A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE
This study presents a sustainable high-performance liquid chromatography photodiode array detection (HPLC-DAD) method for analyzing polar phenolic compounds in sweet orange (Citrus sinensis) residues. Fractional factorial design was used to identify significant variables affecting method efficiency,...
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Sociedade Brasileira de Química
2025-01-01
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| Series: | Química Nova |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000300317&lng=en&tlng=en |
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| author | Mirella T. Ferreira Ranzeti Gabriela Cremasco Daniel Rinaldo |
| author_facet | Mirella T. Ferreira Ranzeti Gabriela Cremasco Daniel Rinaldo |
| author_sort | Mirella T. Ferreira Ranzeti |
| collection | DOAJ |
| description | This study presents a sustainable high-performance liquid chromatography photodiode array detection (HPLC-DAD) method for analyzing polar phenolic compounds in sweet orange (Citrus sinensis) residues. Fractional factorial design was used to identify significant variables affecting method efficiency, which were subsequently optimized using Doehlert design. The optimized conditions were: initial ethanol percentage = 20%, final ethanol percentage = 75.3%, analysis temperature = 40 °C, percentage of acetic acid in water = 0.4%, flow rate = 0.5 mL min-1, time = 35 min, and C-18 column (250 × 4.6 mm). The method effectively separated and allowed the identification of phenolic acids, vitexin, and hesperidin, while adhering to green chemistry principles, making it a safer and more environmentally friendly alternative to traditional methods, complying with principles 2, 4, 5, 6, 7, 8, 9, 10, 11 and 12 of the Green Analytical Chemistry (GAC), bringing a low cost of analysis, with little energy expenditure, low toxic solvents from renewable sources, little waste generation and safety in operation. |
| format | Article |
| id | doaj-art-a3a524b862d1416ab2f4eb72a5ee6ce5 |
| institution | Kabale University |
| issn | 1678-7064 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj-art-a3a524b862d1416ab2f4eb72a5ee6ce52025-02-04T07:37:07ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642025-01-0148310.21577/0100-4042.20250089A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTEMirella T. Ferreira Ranzetihttps://orcid.org/0000-0002-0337-6148Gabriela Cremascohttps://orcid.org/0000-0002-7783-7824Daniel Rinaldohttps://orcid.org/0000-0001-5363-6481This study presents a sustainable high-performance liquid chromatography photodiode array detection (HPLC-DAD) method for analyzing polar phenolic compounds in sweet orange (Citrus sinensis) residues. Fractional factorial design was used to identify significant variables affecting method efficiency, which were subsequently optimized using Doehlert design. The optimized conditions were: initial ethanol percentage = 20%, final ethanol percentage = 75.3%, analysis temperature = 40 °C, percentage of acetic acid in water = 0.4%, flow rate = 0.5 mL min-1, time = 35 min, and C-18 column (250 × 4.6 mm). The method effectively separated and allowed the identification of phenolic acids, vitexin, and hesperidin, while adhering to green chemistry principles, making it a safer and more environmentally friendly alternative to traditional methods, complying with principles 2, 4, 5, 6, 7, 8, 9, 10, 11 and 12 of the Green Analytical Chemistry (GAC), bringing a low cost of analysis, with little energy expenditure, low toxic solvents from renewable sources, little waste generation and safety in operation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000300317&lng=en&tlng=enGreen ChemistryHPLC-DADphenolic compoundsorange peel wastebioeconomy |
| spellingShingle | Mirella T. Ferreira Ranzeti Gabriela Cremasco Daniel Rinaldo A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE Química Nova Green Chemistry HPLC-DAD phenolic compounds orange peel waste bioeconomy |
| title | A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE |
| title_full | A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE |
| title_fullStr | A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE |
| title_full_unstemmed | A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE |
| title_short | A GREEN AND EFFICIENT HPLC-DAD METHOD FOR THE ANALYSIS OF PHENOLIC COMPOUNDS IN ORANGE WASTE |
| title_sort | green and efficient hplc dad method for the analysis of phenolic compounds in orange waste |
| topic | Green Chemistry HPLC-DAD phenolic compounds orange peel waste bioeconomy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422025000300317&lng=en&tlng=en |
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