Enhancement effects of chicken interleukin-2 on immune responses of H<sub>5</sub> subtype avian influenza virus vaccines
In order to boost immune response of conventional vaccines, investigation was made on immunoadjuvants-eukaryotic expression plasmid (pCI-IL-2) and recombinant chicken IL-2 (rChIL-2) expressed in E. coli. The adjuvants were coadministrated with H<sub>5</sub> subtype inactivated avian infl...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2005-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.06.0797 |
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| Summary: | In order to boost immune response of conventional vaccines, investigation was made on immunoadjuvants-eukaryotic expression plasmid (pCI-IL-2) and recombinant chicken IL-2 (rChIL-2) expressed in E. coli. The adjuvants were coadministrated with H<sub>5</sub> subtype inactivated avian influenza virus (AIV) vaccines. Non-immunized commercial chickens were injected initially at 25-day-old and boosted 2 weeks later. Proliferation responses of chicken periphery blood lymphocytes were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay and the titers of specific antibodies to the H<sub>5</sub> vaccine by hemagglutinin inhibit (HI) test. As the results revealed, the cellular and humoral immune responses were significantly enhanced in the groups co-immunized with pCI-IL-2 or rChIL-2 compared to the group inoculated with H<sub>5</sub> vaccine only. No significant difference were found between the two co-immunized groups (P<0.05). Peak values of MTT and HI in the rChIL-2 coinoculated group were attained 4 weeks after initial inoculation, which was earlier than the group coinoculated with pCI-IL-2. These results presented evidence that both pCI-IL-2 and rChIL-2 could be employed as effective immunoadjuvant to conventional vaccines against avian influenza. |
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| ISSN: | 1008-9209 2097-5155 |