Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides)
The enzymatic hydrolysis method was considered highly effective in hydrolyzing indigestible feathers into digestible proteins, however the practice of feather meal remained limited in aquafeed. Here, we evaluated the effects of hydrolyzed feather meal by keratinase (KFM), which breakdown keratin of...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000225 |
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| author | Yi-Huan Xu Li-Han Zhang Min Liu Mei-Yan Li Xue-Dan Fang Hui Xia Ran Guo Fazhan Hanafiah Waiho Khor Guang Chen Xin Hao |
| author_facet | Yi-Huan Xu Li-Han Zhang Min Liu Mei-Yan Li Xue-Dan Fang Hui Xia Ran Guo Fazhan Hanafiah Waiho Khor Guang Chen Xin Hao |
| author_sort | Yi-Huan Xu |
| collection | DOAJ |
| description | The enzymatic hydrolysis method was considered highly effective in hydrolyzing indigestible feathers into digestible proteins, however the practice of feather meal remained limited in aquafeed. Here, we evaluated the effects of hydrolyzed feather meal by keratinase (KFM), which breakdown keratin of feather to improve the protein utilization. Six diets were formulated. They respectively replaced fishmeal (FM) at 0 % (CON), 20 % (D1), 40 % (D2), 60 % (D3), 80 % (D4) and 100 % (D5) levels with KFM, and fed to 360 uniform-sized juvenile largemouth bass (initial weight, 14.84 ± 0.01 g) for 8 wk. According to analysis on weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR), we found that the largemouth bass had the best growth performance when dietary KFM replaced 25.19 %-26.25 % of FM, and the highest feed utilization when replacing 9.38 % of FM. Besides, the expression of fatty acid synthetase (fas) gene presented a linearly increased difference with the increasing KFM concentrations (P = 0.001), whereas the expression of adipose triglyceride lipase (atgl) gene was linearly decreased (P = 0.000), resulting in hepatic lipid accumulation in largemouth bass. Furthermore, there was a significant enhancement of antioxidant capacity along with oxidative stress in liver of largemouth bass with the increasing KFM concentration (P < 0.05). We concluded that no more than 55 % of FM replaced by KFM could benefit the metabolism of largemouth bass via the IGF/PI3K/AKT/TOR pathway. Moreover, when all dietary FM was replaced by KFM, the predominant intestinal flora was significantly inverted, resulting in histological abnormalities and low feed utilization of largemouth bass. Collectively, we suggested that approximate 25 % of FM replaced by KFM could benefit the growth and reduce fishmeal reliance in largemouth bass. |
| format | Article |
| id | doaj-art-a61b7cbf67fe4e2f85f48e6b0bb07e31 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-a61b7cbf67fe4e2f85f48e6b0bb07e312025-02-06T05:12:23ZengElsevierAquaculture Reports2352-51342025-03-0140102636Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides)Yi-Huan Xu0Li-Han Zhang1Min Liu2Mei-Yan Li3Xue-Dan Fang4Hui Xia5Ran Guo6Fazhan Hanafiah7Waiho Khor8Guang Chen9Xin Hao10Ocean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Hebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Hebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei 066000, China; Corresponding authors at: Ocean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China.Ocean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Tangshan Fisheries Technology Extension Station, Tangshan, Hebei 063016, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Hebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Hebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei 066000, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China; Hebei Key Laboratory of Nutritional Regulation and Disease Control for Aquaculture, Qinhuangdao, Hebei 066000, China; Corresponding authors at: Ocean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, China.Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, MalaysiaHigher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, MalaysiaQinhuangdao Yier Biotechnology Company Limited, Qinhuangdao, Hebei 066003, ChinaOcean College, Hebei Agricultural University, Qinhuangdao, Hebei 066000, ChinaThe enzymatic hydrolysis method was considered highly effective in hydrolyzing indigestible feathers into digestible proteins, however the practice of feather meal remained limited in aquafeed. Here, we evaluated the effects of hydrolyzed feather meal by keratinase (KFM), which breakdown keratin of feather to improve the protein utilization. Six diets were formulated. They respectively replaced fishmeal (FM) at 0 % (CON), 20 % (D1), 40 % (D2), 60 % (D3), 80 % (D4) and 100 % (D5) levels with KFM, and fed to 360 uniform-sized juvenile largemouth bass (initial weight, 14.84 ± 0.01 g) for 8 wk. According to analysis on weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR), we found that the largemouth bass had the best growth performance when dietary KFM replaced 25.19 %-26.25 % of FM, and the highest feed utilization when replacing 9.38 % of FM. Besides, the expression of fatty acid synthetase (fas) gene presented a linearly increased difference with the increasing KFM concentrations (P = 0.001), whereas the expression of adipose triglyceride lipase (atgl) gene was linearly decreased (P = 0.000), resulting in hepatic lipid accumulation in largemouth bass. Furthermore, there was a significant enhancement of antioxidant capacity along with oxidative stress in liver of largemouth bass with the increasing KFM concentration (P < 0.05). We concluded that no more than 55 % of FM replaced by KFM could benefit the metabolism of largemouth bass via the IGF/PI3K/AKT/TOR pathway. Moreover, when all dietary FM was replaced by KFM, the predominant intestinal flora was significantly inverted, resulting in histological abnormalities and low feed utilization of largemouth bass. Collectively, we suggested that approximate 25 % of FM replaced by KFM could benefit the growth and reduce fishmeal reliance in largemouth bass.http://www.sciencedirect.com/science/article/pii/S2352513425000225Keratinase-treated feather mealFeather mealLipid metabolismIntestinal microbiotaIGF signalingMicropterus salmoides |
| spellingShingle | Yi-Huan Xu Li-Han Zhang Min Liu Mei-Yan Li Xue-Dan Fang Hui Xia Ran Guo Fazhan Hanafiah Waiho Khor Guang Chen Xin Hao Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) Aquaculture Reports Keratinase-treated feather meal Feather meal Lipid metabolism Intestinal microbiota IGF signaling Micropterus salmoides |
| title | Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) |
| title_full | Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) |
| title_fullStr | Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) |
| title_full_unstemmed | Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) |
| title_short | Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) |
| title_sort | evaluation of the effects of keratinase treated feather meal as a replacement for fishmeal on growth performance liver function and intestinal health in juvenile largemouth bass micropterus salmoides |
| topic | Keratinase-treated feather meal Feather meal Lipid metabolism Intestinal microbiota IGF signaling Micropterus salmoides |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000225 |
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