A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration
Multipotent stem cells have the capacity to generate terminally differentiated cell types of each lineage; thus, they have great therapeutic potential for a wide variety of diseases. The most widely available stem cells are derived from human tissues, and their use for therapeutic application is lim...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3891404 |
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| _version_ | 1832555939947872256 |
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| author | Minkoo Seo Jin-Chul Kim Hyung-Ki Kim Eun wook Choi Suyeong Jeong Ki Chang Nam Mihue Jang |
| author_facet | Minkoo Seo Jin-Chul Kim Hyung-Ki Kim Eun wook Choi Suyeong Jeong Ki Chang Nam Mihue Jang |
| author_sort | Minkoo Seo |
| collection | DOAJ |
| description | Multipotent stem cells have the capacity to generate terminally differentiated cell types of each lineage; thus, they have great therapeutic potential for a wide variety of diseases. The most widely available stem cells are derived from human tissues, and their use for therapeutic application is limited by their high cost and low productivity. Herein, we report that conditioned media of mesenchymal stem cells (MSCs) isolated from deer antlers enhanced tissue regeneration through paracrine action via a combination of secreted growth factors and cytokines. Notably, DaMSC-conditioned media (DaMSC-CM) enhanced hair regeneration by activating the Wnt signaling pathway. In addition, DaMSC-CM had regenerative potential in damaged skin tissue through induction of skin regeneration-related genes. Remarkably, we identified round vesicles derived from DaMSC-CM, with an average diameter of ~120 nm that were associated with hair follicle formation, suggesting that secretory vesicles may act as paracrine mediators for modulation of local cellular responses. In addition, these secretory vesicles could regulate the expression of Wnt-3a, Wnt-10b, and lymphoid enhancer-binding factor-1 (LEF-1), which are related to tissue renewal. Thus, our findings demonstrate that the use of DaMSC-CM as a unique natural model for rapid and complete tissue regeneration has possible application for therapeutic development. |
| format | Article |
| id | doaj-art-a35557df148a48c19e4a0b88150c863e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a35557df148a48c19e4a0b88150c863e2025-02-03T05:46:48ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/38914043891404A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue RegenerationMinkoo Seo0Jin-Chul Kim1Hyung-Ki Kim2Eun wook Choi3Suyeong Jeong4Ki Chang Nam5Mihue Jang6Prostemics Research Institute, Seongdong-gu, Seoul, Republic of KoreaNatural Constituents of Research Center, Natural Products Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of KoreaProstemics Research Institute, Seongdong-gu, Seoul, Republic of KoreaProstemics Research Institute, Seongdong-gu, Seoul, Republic of KoreaProstemics Research Institute, Seongdong-gu, Seoul, Republic of KoreaDepartment of Medical Engineering, Dongguk University College of Medicine, Seoul, Republic of KoreaCenter for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of KoreaMultipotent stem cells have the capacity to generate terminally differentiated cell types of each lineage; thus, they have great therapeutic potential for a wide variety of diseases. The most widely available stem cells are derived from human tissues, and their use for therapeutic application is limited by their high cost and low productivity. Herein, we report that conditioned media of mesenchymal stem cells (MSCs) isolated from deer antlers enhanced tissue regeneration through paracrine action via a combination of secreted growth factors and cytokines. Notably, DaMSC-conditioned media (DaMSC-CM) enhanced hair regeneration by activating the Wnt signaling pathway. In addition, DaMSC-CM had regenerative potential in damaged skin tissue through induction of skin regeneration-related genes. Remarkably, we identified round vesicles derived from DaMSC-CM, with an average diameter of ~120 nm that were associated with hair follicle formation, suggesting that secretory vesicles may act as paracrine mediators for modulation of local cellular responses. In addition, these secretory vesicles could regulate the expression of Wnt-3a, Wnt-10b, and lymphoid enhancer-binding factor-1 (LEF-1), which are related to tissue renewal. Thus, our findings demonstrate that the use of DaMSC-CM as a unique natural model for rapid and complete tissue regeneration has possible application for therapeutic development.http://dx.doi.org/10.1155/2018/3891404 |
| spellingShingle | Minkoo Seo Jin-Chul Kim Hyung-Ki Kim Eun wook Choi Suyeong Jeong Ki Chang Nam Mihue Jang A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration Stem Cells International |
| title | A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration |
| title_full | A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration |
| title_fullStr | A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration |
| title_full_unstemmed | A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration |
| title_short | A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration |
| title_sort | novel secretory vesicle from deer antlerogenic mesenchymal stem cell conditioned media damsc cm promotes tissue regeneration |
| url | http://dx.doi.org/10.1155/2018/3891404 |
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