Akm extract stimulated adiponectin secretion in the co-culture model. Finally, our in vitro investigations demonstrated the potential benefits of C-Akm extract in the prevention and treatment of obesity-related inflammation.http://www.sciencedirect.com/science/article/pii/S266592712500005XAkkermansia muciniphilaFermentationPrebioticsPostbioticsObesityInflammation |
| spellingShingle | A. Chervet R. Nehme C. Defois-Fraysse C. Decombat C. Blavignac C. Auxenfans B. Evrard S. Michel E. Filaire J.-Y. Berthon A. Dreux-Zigha L. Delort F. Caldefie-Chézet Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation Current Research in Food Science Akkermansia muciniphila Fermentation Prebiotics Postbiotics Obesity Inflammation |
| title | Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation |
| title_full | Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation |
| title_fullStr | Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation |
| title_full_unstemmed | Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation |
| title_short | Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation |
| title_sort | development and characterization of a chicory extract fermented by akkermansia muciniphila an in vitro study on its potential to modulate obesity related inflammation |
| topic | Akkermansia muciniphila Fermentation Prebiotics Postbiotics Obesity Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S266592712500005X |
| work_keys_str_mv | AT achervet developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT rnehme developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT cdefoisfraysse developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT cdecombat developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT cblavignac developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT cauxenfans developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT bevrard developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT smichel developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT efilaire developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT jyberthon developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT adreuxzigha developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT ldelort developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation AT fcaldefiechezet developmentandcharacterizationofachicoryextractfermentedbyakkermansiamuciniphilaaninvitrostudyonitspotentialtomodulateobesityrelatedinflammation |