Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products

<b>Background:</b> Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. <b>Objectives:</b> However, given the concerns of health-conscious consumers and the potential toxicity of synthetic subst...

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Main Authors: Marica Egidio, Loriana Casalino, Filomena De Biasio, Marika Di Paolo, Ricardo Gómez-García, Manuela Pintado, Alma Sardo, Raffaele Marrone
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Antibiotics
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Online Access:https://www.mdpi.com/2079-6382/13/10/932
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Summary:<b>Background:</b> Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. <b>Objectives:</b> However, given the concerns of health-conscious consumers and the potential toxicity of synthetic substances, this study aimed to explore the use of fennel waste extracts as natural preservatives. <b>Methods:</b> This study characterized the bioactive compounds (phenolic content), the antioxidant activity (ABTS<sup>+</sup> and DPPH assay), and the antimicrobial properties (against <i>Salmonella enterica</i> serotype Enteritidis, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Bacillus cereusi,</i> and <i>Pseudomonas aeruginosa</i>) of different fennel waste extracts (LF, liquid fraction; SF, solid fraction and PF, pellet fraction). Additionally, the potential use of the best fennel extract was evaluated for its impact on beef burger shelf life (up to 18 days at 4 ± 1 °C) in terms of microbiological profile, pH, and activity water (a<sub>w</sub>). <b>Results:</b> The PF extract, which was rich in flavones, hydroxybenzoic, and hydroxycinnamic acids, demonstrated the highest antioxidant and antimicrobial activities. Microbiological analyses on beef burgers with PF identified this extract as a potential antimicrobial substance. The a<sub>w</sub> and pH values did not appear to be affected. <b>Conclusions:</b> In conclusion, fennel extracts could be proposed as natural compounds exploitable in beef burgers to preserve their quality and extend their shelf-life.
ISSN:2079-6382