Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients
Studies on the gastric microbiota associated with gastric precancerous lesions remain limited. This study aimed to profile the gastric mucosal microbiota in patients with <i>Helicobacter pylori</i>-negative precancerous lesions. Gastric mucosal samples were obtained from 67 <i>H. p...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/81 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587940369268736 |
|---|---|
| author | Han-Na Kim Min-Jeong Kim Jonathan P. Jacobs Hyo-Joon Yang |
| author_facet | Han-Na Kim Min-Jeong Kim Jonathan P. Jacobs Hyo-Joon Yang |
| author_sort | Han-Na Kim |
| collection | DOAJ |
| description | Studies on the gastric microbiota associated with gastric precancerous lesions remain limited. This study aimed to profile the gastric mucosal microbiota in patients with <i>Helicobacter pylori</i>-negative precancerous lesions. Gastric mucosal samples were obtained from 67 <i>H. pylori</i>-negative patients, including those with chronic gastritis (CG), intestinal metaplasia (IM), and dysplasia. The V3–V4 region of the 16S rRNA gene was sequenced and analyzed. No significant difference was observed in the alpha or beta diversity of the gastric microbiota among the groups. However, a taxonomic analysis revealed a significant enrichment of <i>Lautropia mirabilis</i> and the depletion of <i>Limosilactobacillus reuteri</i>, <i>Solobacxterium moorei</i>, <i>Haemophilus haemolyticus</i>, and <i>Duncaniella dubosii</i> in the IM and dysplasia groups compared to those in the CG group. <i>Prevotella jejuni</i> and the genus <i>Parvimonas</i> were enriched in the IM group. A predictive functional analysis revealed enrichment of the ornithine degradation pathway in the IM and dysplasia groups, suggesting its role in persistent gastric mucosal inflammation associated with gastric precancerous lesions. The gastric microbiota associated with <i>H. pylori</i>-negative gastric precancerous lesions showed an increased abundance of oral microbes linked to gastric cancer and a reduction in anti-inflammatory bacteria. These alterations might contribute to chronic gastric mucosal inflammation, promoting carcinogenesis in the absence of <i>H. pylori</i> infection. |
| format | Article |
| id | doaj-art-a4a632e4743440e9bcaa2bee0c1e4985 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-a4a632e4743440e9bcaa2bee0c1e49852025-01-24T13:42:34ZengMDPI AGMicroorganisms2076-26072025-01-011318110.3390/microorganisms13010081Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative PatientsHan-Na Kim0Min-Jeong Kim1Jonathan P. Jacobs2Hyo-Joon Yang3Department of Clinical Research Design and Evaluation, Samsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University, Seoul 06355, Republic of KoreaSamsung Advanced Institute for Health Sciences & Technology, Sungkyunkwan University, Seoul 06355, Republic of KoreaVatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USADivision of Gastroenterology, Department of Internal Medicine and Gastrointestinal Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 03181, Republic of KoreaStudies on the gastric microbiota associated with gastric precancerous lesions remain limited. This study aimed to profile the gastric mucosal microbiota in patients with <i>Helicobacter pylori</i>-negative precancerous lesions. Gastric mucosal samples were obtained from 67 <i>H. pylori</i>-negative patients, including those with chronic gastritis (CG), intestinal metaplasia (IM), and dysplasia. The V3–V4 region of the 16S rRNA gene was sequenced and analyzed. No significant difference was observed in the alpha or beta diversity of the gastric microbiota among the groups. However, a taxonomic analysis revealed a significant enrichment of <i>Lautropia mirabilis</i> and the depletion of <i>Limosilactobacillus reuteri</i>, <i>Solobacxterium moorei</i>, <i>Haemophilus haemolyticus</i>, and <i>Duncaniella dubosii</i> in the IM and dysplasia groups compared to those in the CG group. <i>Prevotella jejuni</i> and the genus <i>Parvimonas</i> were enriched in the IM group. A predictive functional analysis revealed enrichment of the ornithine degradation pathway in the IM and dysplasia groups, suggesting its role in persistent gastric mucosal inflammation associated with gastric precancerous lesions. The gastric microbiota associated with <i>H. pylori</i>-negative gastric precancerous lesions showed an increased abundance of oral microbes linked to gastric cancer and a reduction in anti-inflammatory bacteria. These alterations might contribute to chronic gastric mucosal inflammation, promoting carcinogenesis in the absence of <i>H. pylori</i> infection.https://www.mdpi.com/2076-2607/13/1/81gastric precancerous lesionsgastric microbiota16S rRNA<i>Helicobacter pylori</i> |
| spellingShingle | Han-Na Kim Min-Jeong Kim Jonathan P. Jacobs Hyo-Joon Yang Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients Microorganisms gastric precancerous lesions gastric microbiota 16S rRNA <i>Helicobacter pylori</i> |
| title | Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients |
| title_full | Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients |
| title_fullStr | Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients |
| title_full_unstemmed | Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients |
| title_short | Gastric Microbiota Associated with Gastric Precancerous Lesions in <i>Helicobacter pylori</i>-Negative Patients |
| title_sort | gastric microbiota associated with gastric precancerous lesions in i helicobacter pylori i negative patients |
| topic | gastric precancerous lesions gastric microbiota 16S rRNA <i>Helicobacter pylori</i> |
| url | https://www.mdpi.com/2076-2607/13/1/81 |
| work_keys_str_mv | AT hannakim gastricmicrobiotaassociatedwithgastricprecancerouslesionsinihelicobacterpyloriinegativepatients AT minjeongkim gastricmicrobiotaassociatedwithgastricprecancerouslesionsinihelicobacterpyloriinegativepatients AT jonathanpjacobs gastricmicrobiotaassociatedwithgastricprecancerouslesionsinihelicobacterpyloriinegativepatients AT hyojoonyang gastricmicrobiotaassociatedwithgastricprecancerouslesionsinihelicobacterpyloriinegativepatients |