Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats
Our previous studies suggest that reactive oxygen species (ROS) scavengers have analgesic effect on neuropathic pain through spinal mechanisms in the rat. The studies suggest that superoxide in spinal cord is one of important mediators of persistent pain. To test the hypothesis that increase of supe...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/293423 |
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| author | Hee Young Kim Inhyung Lee Sang Woo Chun Hee Kee Kim |
| author_facet | Hee Young Kim Inhyung Lee Sang Woo Chun Hee Kee Kim |
| author_sort | Hee Young Kim |
| collection | DOAJ |
| description | Our previous studies suggest that reactive oxygen species (ROS) scavengers have analgesic effect on neuropathic pain through spinal mechanisms in the rat. The studies suggest that superoxide in spinal cord is one of important mediators of persistent pain. To test the hypothesis that increase of superoxide-derived intermediates leads to central sensitization and pain, the effects of an intrathecal injection of chemical ROS donors releasing either OH∙, OCl-, or H2O2 were examined on pain behaviors. Following treatment with t-BOOH (OH∙ donor), dorsal horn neuron responses to mechanical stimuli in normal rats and the changes of neuronal excitability were explored on substantia gelatinosa (SG) neurons using whole-cell patch clamping recordings. Intrathecal administration of t-BOOH or NaOCl (OCl- donor), but not H2O2, significantly decreased mechanical thresholds of hind paws. The responses of wide dynamic range neurons to mechanical stimuli increased after a local application of t-BOOH. The t-BOOH increased the frequency and the amplitude of excitatory postsynaptic potentials, depolarized membrane potential in SG neurons, and increased the frequency of action potentials evoked by depolarizing current pulses. These results suggest that elevated ROS, especially OH∙, in the spinal cord sensitized dorsal horn neurons and produced hyperalgesia in normal rats. |
| format | Article |
| id | doaj-art-a36b4ab0dad94beb94adf07c70cba182 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | Neural Plasticity |
| spelling | doaj-art-a36b4ab0dad94beb94adf07c70cba1822025-02-03T01:20:41ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/293423293423Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in RatsHee Young Kim0Inhyung Lee1Sang Woo Chun2Hee Kee Kim3Department of Neuroscience and Cell Biology, 301 University Boulevard, University of Texas Medical Branch, TX 77555-1069, USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of KoreaDepartment of Oral Physiology, College of Dentistry, Institute of Wonkwang Biomaterial and Implant, Wonkwang University, 344-2 Shinyong Dong, Iksan 570-749, Republic of KoreaDepartment of Pain Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USAOur previous studies suggest that reactive oxygen species (ROS) scavengers have analgesic effect on neuropathic pain through spinal mechanisms in the rat. The studies suggest that superoxide in spinal cord is one of important mediators of persistent pain. To test the hypothesis that increase of superoxide-derived intermediates leads to central sensitization and pain, the effects of an intrathecal injection of chemical ROS donors releasing either OH∙, OCl-, or H2O2 were examined on pain behaviors. Following treatment with t-BOOH (OH∙ donor), dorsal horn neuron responses to mechanical stimuli in normal rats and the changes of neuronal excitability were explored on substantia gelatinosa (SG) neurons using whole-cell patch clamping recordings. Intrathecal administration of t-BOOH or NaOCl (OCl- donor), but not H2O2, significantly decreased mechanical thresholds of hind paws. The responses of wide dynamic range neurons to mechanical stimuli increased after a local application of t-BOOH. The t-BOOH increased the frequency and the amplitude of excitatory postsynaptic potentials, depolarized membrane potential in SG neurons, and increased the frequency of action potentials evoked by depolarizing current pulses. These results suggest that elevated ROS, especially OH∙, in the spinal cord sensitized dorsal horn neurons and produced hyperalgesia in normal rats.http://dx.doi.org/10.1155/2015/293423 |
| spellingShingle | Hee Young Kim Inhyung Lee Sang Woo Chun Hee Kee Kim Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats Neural Plasticity |
| title | Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats |
| title_full | Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats |
| title_fullStr | Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats |
| title_full_unstemmed | Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats |
| title_short | Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats |
| title_sort | reactive oxygen species donors increase the responsiveness of dorsal horn neurons and induce mechanical hyperalgesia in rats |
| url | http://dx.doi.org/10.1155/2015/293423 |
| work_keys_str_mv | AT heeyoungkim reactiveoxygenspeciesdonorsincreasetheresponsivenessofdorsalhornneuronsandinducemechanicalhyperalgesiainrats AT inhyunglee reactiveoxygenspeciesdonorsincreasetheresponsivenessofdorsalhornneuronsandinducemechanicalhyperalgesiainrats AT sangwoochun reactiveoxygenspeciesdonorsincreasetheresponsivenessofdorsalhornneuronsandinducemechanicalhyperalgesiainrats AT heekeekim reactiveoxygenspeciesdonorsincreasetheresponsivenessofdorsalhornneuronsandinducemechanicalhyperalgesiainrats |