Quantifying antibody binding on protein microarrays using microarray nonlinear calibration

Quantifying antibody binding on protein microarrays using microarray nonlinear calibration

We present a microarray nonlinear calibration (MiNC) method for quantifying antibody binding to the surface of protein microarrays that significantly increases the linear dynamic range and reduces assay variation compared with traditional approaches. A serological analysis of guinea pig Mycobacteriu...

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Bibliographic Details
Main Authors: Xiaobo Yu, Garrick Wallstrom, Dewey Mitchell Magee, Ji Qiu, D. Eliseo A. Mendoza, Jie Wang, Xiaofang Bian, Morgan Graves, Joshua LaBaer
Format: Article
Language:English
Published: Taylor & Francis Group 2013-05-01
Series:BioTechniques
Subjects:
microarray nonlinear calibration
nucleic acid programmable protein arrays
nn vitro transcription/translation
tuberculosis
Online Access:https://www.future-science.com/doi/10.2144/000114028
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https://www.future-science.com/doi/10.2144/000114028

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