Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products

<b>Background:</b> Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. <b>Objectives:</b> However, given the concerns of health-conscious consumers and the potential toxicity of synthetic subst...

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Main Authors: Marica Egidio, Loriana Casalino, Filomena De Biasio, Marika Di Paolo, Ricardo Gómez-García, Manuela Pintado, Alma Sardo, Raffaele Marrone
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Antibiotics
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Online Access:https://www.mdpi.com/2079-6382/13/10/932
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author Marica Egidio
Loriana Casalino
Filomena De Biasio
Marika Di Paolo
Ricardo Gómez-García
Manuela Pintado
Alma Sardo
Raffaele Marrone
author_facet Marica Egidio
Loriana Casalino
Filomena De Biasio
Marika Di Paolo
Ricardo Gómez-García
Manuela Pintado
Alma Sardo
Raffaele Marrone
author_sort Marica Egidio
collection DOAJ
description <b>Background:</b> Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. <b>Objectives:</b> However, given the concerns of health-conscious consumers and the potential toxicity of synthetic substances, this study aimed to explore the use of fennel waste extracts as natural preservatives. <b>Methods:</b> This study characterized the bioactive compounds (phenolic content), the antioxidant activity (ABTS<sup>+</sup> and DPPH assay), and the antimicrobial properties (against <i>Salmonella enterica</i> serotype Enteritidis, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Bacillus cereusi,</i> and <i>Pseudomonas aeruginosa</i>) of different fennel waste extracts (LF, liquid fraction; SF, solid fraction and PF, pellet fraction). Additionally, the potential use of the best fennel extract was evaluated for its impact on beef burger shelf life (up to 18 days at 4 ± 1 °C) in terms of microbiological profile, pH, and activity water (a<sub>w</sub>). <b>Results:</b> The PF extract, which was rich in flavones, hydroxybenzoic, and hydroxycinnamic acids, demonstrated the highest antioxidant and antimicrobial activities. Microbiological analyses on beef burgers with PF identified this extract as a potential antimicrobial substance. The a<sub>w</sub> and pH values did not appear to be affected. <b>Conclusions:</b> In conclusion, fennel extracts could be proposed as natural compounds exploitable in beef burgers to preserve their quality and extend their shelf-life.
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spelling doaj-art-a56cc31d9e74400a946aabe4e2d859012025-08-20T02:10:57ZengMDPI AGAntibiotics2079-63822024-10-01131093210.3390/antibiotics13100932Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat ProductsMarica Egidio0Loriana Casalino1Filomena De Biasio2Marika Di Paolo3Ricardo Gómez-García4Manuela Pintado5Alma Sardo6Raffaele Marrone7Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, ItalyDepartment of Economic and Legal Sciences, Universitas Mercatorum, 00186 Rome, ItalyEVRA S.r.l. Società Benefit, 85044 Lauria, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, ItalyCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, Italy<b>Background:</b> Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. <b>Objectives:</b> However, given the concerns of health-conscious consumers and the potential toxicity of synthetic substances, this study aimed to explore the use of fennel waste extracts as natural preservatives. <b>Methods:</b> This study characterized the bioactive compounds (phenolic content), the antioxidant activity (ABTS<sup>+</sup> and DPPH assay), and the antimicrobial properties (against <i>Salmonella enterica</i> serotype Enteritidis, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Bacillus cereusi,</i> and <i>Pseudomonas aeruginosa</i>) of different fennel waste extracts (LF, liquid fraction; SF, solid fraction and PF, pellet fraction). Additionally, the potential use of the best fennel extract was evaluated for its impact on beef burger shelf life (up to 18 days at 4 ± 1 °C) in terms of microbiological profile, pH, and activity water (a<sub>w</sub>). <b>Results:</b> The PF extract, which was rich in flavones, hydroxybenzoic, and hydroxycinnamic acids, demonstrated the highest antioxidant and antimicrobial activities. Microbiological analyses on beef burgers with PF identified this extract as a potential antimicrobial substance. The a<sub>w</sub> and pH values did not appear to be affected. <b>Conclusions:</b> In conclusion, fennel extracts could be proposed as natural compounds exploitable in beef burgers to preserve their quality and extend their shelf-life.https://www.mdpi.com/2079-6382/13/10/932minced meat productsadditivesnatural compoundsfennel extracts
spellingShingle Marica Egidio
Loriana Casalino
Filomena De Biasio
Marika Di Paolo
Ricardo Gómez-García
Manuela Pintado
Alma Sardo
Raffaele Marrone
Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
Antibiotics
minced meat products
additives
natural compounds
fennel extracts
title Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
title_full Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
title_fullStr Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
title_full_unstemmed Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
title_short Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products
title_sort antimicrobial properties of fennel by product extracts and their potential applications in meat products
topic minced meat products
additives
natural compounds
fennel extracts
url https://www.mdpi.com/2079-6382/13/10/932
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AT lorianacasalino antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT filomenadebiasio antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT marikadipaolo antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT ricardogomezgarcia antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT manuelapintado antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT almasardo antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts
AT raffaelemarrone antimicrobialpropertiesoffennelbyproductextractsandtheirpotentialapplicationsinmeatproducts